Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection.

Hiraishi, Akira; Ueda, Yôko; Ishihara, Junko; Mori, Tadahiro

doi:10.2323/jgam.42.457

Cited by 735 publications

(385 citation statements)

References 21 publications

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“…Isoprenoid quinones were extracted with chloroform/methanol (2 : 1, v/v), evaporated under vacuum conditions and re-extracted in n-hexane/water (1 : 1, v/v). The crude n-hexane-quinone solution was purified using Sep-Pak Vac silica cartridges (Waters) and subsequently analysed using HPLC, as described previously (Hiraishi et al, 1996). Cellular fatty acid profiles were determined for strains grown on R2A agar (Difco) for 2 days at 30 u C. The cellular fatty acids were saponified, methylated and extracted according to the protocol of the Sherlock Microbial Identification System (MIDI).…”

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confidence: 99%

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Ten

Baek

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A Gram-positive, non-motile, endospore-forming bacterial strain, designated Gsoil 420T, was isolated from soil of a ginseng field in Pocheon Province, South Korea, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The novel isolate consisted of strictly aerobic, rod-shaped cells and was able to grow in medium supplemented with up to 12 % NaCl at 25 °C and pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 420T fell within the radiation of the cluster comprising Bacillus species and formed a coherent cluster with Bacillus niacini (16S rRNA gene sequence similarity, 98.6 %), Bacillus bataviensis (98.6 %), Bacillus soli (98.3 %), Bacillus drentensis (98.0 %), Bacillus novalis (98.0 %), Bacillus vireti (97.9 %), Bacillus foraminis (97.6 %), Bacillus fumarioli (97.4 %) and Bacillus jeotgali (97.0 %). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.8 %. Strain Gsoil 420T had a genomic DNA G+C content of 44.9 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were anteiso-C15 : 0 (33.9 %), iso-C15 : 0 (24.5 %) and iso-C14 : 0 (19.9 %). These chemotaxonomic results supported the affiliation of strain Gsoil 420T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Gsoil 420T from recognized Bacillus species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 420T represents a novel species of the genus Bacillus, for which the name Bacillus pocheonensis sp. nov. is proposed. The type strain is Gsoil 420T (=KCTC 13943T=DSM 18135T).

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confidence: 99%

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Ten

Baek

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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“…The crude n-hexane-quinone solution was purified using Sep-Pak Vac cartridges silica (Waters) and subsequently analyzed by HPLC as previously described (Hiraishi et al, 1996). Cellular fatty acids profiles were determined for strains grown on R2A agar for 2 days at 30 C. The cellular fatty acids were saponified, methylated and extracted according to the protocol of the Sherlock Microbial Identification System (MIDI).…”

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Niabella aquatica sp. nov., isolated from lake water

Ṣiddiqi

2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-reaction-negative, strictly aerobic, non-motile, yellow and rod-shaped bacterium (designated RP-2 T ) isolated from lake water, was characterized by a polyphasic approach to clarify its taxonomic position. Strain RP-2 T was observed to grow optimally at 30 C and at pH 7.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that RP-2 T represented a member of the genus Niabella of the family Chitinophagaceae and was most closely related to Niabella yanshanensis KACC 14980 T (96.6 %), Niabella ginsengisoli KACC 13021 T (96.5 %), Niabella drilacis DSM 25811 T (95.7 %) and Niabella aurantiaca KACC 11698 T (95.6 %). The DNA G+C content was 44.5 mol%. The major polar lipids were diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The predominant quinone was MK-7. The major fatty acids were iso-C 15:0 , iso-C 15:1 G, iso-C 17:0 3-OH and summed feature 3 (comprising C 16:1 !7c and/or C 16:1 !6c) supported the affiliation of RP-2 T to the genus Niabella. However, RP-2 T could be distinguished genotypically and phenotypically from the species of the genus Niabella with validly published names. The novel isolate therefore represents a novel species, for which the name Niabella aquatica sp. nov. is proposed, with the type strain RP-2 T (=KACC 18623 T =JCM 30952 T ).

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“…After evaporating solvent under vacuum conditions, isoprenoid quinones were re-extracted using n-hexane. Crude quinones dissolved in n-hexane were purified using Sep-Pak Vac silica cartridges (Waters) and identified by HPLC (Hiraishi et al, 1996). Cellular fatty acids were analysed using cell biomass grown on R2A at 30 u C for 5 days.…”

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confidence: 99%

Luteolibacter yonseiensis sp. nov., isolated from activated sludge using algal metabolites

Park

Baek

Woo

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, rod-shaped, aerobic bacterial strain, designated EBTL01 T , was isolated from activated sludge by using metabolites of microalgae Ankistrodesmus gracilis SAG278-2. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain EBTL01 T belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is related most closely to Luteolibacter pohnpeiensis A4T-83 T (95.5 % sequence similarity) and Luteolibacter algae A5J-41-2 T (95.2 %). The G+C content of the genomic DNA of strain EBTL01 T was 56.3 mol% and the menaquinone MK-9 was detected as the predominant quinone. Major fatty acid components were iso-C 14 : 0 , C 16 : 1 v7c and C 16 : 0 . The amino acids of the cell-wall peptidoglycan contained muramic acid and meso-diaminopimelic acid. These profile results supported the affiliation of strain EBTL01 T to the genus Luteolibacter. On the other hand, based on chemotaxonomic properties and phenotypic characteristics, strain EBTL01 T could be clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL01 T represents a novel species of the genus Luteolibacter, for which the name Luteolibacter yonseiensis sp. nov. is proposed. The type strain is EBTL01 T (5KCTC 23678 T 5JCM 18052 T ).The genus Luteolibacter, a member of the family Verrucomicrobiaceae, class Verrucomicrobiae, was proposed by Yoon et al. with the description of Luteolibacter pohnpeiensis A4T-83 T in 2008. At the time of writing, the genus Luteolibacter comprises two species with validly published names, Luteolibacter pohnpeiensis A4T-83 T and Luteolibacter algae A5J-41-2 T (Yoon et al., 2008). Members of the genus Luteolibacter have the following characteristics: they are Gram-negative, non-endospore-forming, nonmotile rods and positive for catalase and oxidase. They contain iso-C 14 : 0 as the predominant cellular fatty acid and the genomic DNA G+C contents of known strains range from 55.8 to 55.9 mol%. During the screening of bacterial strains from activated sludge of industrial wastewater treatment by using algal metabolites, a Gram-negative, rodshaped bacterial strain, designated EBTL01 T , was isolated. On the basis of 16S rRNA gene sequence analysis, this isolate was considered to be a Luteolibacter-like strain. To determine its exact taxonomic position, EBTL01 T was subjected to a detailed investigation using a polyphasic taxonomic approach, including genotypic, chemotaxonomic and classical phenotypic analyses. These results indicate that EBTL01 T should be placed in the genus Luteolibacter as a representative of a novel species. Algal metabolites were obtained by culturing microalgae Ankistrodesmus gracilis SAG278-2 in artificial wastewater for 2 weeks. The artificial wastewater contained following components (in mg l 21 ): NaCl (7), CaCl 2 (4), MgSO 4 . 7H 2 O (2), K 2 HPO 4 (21.7), KH 2 PO 4 (8.5), Na 2 HPO 4 (33.4) and NH 4 Cl (3). Algal metabolites agar media were prepared for isolating micro-organisms that can utilize algal metabolites as their carbon and nutrient sources. An activated s...

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Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection.

Cited by 735 publications

References 21 publications

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

Niabella aquatica sp. nov., isolated from lake water

Luteolibacter yonseiensis sp. nov., isolated from activated sludge using algal metabolites

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