Comparative Investigations of Various Immunoregulatory Substances in the Delayed Type Hypersensitivity Test of the Mouse

Abstract

The substances D-penicillamine, auranofin, chloroquine, levamisole, BM 41.332, azimexone, bestatin, methisoprinol (inosiplex), thymosine (fraction 5), indomethacin and cyclophosphamide were examined comparatively in the delayed type hypersensitivity test after oxazolone sensitisation in mice. It was found, that only the basal antirheumatic drugs D-penicillamine, auranofin, chloroquine and levamisole and also BM 41.332 led to a potentiation of the DTH reactions. Methisoprinol, bestatin, azimexone, thymosine fraction 5 and indomethacin had no effect on the DTH, whilst the immunosuppressant cyclophosphamide led to an inhibition of the DTH reaction. It is concluded that this pharmacological model is suitable for screening of new basal drugs for rheumatoid arthritis.

Studies on type II collagen induced arthritis in mice

“…The inhibition seen in the developing disease may be due to the lowering of the circulating levels of antibodies coupled with the compounds known anti-inflammatory activity [15,22,23] which probably depends on a eytotoxic effect on bone marrow derived inflammatory cells. The inhibition of the established lesions may have been due to the compounds ability to suppress cell mediated reactions [24].…”

The Antitumor Effect of Bleomycin Combined with Bestatin against Ehrlich Ascites Carcinoma in Mice

“…Bestatin, one of the agents that serves this purpose, is being examined as an immunomodulator for cancer treatment. Bestatin is suggested to stimulate the immune system mainly by activating macrophages (8) and also to stimulate T-lymphocyte responses as measured by delayed-type hypersensitivity (DTH) testing ( 17). In previous studies, bestatin stimulated the differentiation of bone marrow gtanulocyte stem cells in a medium containing colony-stimulating factor (1 1) and promoted the production of interleukin-1 and interleukin-2.…”