Comparative investigation of methionine and novel formulation Metovitan protective effects in Wistar rats with testicular and epididymal toxicity induced by anti-tuberculosis drugs co-administration

HAART and anti‐Koch’s impair sexual competence, sperm quality and offspring quality when used singly and in combination in male Wistar rats

“…These findings were associated with a reduced survival in offspring whose 'fathers' were treated with HAART, anti-Koch's and HAART + Anti-Koch's. These observations are in tandem with previous studies that documented reduced fertility capacity, lower litter size and weight and reduced survival of offspring of HAART and/or anti-Koch's-treated parents (Onwuamah et al, 2014;Shayakhmetova et al, 2015Shayakhmetova et al, , 2017). The reduced fertility success and fertility index are, at least partly, secondary to HAART-and Anti-Koch's-caused impaired sexual performance and poor sperm quality, while the observed lower litter size and weight might be due to an epigenetic modification with alterations in offspring quality.…”

“…Although there are no published data available to compare our findings on neither the effects of HAART and anti-Koch's on sexual competence nor the impacts of co-administration of these drugs on reproductive hormones and sperm quality with, the observed perturbation of male reproductive hormonal balance and poor sperm quality after HAART and anti-Koch's treatment agrees with previous data. Studies of Shayakhmetova et al (2015), Shayakhmetova et al (2017) observed that isoniazid, a component of anti-Koch's cocktail induced impaired spermatogenesis and sperm DNA damage. Studies on isoniazid and streptomycin also revealed that these drugs when co-administered significantly alter sperm morphology (Alp et al, 2012).…”

“…On the other hand, studies have reported that the administration of anti-Koch's cocktail containing ethambutol, isoniazid, rifampicin and pyrazinamide triggers oxidative testicular and sperm damage (Shayakhmetova et al, 2012). Anti-Koch's-induced reproductive toxicity has been linked with induction of testicular cytochrome P-450 2EI mRNA expression which triggers generation of reactive oxygen species and other toxic metabolites that mediates DNA damage, impaired spermatogenesis and reduced male fertility (Shayakhmetova et al, 2015(Shayakhmetova et al, , 2017. Although the efficacy of anti-Koch's therapy against Mycobacterium tuberculosis is undoubted, their side effects, particularly reproductive toxicity, have compromised its adherence.…”

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

“…And finally, it was a suppression of the xenobiotic‐metabolizing CYP expression by negative regulators such as inflammation. Previous studies reported that non‐steroidogenic P450s including CYP2E1 and CYP3A2 were present in rat testis . Tebuconazole dose‐dependently decreased testicular aniline hydroxylase (CYP2E1) and erythromycin N‐demethylase (CYP3A) activities, confirming the presence of these P450s in rat testis and further indicating their responsiveness to xenobiotic inhibitory effects.…”

High-energy diet enhances spermatogenic function and increases sperm midpiece length in fallow deer ( Dama dama ) yearlings

“…Moreover, it is worth noting that the nutritionally essential amino acids found in barley grain (i.e. lysine, methionine, cysteine and tryptophan; [19]) exert positive effects on spermatogenesis, sperm quality and fertility [20][21][22]. Taking into account that spermatogenesis in small domestic ruminants (i.e.…”