Comparative iTRAQ-based proteomic analysis provides insight into a complex regulatory network of Pogostemon cablin in response to exogenous MeJA and Ethrel

Li, Junren; Chen, Xiuzhen; Zhong, Liting; Wang, Xiaobing; Zhou, Xiuping; Tang, Yun; Liu, Yanting; Hai, Zheng; Zhan, Renya; Chen, Likai

doi:10.1016/j.indcrop.2019.111661

Cited by 15 publications

(10 citation statements)

References 88 publications

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“…9). Both endogenous JAs and MeJA in Huangguogan and Shiranuhi were inhibited by exogenous MeJA in a manner that was dependent on treatment time, which is consistent with the trend in Pogostemon cablin (Li et al 2019). To further clarify why the endogenous JAs decreased, the key substances and enzymes of JAs synthesis were analyzed.…”

Section: Discussionsupporting

confidence: 83%

“…However, as research findings accumulate, it is inevitable that contrasting hypotheses will emerge. Research in Pogostemon cablin (Li et al 2019) supported the negative feedback regulation of exogenous signals. Meanwhile, the results of transcriptome studies in Arabidopsis (Lin et al 2019) and bilberry (Benevenuto et al 2019) plants showed that no significant upregulation of JA‐synthesizing genes was found in plants when an exogenous MeJA signal was actively given.…”

Section: Introductionmentioning

confidence: 96%

“…A study in cauliflower revealed that the addition of MeJA could improve photosynthetic rates and antioxidant activities (Wu et al 2012). Moreover, a study in Pogostemon cablin (Li et al 2019) demonstrated that extrinsic MeJA can promote the upregulation of photosynthesis‐related proteins. Additional studies have also shown that the use of JAs can impair photosynthetic systems but can enhance the ability of plants to cope with external pressure during stress by increasing photosynthesis (Quan et al 2018, Bali et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Effects of exogenous methyl jasmonate on the synthesis of endogenous jasmonates and the regulation of photosynthesis in citrus

Qiu

Xiong

et al. 2020

Physiologia Plantarum

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Methyl jasmonate (MeJA) is an airborne signaling phytohormone that can induce changes in endogenous jasmonates (JAs) and cause photosynthetic responses. However, the response of these two aspects of citrus plants at different MeJA concentrations is still unclear. Four MeJA concentrations were used in two citrus varieties, Huangguogan (C. reticulata × C. sinensis) and Shiranuhi [C. reticulata × (C. reticulata × C. sinensis)], to investigate the effects of MeJA dose on the endogenous JAs pathway and photosynthetic capacity. We observed that MeJA acted in a dosedependent manner, and its stimulation in citrus leaves showed a bidirectional character at different concentrations. This work demonstrates that MeJA at only a concentration of 2.2 mM or less contributed to the activation of magnesium protoporphyrin IX methyltransferase (ChlM, EC 2.1.1.11) and protochlorophyllide oxidoreductase (POR, EC 1.3.1.11) and the simultaneous accumulation of Chl a and Chl b, which in turn contributed to an improved photosynthetic capacity and PSII photochemistry efficiency of citrus. Meanwhile, the inhibition of endogenous JAs synthesis by exogenous MeJA was observed. This was achieved by reducing the ratio of monogalactosyl diacylglycerol (MGDG) to diagalactosyl diacylglycerol (DGDG) and inhibiting the activities of key enzymes in JAs synthesis, especially 12-oxo-phytodienoic acid reductase (OPR, EC 1.3.1.42). Another noteworthy finding is that there may exist a JA-independent pathway that could regulate 12-oxo-phytodienoic acid (OPDA) synthesis. This study jointly analyzed the internal hormone regulation mechanism and the external physiological response, as well as revealed the effects of exogenous MeJA on promoting the photosynthesis and inhibiting the endogenous JAs synthesis.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Effects of exogenous methyl jasmonate on the synthesis of endogenous jasmonates and the regulation of photosynthesis in citrus

Qiu

Xiong

et al. 2020

Physiologia Plantarum

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“…In addition, α-linolenic acid, which can attenuate a variety of inflammatory reactions, is typically consumed as part of a dietary supplement 68 . Reportedly, LOX as a major substrate takes part in the α-linolenic acid metabolic pathway and can catalyze linoleate oxidation in higher plants 69 . In the present study, we showed that six LOX-encoded genes were differentially expressed.…”

Section: Discussionmentioning

confidence: 99%

Integrative iTRAQ-based proteomic and transcriptomic analysis reveals the accumulation patterns of key metabolites associated with oil quality during seed ripening of Camellia oleifera

Yan

et al. 2021

Hortic Res

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Camellia oleifera (C. oleifera) is one of the four major woody oil-bearing crops in the world and has relatively high ecological, economic, and medicinal value. Its seeds undergo a series of complex physiological and biochemical changes during ripening, which is mainly manifested as the accumulation and transformation of certain metabolites closely related to oil quality, especially flavonoids and fatty acids. To obtain new insights into the underlying molecular mechanisms, a parallel analysis of the transcriptome and proteome profiles of C. oleifera seeds at different maturity levels was conducted using RNA sequencing (RNA-seq) and isobaric tags for relative and absolute quantification (iTRAQ) complemented with gas chromatography-mass spectrometry (GC-MS) data. A total of 16,530 transcripts and 1228 proteins were recognized with significant differential abundances in pairwise comparisons of samples at various developmental stages. Among these, 317 were coexpressed with a poor correlation, and most were involved in metabolic processes, including fatty acid metabolism, α-linolenic acid metabolism, and glutathione metabolism. In addition, the content of total flavonoids decreased gradually with seed maturity, and the levels of fatty acids generally peaked at the fat accumulation stage; these results basically agreed with the regulation patterns of genes or proteins in the corresponding pathways. The expression levels of proteins annotated as upstream candidates of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) as well as their cognate transcripts were positively correlated with the variation in the flavonoid content, while shikimate O-hydroxycinnamoyltransferase (HCT)-encoding genes had the opposite pattern. The increase in the abundance of proteins and mRNAs corresponding to alcohol dehydrogenase (ADH) was associated with a reduction in linoleic acid synthesis. Using weighted gene coexpression network analysis (WGCNA), we further identified six unique modules related to flavonoid, oil, and fatty acid anabolism that contained hub genes or proteins similar to transcription factors (TFs), such as MADS intervening keratin-like and C-terminal (MIKC_MADS), type-B authentic response regulator (ARR-B), and basic helix-loop-helix (bHLH). Finally, based on the known metabolic pathways and WGCNA combined with the correlation analysis, five coexpressed transcripts and proteins composed of cinnamyl-alcohol dehydrogenases (CADs), caffeic acid 3-O-methyltransferase (COMT), flavonol synthase (FLS), and 4-coumarate: CoA ligase (4CL) were screened out. With this exploratory multiomics dataset, our results presented a dynamic picture regarding the maturation process of C. oleifera seeds on Hainan Island, not only revealing the temporal specific expression of key candidate genes and proteins but also providing a scientific basis for the genetic improvement of this tree species.

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“…In cultivation and production, the quality of patchouli is often related to planting light conditions, ambient temperature and different abiotic and biotic stresses. Previous studies in our research group found that these factors affected the expression of the patchouli alcohol synthase gene and caused significant differences in patchouli alcohol accumulation [6]. Subsequently, various exogenous hormone treatment experiments showed that patchouli alcohol synthesis was specifically induced by Methyl jasmonate (MeJA).…”

Section: Introductionmentioning

confidence: 99%

PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin

Wang

Chen

Zhong

et al. 2019

IJMS

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The JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators in the jasmonic acid (JA) signaling pathways of plants, and these proteins have been reported to play key roles in plant secondary metabolism mediated by JA. In this study, we firstly isolated one JAZ from P. cablin, PatJAZ6, which was characterized and revealed based on multiple alignments and a phylogenic tree analysis. The result of subcellular localization indicated that the PatJAZ6 protein was located in the nucleus of plant protoplasts. The expression level of PatJAZ6 was significantly induced by the methyl jasmonate (MeJA). Furthermore, by means of yeast two-hybrid screening, we identified two transcription factors that interact with the PatJAZ6, the PatMYC2b1 and PatMYC2b2. Virus-induced gene silencing (VIGS) of PatJAZ6 caused a decrease in expression abundance, resulting in a significant increase in the accumulation of patchouli alcohol. Moreover, we overexpressed PatJAZ6 in P. cablin, which down-regulated the patchoulol synthase expression, and then suppressed the biosynthesis of patchouli alcohol. The results demonstrate that PatJAZ6 probably acts as a repressor in the regulation of patchouli alcohol biosynthesis, contributed to a model proposed for the potential JA signaling pathway in P. cablin.

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Comparative iTRAQ-based proteomic analysis provides insight into a complex regulatory network of Pogostemon cablin in response to exogenous MeJA and Ethrel

Cited by 15 publications

References 88 publications

Effects of exogenous methyl jasmonate on the synthesis of endogenous jasmonates and the regulation of photosynthesis in citrus

Effects of exogenous methyl jasmonate on the synthesis of endogenous jasmonates and the regulation of photosynthesis in citrus

Integrative iTRAQ-based proteomic and transcriptomic analysis reveals the accumulation patterns of key metabolites associated with oil quality during seed ripening of Camellia oleifera

PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin

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