Comparative Inhibitory Effects of 3‐quinuclidinyl Benzilate (Qnb) and Atropine on Amylase Release From Rat Pancreas

Morisset, J.; Ng, Karlova; Poirier, Guy G.

doi:10.1111/j.1476-5381.1977.tb09745.x

Cited by 12 publications

(6 citation statements)

References 14 publications

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“…QNB is a more potent muscarinic receptor in hibitor than atropine sulphate, Pathilon and (+) benzetimide. Dose inhibition curves for QNB and atropine sulphate have been reported pre viously (15). Figure 4A shows the correlation existing be tween (3H)-QNB displacement by agonists and their effect on amylase secretion.…”

Section: In Vitro Amylase Secretion In Response To Agonists and Antagmentioning

confidence: 99%

“…The role of muscarinic receptors con trolling pancreatic secretion has been estab lished on the basis that atropine can prevent cholinergic stimulation of acinar cell depolariza tion (12), cellular cyclic GMP accumulation (6), calcium efflux (7, 13) and enzyme secretion (14,15).…”

Section: Abstract (3h)-quinuclidiny! Benzilate [(3h)-qnb]mentioning

confidence: 99%

“…(15) in a tissue culture medium under an atmosphere of 0 ; CO, (95%-5%) as described by Beaudoin et at (3).…”

Section: In Vitro Incubation and Amylase Secretion Studiesmentioning

confidence: 99%

“…The concentrations of QNB and atropine sulphate to be used for non-specific binding were determined from the concentrations of antagonists necessary to fully inhibit the urecholine-stimulated amylase release (15).…”

Section: Binding Assaysmentioning

confidence: 99%

“…In order to characterize the pancreatic muscarinic receptors, we have previously studied the inhibitory effects of 3-quinuclidinyl benzilate (QNB) and atropine sulphate on urecholine-stimulated amylase release (15) and determined that this drug was ten times more potent than atropine sulphate. Tritiated QNB has also been used successfully to characterize similar receptors in brain and ileum (23), avian heart (17), human erythrocyte membranes (1) and axon plasma membranes (11).…”

mentioning

confidence: 99%

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Muscarinic Receptors of the Pancreas: a Correlation between Displacement of (<sup>3</sup>H)-Quinuclidinyl Benzilate Binding and Amylase Secretion

Morisset

Poirier

1979

Pharmacology

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(³H)-quinuclidinyl benzilate [(³H)-QNB], a potent muscarinic antagonist, was used as a tool to reveal the presence of muscarinic receptors in rat pancreas. Binding assays were performed on a 49,000 g pellet. Known muscarinic antagonists and agonists were used for competition assays on (³H)-QNB binding and studies on amylase secretion in vitro. A correlation was established between the binding assays and the studies on amylase release which suggests that the binding sites and those involved in the cholinergic control of pancreatic enzyme secretion are similar.

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Section: In Vitro Amylase Secretion In Response To Agonists and Antagmentioning

confidence: 99%

Section: Abstract (3h)-quinuclidiny! Benzilate [(3h)-qnb]mentioning

confidence: 99%

“…(15) in a tissue culture medium under an atmosphere of 0 ; CO, (95%-5%) as described by Beaudoin et at (3).…”

Section: In Vitro Incubation and Amylase Secretion Studiesmentioning

confidence: 99%

Section: Binding Assaysmentioning

confidence: 99%

mentioning

confidence: 99%

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Muscarinic Receptors of the Pancreas: a Correlation between Displacement of (<sup>3</sup>H)-Quinuclidinyl Benzilate Binding and Amylase Secretion

Morisset

Poirier

1979

Pharmacology

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3H-methyl scopolamine binding to dispersed pancreatic acini

et al. 1981

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Maximal amylase release occurred with 10(-5) M carbachol and slightly greater than half maximal response occurred with 3 x 10(-7) M carbachol in dispersed pancreatic acini. The preparation released more than 45% of its initial amylase content after 60 min of maximal carbachol stimulation. Electron microscopy revealed depletion of zymogen granules and the presence of secretory material in the ductules after carbachol stimulation. At 37 degrees C, maximal binding of methyl scopolamine occurred in about 45 min with 3 x 10(-10) M 3H-methyl scopolamine. The dissociation constant for 3H-methylscopolamine was 6.8 x 10(-10) M and saturation occurred at 109 pm/g protein. The I.C. 50 for 3H-methylscopolamine inhibition of carbachol-induced amylase secretion was 7 x 10(-10) M.

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Duct, exocrine, and endocrine components of cultured fetal mouse pancreas

1982

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Twenty to twenty-two days postcoitum mouse fetal pancreas organ bits were cultured on the dermal surface of irradiated pigskin as a substrate. The medium used for long term culture consisted of Eagle's Minimum Essential Medium with the addition of 10% bovine serum, 0.02 U/ml insulin, 0.025 microgram/ml glucagon, 3.63 microgram/ml hydrocortisone, 100 microgram/ml soybean trypsin inhibitor or 10(-8) M atropine. When the medium lacked trypsin inhibitor or atropine but contained the three hormones, the pigskin support began to be destroyed after 2 to 4 wk in culture. Thereafter, the cultured cells could not grow and survive on the digested pigskin. When 10(-6) M atropine was added to the medium, amylase secretion from cultured cells and destruction of pigskin were inhibited completely but pancreas cells could not grow or survive. In contrast, 100 microgram/ml soybean trypsin inhibitor or 10(-8) M atropine permitted cell growth, permitted amylase secretion from the cultured acinar cells, and prevented the destruction of pigskin. Under these conditions pancreas cells migrated or grew or both from the organ bits onto the surface of the pigskin dermis and organoid aggregations formed. Hydrocortisone was needed to permit growth for more than 2 wk. Glucagon and insulin had additive effects. Light and electron microscopic observations indicated the culture of at least five kinds of cells, i.e., duct, acinar, centroacinar, endocrine, and mesenchymal. The majority of cultured cells were duct cells and acinar cells. There were few mesenchymal cells. Mouse pancreas cells were cultured for at least 12 wk by this method.

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Comparative Inhibitory Effects of 3‐quinuclidinyl Benzilate (Qnb) and Atropine on Amylase Release From Rat Pancreas

Cited by 12 publications

References 14 publications

Muscarinic Receptors of the Pancreas: a Correlation between Displacement of (<sup>3</sup>H)-Quinuclidinyl Benzilate Binding and Amylase Secretion

Muscarinic Receptors of the Pancreas: a Correlation between Displacement of (<sup>3</sup>H)-Quinuclidinyl Benzilate Binding and Amylase Secretion

3H-methyl scopolamine binding to dispersed pancreatic acini

Duct, exocrine, and endocrine components of cultured fetal mouse pancreas

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