Comparative immunocytochemical study of the subcommissural organ

Rodríguez, Estéban M.; Oksche, A.; Hein, Silvia; Rodrı́guez, Sara; Yulis, Roberto

doi:10.1007/bf00228427

Cited by 138 publications

(126 citation statements)

References 21 publications

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“…This organ produces Reissner's fibers, and both the organ and the fibers have been shown to be important for the patency of the aqueduct, in that destruction of the SCO leads to obstructive hydrocephalus (Perez-Figares et al, 2001). Antibodies specific to Reissner's fibers (Rodriguez et al, 1984;Rodriguez et al, 2001; strongly and specifically labeled the SCO from the wild-type mice (Fig. 3B), but this label was generally not detected in the same anatomical region in the transgenic mice.…”

Section: Evaluation Of Transgenic Micementioning

confidence: 95%

“…They were then embedded in paraffin wax, sectioned and stained with Hematoxylin/Eosin by standard methods. For immunohistochemistry, paraffin wax-embedded sections were stained with an antibody (Rodriguez et al, 1984) to Reissner's fibers (RF) within the SCO, as described previously for a different antibody (Blackshear et al, 1996). The anti-RF antibody was a generous gift from Dr E. M. Rodriguez (Instituto de Histologia y Patologia, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile).…”

Section: Histologymentioning

confidence: 99%

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Graded phenotypic response to partial and complete deficiency of a brain-specific transcript variant of the winged helix transcription factor RFX4

et al. 2003

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Interruption of a single allele prevented formation of the subcommissural organ, a structure important for cerebrospinal fluid flow through the aqueduct of Sylvius, and resulted in congenital hydrocephalus. These data implicate the RFX4_v3 variant transcript as being crucial for early brain development, as well as for the genesis of the subcommissural organ. These findings may be relevant to human congenital hydrocephalus, a birth defect that affects ~0.6 per 1000 newborns.

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Section: Evaluation Of Transgenic Micementioning

confidence: 95%

Section: Histologymentioning

confidence: 99%

Graded phenotypic response to partial and complete deficiency of a brain-specific transcript variant of the winged helix transcription factor RFX4

et al. 2003

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“…The dog SCO displays the typical structure of an endocrine gland, i.e., a solid mass of secretory cells penetrated by numerous blood capillaries (Fig. 12) (Oksche, 1969;Rodríguez et al, 1984aRodríguez et al, , 1986. Many of the cells do not reach the ventricle but are in close contact with the capillaries; at their vascular pole, these cells present numerous small granules of high electron density and that are not reactive with anti-RF sera (Rodríguez et al, 1987b).…”

Section: Ultrastructure Ependymal Cellsmentioning

confidence: 97%

The subcommissural organ

Rodríguez

Rodrı́guez

Hein

1998

Microsc. Res. Tech.

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“…The pups were perfused intravascularly with saline followed by Bouin's fixative, and the brains were excised, cryoprotected, and frozen for sectioning at 20 µm on a cryostat. Coronal sections containing the aqueduct and SCO were immunostained with the antibody to Reissner's fiber glycoprotein (AFRU, Rodríguez et al 1984). The aqueduct was examined for signs of narrowing or nonpatency and the dorsal regions, including the SCO, were studied for immunostaining.…”

Section: Histological Examination-h-tx Rats With Hydrocephalus Have Amentioning

confidence: 99%

Single and multiple congenic strains for hydrocephalus in the H-Tx rat

et al. 2005

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The H-Tx rat has fetal-onset hydrocephalus with a complex mode of inheritance. Previously, quantitative trait locus mapping using a backcross with Fischer F344 rats demonstrated genetic loci significantly linked to hydrocephalus on Chromosomes 10, 11, and 17. Hydrocephalus was preferentially associated with heterozygous alleles on Chrs 10 and 11 and with homozygous alleles on Chr 17. This study aimed to determine the phenotypic contribution of each locus by constructing single and multiple congenic strains. Single congenic rats were constructed using Fischer F344 as the recipient strain and a marker-assisted protocol. The homozygous strains were maintained for eight generations and the brains examined for dilated ventricles indicative for hydrocephalus. No congenic rats had severe (overt) hydrocephalus. A few pups and a significant number of adults had mild disease. The incidence was significantly higher in the C10 and C17 congenic strains than in the nonhydrocephalic F344 strain. Breeding to F344 to make F.H-Tx C10 or C11 rats heterozygous for the hydrocephalus locus failed to produce progeny with severe disease. Both bicongenic and tricongenic rats of different genotype combinations were constructed by crossing congenic rats. None had severe disease but the frequency of mild hydrocephalus in adults was similar to congenic rats and significantly higher than in the F344 strain. Rats with severe hydrocephalus were recovered in low numbers when single congenic or bicongenic rats were crossed with the parental H-Tx strain. It is concluded that the genetic and epigenetic factors contributing to severe hydrocephalus in the HTx strain are more complex than originally anticipated.

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Comparative immunocytochemical study of the subcommissural organ

Cited by 138 publications

References 21 publications

Graded phenotypic response to partial and complete deficiency of a brain-specific transcript variant of the winged helix transcription factor RFX4

Graded phenotypic response to partial and complete deficiency of a brain-specific transcript variant of the winged helix transcription factor RFX4

The subcommissural organ

Single and multiple congenic strains for hydrocephalus in the H-Tx rat

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