Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities

Abstract

Lantana camara L. and Lantana montevidensis Briq. (F. Verbenaceae) are invasive ornamental weeds native to the tropical regions of Africa and America. The leaves of both species have been traditionally used as infusions for treating fever, rheumatism, and cancer. LC–MS–MS-guided profiling of the methanolic extracts of the leaves of L. camara and L. montevidensis growing in Egypt led to the putative identification of 59 compounds belonging to terpenoids, flavonoids, iridoid glycosides, phenolic acids, and their derivatives. The in-vitro antioxidants and anti-inflammatory and anticancer activities of the two extracts were investigated. L. camara and L. montevidensis inhibited DPPH• (IC50 = 34.01 ± 1.32 and 47.43 ± 1.74 µg/mL), ABTS+ (IC50 = 30.73 ± 1.42 and 40.37 ± 1.51 µg/mL), and superoxide anion (IC50 = 1.57 ± 0.19 and 1.31 ± 0.14 μg/mL) free radicals. A potent anti-inflammatory effect was observed for both species through the inhibition of elastase release in fMLF/CB-induced human neutrophils (IC50 = 2.40 ± 0.16 and 1.90 ± 0.07 μg/mL). The extracts showed significant cytotoxic activity against a panel of cancer cell lines with the most potent activity against Caco cells (IC50 = 45.65 ± 1.64 and 40.67 ± 1.52 µg/mL for L. camara and L. montevidensis, respectively). Western blotting supported by FACS analysis revealed that the extracts inhibited cancer cell proliferation, reduced metastasis, and induced apoptosis resulting in cell cycle arrest. This was achieved via increasing mRNA and protein expressions of p53 and GSK-3β as well as decreasing the expression of PI3K, Akt, and cyclin D1.

New Pyrazolothiazole as Potential Wnt/β‐Catenin Inhibitors: Green Synthesis, Characterization, Antimicrobial, Antioxidant, Antineoplastic Evaluation, and Molecular Docking Study

“…According to El‐Din et al.′s method, [42] the hydrogen atom or electron‐donating ability of the newly synthesized compounds was determined by the reduction of the purple color intensity of DPPH. From the following equation, the radical scavenging activity was determined: $$$\vcenter{\openup.5em\halign{$\displaystyle{#}$\cr {\bf \char37 }{\rm \ }{\bf D P P H}{\rm \ }{\bf s c a v e n g i n g}{\rm \ }{\bf a c t i v i t y}={{{\bf A}\left({\bf b l a n k}\right)-{\bf A}\left({\bf s a m p l e}\right)}\over{{\bf A}\left({\bf b l a n k}\right)}}{\rm \ }{\bf X}100\hfill\cr}}$$$ …”

“…According to El-Din et al's method, [42] the hydrogen atom or electron-donating ability of the newly synthesized compounds was determined by the reduction of the purple color intensity of DPPH.…”

Synthesis, molecular docking, and in-vitro studies of pyrimidine-2-thione derivatives as antineoplastic agents via potential RAS/PI3K/Akt/JNK inhibition in breast carcinoma cells

“…In a six-well plate, 1 × 10 5 MCF-7 cells were seeded and incubated for 24 h then treated with the IC 50 dose of the selected compound. Using an inverted light microscope (Olympus, USA), the morphological alterations of the treated and untreated cells were evaluated and documented after 48 h of incubation 34 .…”

UPLC-MS/MS Analysis of Naturally Derived Apis mellifera Products and Their Promising Effects against Cadmium-Induced Adverse Effects in Female Rats

“…The raw data files were converted to mzXML format using MSConvert from the ProteoWizard suite ( , accessed on 25 December 2022). The spectra in the network were then searched against Global Natural Products Social Molecular Networking (GNPS) spectra libraries and published data [ 35 , 36 ].…”