Comparative In Vivo Analysis of the Integration Behavior and Immune Response of Collagen-Based Dental Barrier Membranes for Guided Bone Regeneration (GBR)

Radenković, Milena; Alkildani, Said; Stoewe, Ignacio; Bielenstein, James; Sundag, Bernd; Bellmann, O.; Jung, Ole; Najman, Stevo; Stojanović, Sanja; Barbeck, Mike

doi:10.3390/membranes11090712

Cited by 39 publications

(59 citation statements)

References 48 publications

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“…A broad variety of mostly preclinical studies have been conducted to analyze the cellular migration pattern of barrier membranes both in vitro and in vivo [6,20,36,37]. However, only qualitative data regarding this topic are normally reported, as no quantitative methodology to measure the cell migration behavior has been reported until now [6,20,36,37].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, materials research is constantly searching for methods (such as various cross-linking techniques) to extend the service life of bioresorbable GBR membranes while maintaining a good tissue compatibility [5]. A broad variety of mostly preclinical studies have been conducted to analyze the cellular migration pattern of barrier membranes both in vitro and in vivo [6,20,36,37]. However, only qualitative data regarding this topic are normally reported, as no quantitative methodology to measure the cell migration behavior has been reported until now [6,20,36,37].…”

Section: Discussionmentioning

confidence: 99%

“…Then, the respective area of the membrane was divided into two approximately identical examination areas, each representing one half of the membrane (Figure 1). Initially, the thickness of the two halves of membranes were calculated by measuring the respective thickness as previously described [6,18]. The next step of the histomorphometrical analysis included the manual measurement of the migration depth of the cells into the membrane, starting on both outer surfaces, via the software ImageJ (Figure 1).…”

Section: Histopathological and Histomorphometrical Analysismentioning

confidence: 99%

“…Beside this cellular occlusion property, the barrier membrane should provide space maintenance, preventing the soft tissue from falling into the defect area which can lead to increased inflammation and ultimately, implantation failure [5]. Alongside these traditional properties, special properties of the barrier membranes are necessary and currently being intensively researched [6,7]: (i) biocompatibility: is responsible for cell attachment and tissue integration, reduces or prevents inflammatory degradation processes, (ii) handling: safe clinical applicability and easy intraoperative handling, (iii) wound healing: stabilization of blood clot, and (iv) tissue integration: transmembrane angiogenesis (enables early ingrowth of blood vessels).…”

Section: Introductionmentioning

confidence: 99%

“…As a current standard, cellular infiltration (as a function of barrier functionality) of barrier membranes is only qualitatively analyzed through histopathological methods [6,33,34]. This analysis is done through the identification of the different cells of the immune system, their location at the surface/within the biomaterial, presence of multinucleated giant cells (MNGCs), and the occurrence of fibrosis/neovascularization/necrosis [18].…”

Section: Introductionmentioning

confidence: 99%

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Novel Histomorphometrical Approach to Evaluate the Integration Pattern and Functionality of Barrier Membranes

Ottenbacher

Alkildani²,

Koržinskas³

et al. 2021

Dentistry Journal

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GBR (guided bone regeneration) is a standard procedure for building up bony defects in the jaw. In this procedure, resorbable membranes made of bovine and porcine collagen are increasingly being used, which, in addition to many possible advantages, could have the potential disadvantage of a shorter barrier functionality, especially when augmenting large-volume defects. Thus, it is of importance to evaluate the integration behavior and especially the standing time of barrier membranes using specialized methods to predict its respective biocompatibility. This study is intended to establish a new histomorphometrical analysis method to quantify the integration rate of collagen-based barrier membranes. Three commercially available barrier membranes, i.e., non-crosslinked membranes (BioGide® and Jason® membrane), a ribose-crosslinked membrane (Ossix® Plus), and a newly developed collagen–hyaluronic acid-based (Coll-HA) barrier membrane were implanted in the subcutaneous tissue of 48 6–8-week-old Wistar rats. The explants, after three timepoints (10, 30, and 60 days), were processed and prepared into histological sections for histopathological (host tissue response) and histomorphometrical (cellular invasion) analyses. 10 days after implantation, fragmentation was not evident in any of the study groups. The sections of the Coll-HA, Jason® and BioGide® membranes showed a similar mild inflammatory reaction within the surrounding tissue and an initial superficial cell immigration. Only in the Ossix® Plus group very little inflammation and no cell invasion was detected. While the results of the three commercially available membranes remained intact in the further course of the study, only fragments of the Coll-HA membrane were found 30 and 60 days after implantation. Histomorphometrically, it can be described that although initially (at 10 days post-implantation) similar results were found in all study groups, after 30 days post-implantation the cellular penetration depth of the hyaluronic acid-collagen membrane was significantly increased with time (**** p < 0.0001). Similarly, the percentage of cellular invasion per membrane thickness was also significantly higher in the Coll-HA group at all timepoints, compared to the other membranes (**** p < 0.0001). Altogether, these results show that the histomorphometrical analysis of the cellular migration can act as an indicator of integration and duration of barrier functionality. Via this approach, it was possible to semi-quantify the different levels of cellular penetration of GBR membranes that were only qualitatively analyzed through histopathological approaches before. Additionally, the results of the histopathological and histomorphometrical analyses revealed that hyaluronic acid addition to collagen does not lead to a prolonged standing time, but an increased integration of a collagen-based biomaterial. Therefore, it can only partially be used in the dental field for indications that require fast resorbed membranes and a fast cell or tissue influx such as periodontal regeneration processes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Histopathological and Histomorphometrical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel Histomorphometrical Approach to Evaluate the Integration Pattern and Functionality of Barrier Membranes

Ottenbacher

Alkildani²,

Koržinskas³

et al. 2021

Dentistry Journal

Self Cite

View full text Add to dashboard Cite

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Engineering Dual Active Sites and Defect Structure in Nanozymes to Reprogram Jawbone Microenvironment for Osteoradionecrosis Therapy

Cheng,

Wang,

Lin

et al. 2024

Advanced Science

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Four to eight percent of patients with head and neck cancer will develop osteoradionecrosis of the jaw (ORNJ) after radiotherapy. Various radiation‐induced tissue injuries are associated with reactive oxygen and nitrogen species (RONS) overproduction. Herein, Fe doping is used in VOx (Fe‐VOx) nanozymes with multienzyme activities for ORNJ treatment via RONS scavenging. Fe doping can induce structure reconstruction of nanozymes with abundant defect production, including Fe substitution and oxygen vacancies (OVs), which markedly increased multiple enzyme‐mimicking activity. Catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) enzyme‐like performance of Fe‐VOx can effectively reprogram jawbone microenvironment to restore mitochondrial dysfunction and enhance mitophagy. Moreover, the surface plasmon resonance (SPR) effect of Fe‐VOx made it a good photothermal nanoagents for inhibiting jaw infection. Thus, this work demonstrated that Fe‐VOx nanozymes can efficiently scavenge RONS, activate mitophagy, and inhibit bacteria, which is potential for ORNJ treatment.

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Optimizing the combined soft tissue repair and osteogenesis using double surfaces of crosslinked collagen scaffolds

Zhang

Zhu

et al. 2023

J Biomed Mater Res

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Excessive tissue damage or loss has been solved by guided tissue regeneration and guided bone regeneration theories. However, the unfavorable degradation property of the resorbable collagen scaffold brings a big challenge to support soft tissue stabilization and time-consuming osteogenesis. The combined effect for soft tissue and bone of the collagen scaffold with better degradation pattern has not been clearly proven. This study determined whether the double surfaces of crosslinked collagen scaffolds could optimize the combined soft tissue repair and osteogenesis. In this study, we applied the chemically crosslinking treatment to the commercially available collagen scaffolds. Surface characterization, mechanical property and cell proliferation in vitro were evaluated. Combined bilateral skin and bone defects were established with the smooth surface of scaffold facing the skin defect and the rough surface facing the bone defect on the calvaria of rat. Micro-CT and histological evaluation were applied to determine the scaffold degradation pattern, soft tissue repair and osteogenesis. The crosslinked collagen scaffolds showed comparably favorable surface porosity, structure intactness, superhydrophilicity and mechanical properties.Compared to the native scaffolds, the crosslinked scaffolds could optimize the combined soft tissue repair and osteogenesis by preferably prolonged degradation time.Early pro-angiogenesis facilitated soft tissue repair and osteogenesis by upregulated soft tissue matrix degradation and balanced pro-osteogenesis with limited osteoclast-mediated bone resorption. Taken together, this study offers a promising repair strategy for the combined soft tissue and bone defects. Further, the possible mechanism of controllable scaffold degradation should be conducted.

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Comparative In Vivo Analysis of the Integration Behavior and Immune Response of Collagen-Based Dental Barrier Membranes for Guided Bone Regeneration (GBR)

Cited by 39 publications

References 48 publications

Novel Histomorphometrical Approach to Evaluate the Integration Pattern and Functionality of Barrier Membranes

Novel Histomorphometrical Approach to Evaluate the Integration Pattern and Functionality of Barrier Membranes

Engineering Dual Active Sites and Defect Structure in Nanozymes to Reprogram Jawbone Microenvironment for Osteoradionecrosis Therapy

Optimizing the combined soft tissue repair and osteogenesis using double surfaces of crosslinked collagen scaffolds

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