Comparative inhibition of .BETA.-lactamases by cephamycin antibiotics.

Toda, M; Ikeuchi, Tohru; Tajima, Masakazu; Inoue, Matsuhisa; Mitsuhashi, Susumu

doi:10.7164/antibiotics.34.114

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…This is also the case for cefoxitin and other cephamycins (27,30). These properties are well known from certain penicillin derivatives, such as methicillin, cloxacillin, or dicloxacillin (15).…”

mentioning

confidence: 86%

Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism

Then¹,

Angehrn²

1982

Antimicrob Agents Chemother

147

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Resistance to cefotaxime (CTA) and ceftriaxone (CTR) in Enterobacter cloacae and Pseudomonas aeruginosa was investigated in several strains which are susceptible or resistant to these agents. All strains produced a chromosomally mediated cephalosporinase of the Richmond type I. P-Lactamases in susceptible strains were inducible, whereas resistant strains produced the enzymes constitutively. CTA and CTR were very poor substrates but potent inhibitors of all enzymes. Binding to, rather than hydrolysis by, ,B-lactamases was assumed to be a major reason for resistance, and combination experiments supported this assumption. Dicloxacillin, which did not inhibit the growth and which was a poor inducer but a strong inhibitor of these P-lactamases, exerted strong synergistic activity when combined with CTA or CTR in strains which produced large amounts of Plactamase constitutively. Cefoxitin, on the other hand, poorly active alone, but a good inducer, strongly antagonized CTA or CTR in susceptible strains producing inducible enzymes. In marked contrast to CTA and CTR were the findings with cefsulodin. Cefsulodin was active against CTA-and CTR-resistant Pseudomonas, and its activity was hardly influenced by dicloxacillin or cefoxitin. Since cefsulodin was found to have a very low affinity for all cephalosporinases, these findings corroborate the assumption that binding of nonhydrolyzable cephalosporins, rather than hydrolysis by cephalosporinases, may play an important role in resistance to these agents and other newer cephalosporins in Enterobacteriaceae, as well as in other gram-negative bacteria.

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mentioning

confidence: 86%

Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism

Then¹,

Angehrn²

1982

Antimicrob Agents Chemother

147

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“…Cephamycins have been reported to be potent inhibitors of 3-lactamases (17). Inhibition of cephaloridine hydrolysis by cephamycins is shown in Table 4.…”

Section: Methodsmentioning

confidence: 99%

“…Because of this, these antibiotics were provided with excellent antibacterial activity against bacterial pathogens including Bacteroides fragilis. Furthermore, cephamycins have been reported to be potent competitive inhibitors of various types of 3-lactamase (17).…”

mentioning

confidence: 99%

Cephamycin inactivation due to enzymatic hydrolysis by beta-lactamase from Bacteroides fragilis

Yotsuji¹,

Minami²,

Kakizawa³

et al. 1985

Antimicrob Agents Chemother

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The susceptibility of 53 clinical isolates of Bacteroides fragilis to cephamycins was examined. Judging from the MICs for 50% of the strains tested, moxalactam was the most active, however, judging from the MICs for 90% of the strains tested, cefbuperazone was more effective than moxalactam. A correlation was observed between in vitro activity of benzylpenicillin and cephaloridine and beta-lactamase production. Inactivation due to enzymatic hydrolysis of cephamycins over a short time was not observed; however, inactivation was detected by a double disk diffusion test, and moxalactam was most easily inactivated. We conclude that inactivation due to enzymatic hydrolysis of cephamycins over a long time may play an important role in resistance to some cephamycins in strains of B. fragilis.

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“…Cefotetan, a new cephamycin antibiotic, is reported to possess not only antibacterial activity against /Mactamase-producing or ganisms, but also inhibitory activity as high as cefoxitin and cefmetazole against /9-lactamascs produced by E. coli, E. cloacae, Clos tridium freundii, Serratia marcescens, P. aeruginosa, Proteus rettgeri, Proteus morganii, Proteus vulgaris, and Bacillus jragilis [3], The antibacterial activity of cefotetan was examined against about 1,500 clini cal isolates of gram-positive and gram-ne gative bacteria at an inoculum size of 106 colony-forming units per milliliter and com pared to that of cefoxitin, cefmetazole and cefazolin [2]. Against 5. aureus and Strepto coccus pyogenes, cefotetan was slightly less active than cefoxitin, cefmetazole, and cefa zolin.…”

Section: Discussionmentioning

confidence: 99%

In vitro Comparison of Cefotetan with Five Other Cephalosporins against Nosocomial Pathogens

Daschner¹,

Goerke²,

Bassler³

et al. 1982

Chemotherapy

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The in vitro activity of cefotetan against Staphylococcus aureus and gram-negative bacterial strains including nonfermenting species has been compared with that of cefoxitin, cefotaxim, moxalactam, cefoperazone and ceftazidime by use of an agar dilution method. Cefotetan was found to be inactive against Pseudomonas aeruginosa and Acineto-bacter species and only moderately active against Staphylococcus aureus, Pseudomonas maltophilia, Pseudomonas cepacia, and Enterobacter cloacae. It was more active than cefoxitin and cefoperazone against Escherichia coli, Klebsiella pneumoniae, and indole-positive Proteus strains.

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Comparative inhibition of .BETA.-lactamases by cephamycin antibiotics.

Cited by 12 publications

References 5 publications

Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism

Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism

Cephamycin inactivation due to enzymatic hydrolysis by beta-lactamase from Bacteroides fragilis

In vitro Comparison of Cefotetan with Five Other Cephalosporins against Nosocomial Pathogens

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