Comparative hyperaccumulator of Azolla pinnata and Lemna minor for livestock wastewater treatment: morpho-physiological and genetic approach

Abstract

The potential of two different aquatic macrophytes, A. pinnata and L. minor to treat livestock wastewater through phytoremediation were investigated. The physiological includes the removal efficiency of Manganese (Mn) from livestock wastewater by AAS, morphological observation was performed under visual and SEM. RAPD analysis were applied to observe the DNA expression. It was observed that the removal efficiency of Mn, was higher in L. minor with 92% removal rate, while in A.pinnata RE was at 77% rate. A.pinnata exhibits symptoms of cell death by shrinking when exposed to livestock effluent as early as 24 hours but L.minor showed no changes. RAPD analysis showed that there are 19% of polymorphism in L.minor, in A.pinnata there is increase in band intensities. It can be concluded that L. minor performed better as a hyperaccumulator in livestock wastewater than A. pinnata which exhibits signs of cell death.