Comparative investigations of the effect of 5-aminolevulinate feeding on phytochrome and protochlorophyll(ide) content in dark-grown seedlings of barley, cucumber and cress

Schendel, Rudolf; Dörnemann, D.; Rüdiger, Wolfhart; Sineshchekov, V.A.

doi:10.1016/s1011-1344(96)07390-3

Cited by 11 publications

(4 citation statements)

References 25 publications

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“…First, a laboratory‐designed instrument based on two double‐grating monochromators of the DFS types (diffraction spectrometers, LOMO, Leningrad, USSR) conventionally used for the Raman spectroscopy was employed . Second, a spectrofluorimeter (Shimadzu RF‐5000; Shimadzu, Tokyo, Japan) equipped with a laboratory‐designed application for sample cooling as in our earlier measurements . It consisted of a bifurcated light guide, of which one branch was used for conducting exciting light to the sample and the second branch for directing the emission light from the sample to the entrance aperture of the emission monochromator of the spectrofluorimeter.…”

Section: Methodsmentioning

confidence: 99%

Protein Phosphatase Activity and Acidic/Alkaline Balance as Factors Regulating the State of Phytochrome A and its Two Native Pools in the Plant Cell

Sineshchekov

Koppel

Shor

et al. 2012

Photochem & Photobiology

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Phytochrome A (phyA), the most versatile plant phytochrome, exists in the two isoforms, phyA' and phyA'', differing by the character of its posttranslational modification, possibly, by phosphorylation at the N-terminal extension [Sineshchekov, V. (2010) J. Botany 2010, Article ID 358372]. This heterogeneity may explain the diverse modes of phyA action. We investigated possible roles of protein phosphatases activity and pH in regulation of the phyA pools' content in etiolated seedlings of maize and their extracts using fluorescence spectroscopy and photochemistry of the pigment. The phyA'/phyA'' ratio varied depending on the state of development of seedlings and the plant tissue/organ used. This ratio qualitatively correlated with the pH in maize root tips. In extracts, it reached a maximum at pH ≈ 7.5 characteristic for the cell cytoplasm. Inhibition of phosphatases of the PP1 and PP2A types with okadaic and cantharidic acids brought about phyA' decline and/or concomitant increase of phyA'' in coleoptiles and mesocotyls, but had no effect in roots, revealing a tissue/organ specificity. Thus, pH and phosphorylation status regulate the phyA'/phyA'' equilibrium and content in the etiolated (maize) cells and this regulation is connected with alteration of the processes of phyA' destruction and/or its transformation into the more stable phyA''.

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Section: Methodsmentioning

confidence: 99%

Protein Phosphatase Activity and Acidic/Alkaline Balance as Factors Regulating the State of Phytochrome A and its Two Native Pools in the Plant Cell

Sineshchekov

Koppel

Shor

et al. 2012

Photochem & Photobiology

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“…The negative effect is not connected with the availability of the chromophore, because the free chromophore, Pchlide 633 , experienced an increase under FRc. Also, feeding of etiolated seedlings with the Pchlide precursor, δ-aminolevulinic acid, increased the Pchlide 633 content by 10-100-fold while the content of Pchlide 655 remained practically unchanged [85], suggesting that the limiting factor for the Pchlide 655 formation is the apoprotein.…”

Section: Functional Distinctions Betweenmentioning

confidence: 97%

Fluorescence and Photochemical Investigations of Phytochrome in Higher Plants

Sineshchekov

2010

Journal of Botany

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In higher plants, photoreceptor phytochrome (phy)—photoisomerizing biliprotein working as a light-driven molecular switch—is represented by a small family of phytochrome gene products with phyA and phyB as major species. phyA is unique among other phytochromes mediating photoresponse modes specific only for this pigment (far-red light induced) and also photoresponses characteristic of phyB and other minor phys (red light induced). In our group, in vivo fluorescence investigations of phytochrome were initiated and two native phyA pools—posttranslationally modified PHYA gene products designated phyA′ and phyA″—were detected in dicots and monocots. They differ by spectroscopic and photochemical parameters, by abundance and distribution in etiolated plant tissues, by light stability, and other phenomenological characteristics, and, most importantly, by their functional properties. This may explain, at least partially, the nature of the uniqueness of the phyA action. In this paper, the data on the phyA polymorphism are summarized with attention to the applied experimental approach.

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“…In our study, we found similar concentrations of Pchlide in cotyledons of control samples. Several authors have proven that exogenous application of ALA increases the accumulation of Pchlide in etioplasts (Granick 1959, Wolwertz and Brouers 1980, Schendel et al 1996, Domanskii et al 2003). In our study, Pchlide concentration increased after 24 h incubation in ALA10 by 83.4% and 67.3% in L. decidua and in P. abies Fig.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of photosynthetic pigments in Larix decidua Mill. and Picea abies (L.) Karst. cotyledons treated with 5-aminolevulinic acid under different irradiation

Maximova¹,

Slováková²

2014

Photosynt.

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European larch (Larix decidua Mill.) and Norway spruce [Picea abies (L.) Karst.] synthesize chlorophyll (Chl) in darkness. This paper compares Chl accumulation in 14-d-old dark-grown seedlings of L. decidua and P. abies after shortterm (24 h) feeding with 5-aminolevulinic acid (ALA). We used two ALA concentrations (1 and 10 mM) fed to cotyledons of both species in darkness and in continuous light. The dark-grown seedlings of L. decidua accumulated Chl only in trace amounts and the seedlings remained etiolated. In contrast, P. abies seedlings grown in darkness were green and had significantly higher Chl content. After ALA feeding, higher protochlorophyllide (Pchlide) content was observed in L. decidua than in P. abies cotyledons incubated in darkness. Although short-term ALA feeding stimulated the synthesis of Pchlide, Chl content did not change significantly in cotyledons incubated in darkness. The Chl accumulation in cotyledons fed with ALA was similar to the rate of Chl accumulation in the controls. Higher Chl accumulation was reported in control samples after illumination: 86.9% in L. decidua cotyledons and 46.4% in P. abies cotyledons. The Chl content decreased and bleaching occurred in cotyledons incubated with ALA in light due to photooxidation. Analyses of Chlbinding proteins (D1 and LHCIIb) by Western blotting proved differences between Chl biosynthesis in L. decidua and P. abies seedlings in the dark and in the light. No remarkable increase was found in protein accumulation (D1 and LHCIIb) after ALA application. Our results showed interspecific difference in Chl synthesis between two gymnosperms. Shortterm ALA feeding did not stimulate Chl synthesis, thus ALA synthesis was not the rate-limiting step in Chl synthesis in the dark.

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Comparative investigations of the effect of 5-aminolevulinate feeding on phytochrome and protochlorophyll(ide) content in dark-grown seedlings of barley, cucumber and cress

Cited by 11 publications

References 25 publications

Protein Phosphatase Activity and Acidic/Alkaline Balance as Factors Regulating the State of Phytochrome A and its Two Native Pools in the Plant Cell

Protein Phosphatase Activity and Acidic/Alkaline Balance as Factors Regulating the State of Phytochrome A and its Two Native Pools in the Plant Cell

Fluorescence and Photochemical Investigations of Phytochrome in Higher Plants

Accumulation of photosynthetic pigments in Larix decidua Mill. and Picea abies (L.) Karst. cotyledons treated with 5-aminolevulinic acid under different irradiation

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