Comparative
            <i>In Vitro</i>
            Study of Biofilm Formation and Antimicrobial Susceptibility in Gram-Negative Bacilli Isolated from Prosthetic Joint Infections

Macías-Valcayo, Alicia; Aguilera-Correa, John Jairo; Broncano, Antonio; Parrón, R.; Auñón, Álvaro; García-Cañete, Joaquin; Blanco, Antonio; Esteban, Jaime

doi:10.1128/spectrum.00851-22

Cited by 13 publications

(5 citation statements)

References 46 publications

(57 reference statements)

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“…Patients with IRIs caused by gram‐negative bacteria often have a worse prognosis than those with IRIs caused by gram‐positive bacteria, even after sufficient treatment. [ 16 ] The barrier effect of biofilms means that the direct effect of antibiotics is resisted. However, the cell membrane component of E. coli OMVs in HMLS increases the specificity of nanoparticles, as HMLS can be effectively internalized by bacteria, improving drug efficiency.…”

Section: Resultsmentioning

confidence: 99%

Bacterial Outer Membrane‐Based Biomimetic Immune Adaptors: Mild Immunomodulatory and Bacterial Targeted Delivery Strategy Against Implant‐Related Infections

Ding

Cheng

et al. 2023

Adv Funct Materials

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Implant‐related infections (IRIs) are difficult to manage and are a major cause of surgical failure. The formation of biofilms with a solid barrier on the surface of implants and concomitant suppression of the local immune response constitute major challenges for treating IRIs. This study develops a multifunctional biomimetic immune adaptor (Hybrid Membrane@Levo@SiO2; HMLS) generated by loading the antibiotic levofloxacin (Levo) into silica nanoparticles and coating the particles with a hybrid membrane formed from outer membrane vesicles (OMVs) of Escherichia coli and red blood cell membranes (RBCMs). The HMLS nanoplatform is designed to address the challenges associated with IRIs through a dual‐approach strategy. First, the unique immunogenicity of OMVs regulates the antibacterial immune responses of innate immune cells and reverses the immunosuppressive microenvironment around IRIs. Second, the homotypic targeting effect of OMVs in the hybrid membrane provides HMLS with bacterial targeting functions and efficient bactericidal effects. Additionally, the RBCMs endow the hybrid membrane with mild immunogenicity, which prevents excessive inflammatory stimulation. The synergistic strategy of HMLS exhibits superior antibiofilm effects both in vivo and in vitro, providing a new option for the clinical treatment of refractory IRIs.

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Section: Resultsmentioning

confidence: 99%

Bacterial Outer Membrane‐Based Biomimetic Immune Adaptors: Mild Immunomodulatory and Bacterial Targeted Delivery Strategy Against Implant‐Related Infections

Ding

Cheng

et al. 2023

Adv Funct Materials

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“…[27] reported that 35.2% were strong biofilm forming isolates, 16.7% were moderate biofilm forming and 27.8% were weak biofilm forming, the different methods used for assessment of the strength of biofilm formation could be a potential cause for this variation. Macias-Valcayo et al (2022) [24] also reported different results where 41.3% of their isolates were strong producers, 34.8% were moderate and 21.8% were weak biofilm-producers.…”

Section: Table (2) and Figure (4)mentioning

confidence: 96%

“…(Table 3) and (Figure 5). ---------------------------------------------------- [22] studies where (64.28%) and (62.73%) of isolates were biofilm forming, respectively, Baidya et al (2021) [23] reported biofilm-formation in 56.3% of their isolates, and Macias-Valcayo et al (2022) [24] reported that most of Gram negative strains of HAIs were biofilm producers (97%).The results of Almalki and Varghese (2020) [25] where different from our results where they reported that (63.9%) of isolates were non-biofilm forming while biofilm forming isolates were (36.1%), which could be attributed to the difference in the species of Gram negative isolates and the single potential of every pathogen to form biofilms.…”

Section: Table (2) and Figure (4)mentioning

confidence: 98%

Anti-biofilm Effect of Copper Oxide Nanoparticles, EDTA, and Kojic Acid on MDR-biofilm Forming Gram Negative Superbugs in Sohag University Hospitals, Egypt

Thabet

Esmail

Esmat

2022

Microbes and Infectious Diseases

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Background: Multidrug-resistant (MDR) Gram negative superbugs are considered among the most important causes of healthcare associated infections and these resistant isolates are usually present in association with biofilms rather than planktonic existence. Objectives: This study aimed to assess the ability of EDTA, Kojic acid, and CuONPs to inhibit the phenomenon of biofilm formation by multidrug-resistant Gram-negative superbugs. Methodology: Gram negative bacilli were isolated on suitable media and identified biochemically by vitek-2 biochemical identification system. MDR isolates were tested for biofilm formation by tissue culture plate method, biofilm-producing isolates were exposed EDTA and Kojic acid by certain concentration and to copper oxide nanoparticle (CuONPs) by multiple gradual concentrations and re-tested for biofilm-production by the same method. Results: EDTA and Kojic acid reduced the biofilm-formation ability of the tested isolates by 85.4 % and 65.5% respectively with reduced mean optical density (OD) reading to (0.0267± 0.051) and (0.063± 0.108) respectively with a high statistically significant difference (p<0.0001). A concentrationdependent biofilm-inhibition effect was observed with different concentrations of CuO NPs (125-2000 µg/ml) for all the tested isolates regardless the genus and the species. Conclusion: there was a remarkable biofilm-inhibition effect for the three tested non-antibiotic substances; the use of these substances can provide a solution for the problem of difficulty in treatment of nosocomial infections due to MDR biofilm-forming Gram-negative superbugs.

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“…This phenomenon is primarily responsible for the lack of therapeutic success with systemic antibiotics, since biofilm confers antimicrobial tolerance, reduces antibiotic diffusion and penetration, and evades the host immune response. Recently, it has been described that the extracellular matrix is one of the main factors responsible for resistance, in addition to other intrinsic factors specific to each strain that directly affect biofilm formation and cell dispersion [26][27][28][29][30][31].…”

Section: Question 5 What Are the Consequences Of Cri In Terms Of Morb...mentioning

confidence: 99%

Vascular catheter-related infections: an endemic disease in healthcare institutions. An opinion paper of the Spanish Society of Cardiovascular Infections (SEICAV)

Muñoz,

Guembe,

Pérez-Granda

et al. 2024

Rev Esp Quimioter

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Catheter-related infections (CRI) are a serious healthcare problem due to their potential to cause serious complications, including bacteraemia or infective endocarditis, and to increase patient morbidity and mortality. In addition, these in fections significantly prolong hospital stay and cost. Preventing CRI is crucial and is considered a criterion for quality and safety in healthcare. For these reasons, the Spanish Society of Cardiovascular Infections (SEICAV) has considered it pertinent to review this topic, with experts in different areas including clinical microbiologists, infectious disease specialists, surgeons and nurses. The data were presented at a session held at the Ramón Areces Foundation, which was organised in the form of specific questions grouped into three round tables. The first panel analysed the scale of the problem including epidemiological, clinical and diagnostic aspects; the second panel addressed advances in the treatment of CRI; and the third panel reviewed developments in the prevention of CRI. The recorded session is available on the Areces Foundation website and we believe it maybe of interest not only to health professionals, but also to any non-expert citizen interested in the subject.

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Comparative In Vitro Study of Biofilm Formation and Antimicrobial Susceptibility in Gram-Negative Bacilli Isolated from Prosthetic Joint Infections

Cited by 13 publications

References 46 publications

Bacterial Outer Membrane‐Based Biomimetic Immune Adaptors: Mild Immunomodulatory and Bacterial Targeted Delivery Strategy Against Implant‐Related Infections

Bacterial Outer Membrane‐Based Biomimetic Immune Adaptors: Mild Immunomodulatory and Bacterial Targeted Delivery Strategy Against Implant‐Related Infections

Anti-biofilm Effect of Copper Oxide Nanoparticles, EDTA, and Kojic Acid on MDR-biofilm Forming Gram Negative Superbugs in Sohag University Hospitals, Egypt

Vascular catheter-related infections: an endemic disease in healthcare institutions. An opinion paper of the Spanish Society of Cardiovascular Infections (SEICAV)

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