Comparative Investigation Between Raw and Stir-Frying Processed Cuscutae Semen Based on HPLC Fingerprints Coupled With Chemometric Methods

Xu, Changli; Shi, Yun; Hua, Xu; Guangrui, Zhao; Shurui, Niu; Yao, Jingchun; Gao, Xun; Li, Weidong; Kunming, Qin

doi:10.1093/jaoacint/qsad002

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…23 In addition, Changli et al demonstrated that the processing temperature was a factor that led to changes in the content of six phytochemical compounds (neoclorogenic acid, cryptophyllogenic acid, caffeic acid, quercetin, isorhamnetin, and kaempferol) between raw and processed Semen Cuscutae. 24…”

Section: Changes In Chemical Constituentsmentioning

confidence: 99%

Changes in Chemical Constituents and Kidney-Yang Tonifying of Traditional Herbs After Processing: A Brief Review

Truong,

Nguyen Thi,

Tran

et al. 2024

Natural Product Communications

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Background Kidney-Yang deficiency is one of the common medical syndromes in traditional medicine, characterized by symptoms such as weakness, coldness in the lower back and knees, cold, fatigue, etc. In clinical practice, Yang tonifying medicines are often used to treat patients with Kidney-Yang deficiency. Moreover, the medicinal processing method according to the principles of traditional medicine can enhance the effects on the Kidney of these herbs. This brief overview aims to gather and assess the post-processing modifications to the chemical composition and the kidney-Yang tonifying effects of specific traditional herbal remedies. Methodology Firstly, all kidney-Yang tonifying herbs were collected. Next, the selection was made based on the herbs with processing procedures specified in Circular 30/2017/TT-BYT issued by the Vietnamese Ministry of Health. All studies on the chemical composition and/or effects of processed drugs were searched in the PubMed and Google Scholar databases using the search formula: processed + scientific name of herbal medicine. Finally, among the studies on the changes in effects after processing, only studies on the tonifying Yang effect were included in this review. Results The kidney Yang-nourishing effects of the five traditional herbal medicines prescribed in Circular 30/2017/TT-BYT by the Ministry of Health of Vietnam (Radix Morindae officinalis, Cortex Eucommiae, Herba Cistanches, Semen Cuscutae, and Radix Dipsaci) have been demonstrated through several studies and summarized. From the PubMed and Google Scholar databases, 25 studies were collected for the review. Additionally, the changes in composition and content of certain active compounds in these herbal medicines have also been searched and reported. Conclusion This report provides a brief overview of the differences between chemical constituents and kidney-Yang tonifying of some traditional herbs after processing to partially contribute scientific evidence for standardizing the preparation processes and serve as an important reference for clinical practitioners of traditional medicine.

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Section: Changes In Chemical Constituentsmentioning

confidence: 99%

Changes in Chemical Constituents and Kidney-Yang Tonifying of Traditional Herbs After Processing: A Brief Review

Truong,

Nguyen Thi,

Tran

et al. 2024

Natural Product Communications

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Wine- and stir-frying processing of Cuscutae Semen enhance its ability to alleviate oxidative stress and apoptosis via the Keap 1-Nrf2/HO-1 and PI3K/AKT pathways in H2O2-challenged KGN human granulosa cell line

Liang,

Shi,

Guo

et al. 2024

BMC Complement Med Ther

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Background Cuscutae Semen (CS) has been prescribed in traditional Chinese medicine (TCM) for millennia as an aging inhibitor, an anti-inflammatory agent, a pain reliever, and an aphrodisiac. Its three main forms include crude Cuscutae Semen (CCS), wine-processed CS (WCS), and stir-frying-processed CS (SFCS). Premature ovarian insufficiency (POI) is a globally occurring medical condition. The present work sought a highly efficacious multi-target therapeutic approach against POI with minimal side effects. Finally, it analyzed the relative differences among CCS, WCS and SFCS in terms of their therapeutic efficacy and modes of action against H2O2-challenged KGN human granulosa cell line. Methods In this study, ultrahigh-performance liquid chromatography (UPLC)-Q-ExactiveTM Orbitrap-mass spectrometry (MS), oxidative stress indices, reactive oxygen species (ROS), Mitochondrial membrane potential (MMP), real-time PCR, Western blotting, and molecular docking were used to investigate the protective effect of CCS, WCS and SFCS on KGN cells oxidative stress and apoptosis mechanisms. Results The results confirmed that pretreatment with CCS, WCS and SFCS reduced H2O2-induced oxidative damage, accompanied by declining ROS levels and malondialdehyde (MDA) accumulation in the KGN cells. CCS, WCS and SFCS upregulated the expression of antioxidative levels (GSH, GSH/GSSG ratio, SOD, T-AOC),mitochondrial membrane potential (MMP) and the relative mRNA(Nrf2, Keap1, NQO-1, HO-1, SOD-1, CAT). They inhibited apoptosis by upregulating Bcl-2, downregulating Bax, cleaved caspase-9, and cleaved caspase-3, and lowering the Bax/Bcl-2 ratio. They also exerted antioxidant efficacy by partially activating the PI3K/Akt and Keap1-Nrf2/HO-1 signaling pathways. Conclusions The results of the present work demonstrated the inhibitory efficacy of CCS, WCS and SFCS against H2O2-induced oxidative stress and apoptosis in KGN cells and showed that the associated mechanisms included Keap1-Nrf2/HO-1 activation, P-PI3K upregulation, and P-Akt-mediated PI3K-Akt pathway induction.

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Comparative Investigation Between Raw and Stir-Frying Processed Cuscutae Semen Based on HPLC Fingerprints Coupled With Chemometric Methods

Cited by 2 publications

References 24 publications

Changes in Chemical Constituents and Kidney-Yang Tonifying of Traditional Herbs After Processing: A Brief Review

Changes in Chemical Constituents and Kidney-Yang Tonifying of Traditional Herbs After Processing: A Brief Review

Wine- and stir-frying processing of Cuscutae Semen enhance its ability to alleviate oxidative stress and apoptosis via the Keap 1-Nrf2/HO-1 and PI3K/AKT pathways in H2O2-challenged KGN human granulosa cell line

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