Comparative karyotype study of three Cyprinids (Cyprinidae, Cyprininae) in Thailand by classical cytogenetic and FISH techniques

Abstract

Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, Epalzeorhynchos frenatum (Fowler, 1934), Puntigrus partipentazona (Fowler, 1934), Scaphognathops bandanensis Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent in situ hybridization (FISH) techniques. Chromosomes were directly prepared from kidney tissues and stained by using conventional and Ag-NOR banding techniques. Microsatellite d(CA)15 and d(CGG)10 probes were hybridized to the chromosomes of three cyprinids. The results show that the three cyprinid species share the same diploid number as 2n=50 but there are differences in the fundamental number (NF) and karyotypes i.e. E. frenatum: NF = 78, 18m+10sm+10st+12a; P. partipentazona: NF = 80, 6m+24sm+14st+6a; S. bandanensis: NF = 66, 4m+12sm+34a. NOR positive masks were observed at the regions adjacent to the telomere of the short arm of the chromosome pairs 10 (submetacentric) and 1 (metacentric) in E. frenatum and P. partipentazona, respectively whereas those were revealed at telomeric regions of the long arm of the chromosome pair 9 (acrocentric) in S. bandanensis. The mapping of d(CA)15 and d(CGG)10 microsatellites shown that hybridization signals are abundantly distributed in telomeric regions of several pairs except d(CA)15 repeats in S. bandanensis, which are distributed throughout all chromosomes and d(CGG)10 repeats in P. partipentazona display the high accumulation only in the first chromosome pair.

A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand

“…Besides), the occurrence of chromosomal rearrangements has been considered a relatively common evolutionary mechanism inside the Cyprinidae family reviewed (Arai 2011). Family Cyprinidae are diploid chromosome ranges from 48-50 in the tribes Labeonini and Smiliogastrini while the tribe Poropuntiini and Danionini are more conserved as 2n = 50 (Phimphan et al 2020).…”

Adding New Pieces to the Puzzle of Karyotype Evolution in Harttia (Siluriformes, Loricariidae): Investigation of Amazonian Species

“…The 18S rDNA probe, containing a 1400 bp-long segment of the transcribed region was also isolated from H. malabaricus following Cioffi et al [ 43 ] and green-labeled with AF488-dUTP using the Nick-Translation mix kit (Jena Bioscience). The microsatellites (A) 30 , (CA) 15 , and (GA) 15 were selected based on their abundance and patterns of chromosome distribution encountered in previous studies in different fish species [ 36 , 44 ], and the corresponding probes were directly labeled with Cy3 (Sigma-Aldrich, Darmstadt, Germany) during their synthesis, as described in [ 45 ]. Telomeric sequence (TTAGGG) n was in situ located using the DAKO Telomere PNA FISH Kit/FITC (DAKO, Glostrup, Denmark).…”

Karyomorphological delineation, and the NOR loci on the sex chromosome in three species of Chrysopeleinid (Chrysopeleinae: Colubridae) from Thailand

“…Conventional staining was done using 20% Giemsa's stock solution for 30 min (Patawang et al 2014). 50% AgNO3 and 2% gelatin were used for Ag-NOR banding technique (Patawanget al 2017;Sangpakdee et al 2017;Phimphan et al 2020;Thongnetr et al 2021).…”