Comparative isoenzyme electrophoreses between the brown-spotted locust, Cyrtacanthacris tatarica, and the desert locust, Schistocerca gregaria

Abstract

The desert locust, Schistocerca gregaria (Forskål) (Orthoptera: Acrididae), and the brown-spotted locust, Cyrtacanthacris tatarica (Linné) (Orthoptera: Acrididae), were collected from Saudi Arabia to investigate their relationships . Native polyacrylamide gel electrophoreses of five arbitrarily chosen metabolic enzymes extracted from the leg muscles of the two locust taxa were conducted. These enzymes were acid phosphatase ( Acph ), alcohol dehydrogenase ( Adh ), βester-ase ( β est ), malic enzyme ( Mal) and malate dehydrogenase ( Mdh ). Twenty presumptive gene loci and 26 polymorphic alleles were recorded. Acph did not discriminate between the two locust species, while the other four isoenzymes discriminated between them. Most of the alleles were monomeric, but Mal and Mdh exhibited dimeric alleles in the samples of C. tatarica . βest frac-tions were more expressed in C. tatarica , and the three enzymes βest , Mal , and Mdh discriminated clearly between the two species. The similarity coefficient that was calculated according to the number of sharing alleles between the two locusts was found to be 0.69. The isoenzyme variation presented herein seemed to reflect either their physiological adaptation or the taxonomic consequences between the two taxa. Collecting more isoenzymes for more sam-ples could have taxonomic value.

Toxicity of Some Essential Oils against Myiasis-Producing Fly, Megaselia scalaris, and their Impacts on Proteins and Detoxification Enzymes.

“…Any set of enzymes that are similar in catalytic properties and occurring in different tissues in a single species but are differentiated by variations in physical properties, such as isoelectric point, was called isoenzyme (isozymes). Isozymes estimation is considered as a good biochemical marker to differentiate between different species and treatments (Elsayed and Amer, 2014). Determination of isozymes has an important role to evaluate the possible effects of tested essential oils treatment against the 3 rd instar larvae of M. scalaris.…”

Characterization of the complete mitogenome of brown-spotted locust, Cyrtacanthacris tatarica (Orthoptera: Acrididae: Cyrtacanthacridinae)

“…Cyrtacanthacris tatarica (Linnaeus, 1758) belongs to the genus Cyrtacanthacris (Orthoptera: Cyrtacanthacridinae), which is considered as a potential pest for the scattered vegetation of herbs and shrubs (Elsayed et al. 2014 ). To data, no species within the genus is known for the mitogenome sequence.…”