Comparative Immunochemical Studies of the Surface Antigens of Escherichia coli Strains O8:K87(B?):H19 and (O32):K87(B?):H45

Abstract

The O-antigenic lipopolysaccharides and the acidic polysaccharides isolated from Eschrichia coli strains 0 8 : K87(B 2) : H45 were compared with respect to chemical composition and serological specificity.The O-specific polysaccharide moiety of the lipopolysaccharide from E . coli 08 :K87(B ?) : Hi9 contains mannose as the sole sugar constituent, whereas the (032)-specific polysaccharide moiety of the lipopolysaccharide from E . coli (032) :K87(B 2 ) :H45 consists of glucose, galactose, glucuronic acid, N-acetylglucosamine, and N-acetyl-L-fucosamine in the molar ratios of 1 : 1 : 1 : 1 : I. From both strains, an acidic polysaccharide can be isolated which consists of glucose, galactose, glucuronic acid, N-acetylglucosamine, and N-acetyl-L-fucosamine in the molar ratios of 1 : 1 : 1 : 1 : I. Both acidic-polysaccharide preparations, as well as the (032)-specific polysaccharide moiety of the lipopolysaccharide from E . coli (032) : K87(B 2) : H45, have the same structure and, upon partial acid hydrolysis, yield the same oligosaccharides.With the aid of bacterial agglutination, passive haemagglutination, and immune precipitation in homologous and absorbed immune sera, as well as inhibition studies of these reactions with oligosaccharides, serological identity of the acidic polysaccharide and the (032)-specific polysaccharide moiety of the lipopolysaccharide from E . coli (032) : K87(B 2) : H45 was shown.It is concluded that the acidic polysaccharide, which has been shown to be the K a.ntigen of E . coli 08 :K87(B 2 ) :Hl9, is also responsible for O-specificity in strain (032) :K87(B 2) :H45.

A Wzz (Cld) protein determines the chain length of K lipopolysaccharide in Escherichia coli O8 and O9 strains

“…For G7 or Bi 316-42 we suggest that at some time in the past, the major O-antigen structures were O32 and O104, respectively, but during adaptation to a new niche, the O8 or the O9 antigen was acquired and became the major LPS polysaccharide, with the O32 or O104 antigen converted to a capsule, although with a proportion still present in LPS as K LPS . This hypothesis is supported by the fact that the structures of capsules K87 and K9 are identical to those of O32 and O104, respectively (12,20).…”

Somatic Antigen of Shigella dysenteriae Type 3. Structural Features of Specific Polysaccharide Chain

“…boydii type 6 [19] lipopolysaccharides indicated that the polysaccharide chain of certain Shigellu lipopolysaccharides from serogroups A, C and D could be the acidic ones. Till the recent time, the occurrence of acidic polysaccharide chains was ascertained only in lipopolysaccharides of some Escherichia coli strains [20]. The charged (capsular) polysaccharides are known to endow the bacteria with the resistance to host defence mechanisms [21,22].…”

Serology, chemistry, and genetics of O and K antigens of Escherichia coli.

“…In this context it is interesting to note that in E. coli strains 08 and 09 no rfc locus (determining the polymerase for lipidlinked oligosaccharides [see reference 279]) was found. MULTIPLE OCCURRENCE OF POLYSAC-CHARIDE ANTIGENS E. coli strain D227, 08:K87 was originally believed to contain a neutral cell wall LPS and, in addition, an acidic capsular polysaccharide (108,113,210,286). It was found later (Jann and Jann, manuscript in preparation) that the K87 antigen was actually an acidic LPS, so the strain contains two LPS in the outer membrane.…”