Comparative mapping of twenty-eight  bovine loci in sheep (&lt;i&gt;Ovis aries&lt;/i&gt;, 2n = 54) and river buffalo (&lt;i&gt;Bubalus bubalis&lt;/i&gt;, 2n = 50) by FISH

Meo, G. P. Di; Perucatti, A.; Incarnato, D.; Ferretti, Luca; Berardino, D. Di; Jambrenghi, Anna Caputi; Vonghia, G.; Iannuzzi, L.

doi:10.1159/000071045

Cited by 13 publications

(3 citation statements)

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“…The genetics of livestock animals are fairly well known. The domestic sheep (Ovis aries), as one such livestock species, has been well characterized in terms of both genetics and cytogenetics (Echard et al 1994;Di Meo et al 2002, 2007. The diploid chromosome number of the species is 54.…”

Section: Introductionmentioning

confidence: 99%

Analysis of chromatin instability of somatic cells in sheep

et al. 2018

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Cytogenetic tests are highly reliable, sensitive indicators of the early effects of genomic instability. Their results provide information on the organism’s susceptibility to exogenous and endogenous factors and are measures of the degree of repair of DNA damage. Our study assessed spontaneously occurring damage in following four breeds of sheep: Polish Heath, Polish Lowland (Zelazna variety), Polish Blackhead, and Berrichon du Cher. Instability was identified using the following three different tests: a sister chromatid exchange (SCE) assay, identification of fragile sites, and the comet assay. The distribution of instabilities varied depending on the breed. The mean frequency of SCEs was 5.13 ± 1.58, whereas that of fragile sites was 3.30 ± 1.24. The mean level of DNA damage (% head DNA) was 96.52 ± 6.59. The most damage to genetic material was observed in the Berrichon du Cher sheep, and the least in the Polish Heath sheep. The tests used are reliable biomarkers of genome stability in animal breeds, as well as in individuals within breeds.

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Section: Introductionmentioning

confidence: 99%

Analysis of chromatin instability of somatic cells in sheep

et al. 2018

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“…These genes are annotated on VPA1, VPA5, VPA9, VPA2/VPA32 and VPA11 in the alpaca. Comparative Zoo-FISH studies between bovine and sheep genomes [64,65] and studies between camel and bovine genomes [3] provide support for synteny between these sheep and alpaca chromosomes. We identified SNPs in these chromosomes, but they were located at other loci.…”

Section: Candidate Regionsmentioning

confidence: 83%

Genome-Wide Association Study of Fiber Diameter in Alpacas

More,

Veli,

Cruz

et al. 2023

Animals

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The aim of this study was the identification of candidate genomic regions associated with fiber diameter in alpacas. DNA samples were collected from 1011 female Huacaya alpacas from two geographical Andean regions in Peru (Pasco and Puno), and three alpaca farms within each region. The samples were genotyped using an Affymetrix Custom Alpaca genotyping array containing 76,508 SNPs. After the quality controls, 960 samples and 51,742 SNPs were retained. Three association study methodologies were performed. The GWAS based on a linear model allowed us to identify 11 and 35 SNPs (−log10(p-values) > 4) using information on all alpacas and alpacas with extreme values of fiber diameter, respectively. The haplotype and marker analysis method allowed us to identify nine haplotypes with standardized haplotype heritability higher than six standard deviations. The selection signatures based on cross-population extended haplotype homozygosity (XP-EHH) allowed us to identify 180 SNPs with XP-EHH values greater than |3|. Four candidate regions with adjacent SNPs identified via two association methods of analysis are located on VPA6, VPA9, VPA29 and one chromosomally unassigned scaffold. This study represents the first analysis of alpaca whole genome association with fiber diameter, using a recently assembled alpaca SNP microarray.

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“…1998a) and have been previously used to build comparative maps between ruminants, pig, horse and humans (Schibler et al. 1998b; Di Meo et al. 2000; 2002; 2006; Iannuzzi et al.…”

Section: Methodsmentioning

confidence: 99%

An advanced sheep (Ovis aries, 2n = 54) cytogenetic map and assignment of 88 new autosomal loci by fluorescence in situ hybridization and R‐banding

et al. 2007

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SummaryPresented herein is an updated sheep cytogenetic map that contains 452 loci (291 type I and 161 type II) assigned to specific chromosome bands or regions on standard R-banded ideograms. This map, which significantly extends our knowledge of the physical organization of the ovine genome, includes new assignments for 88 autosomal loci, including 74 type I loci (known genes) and 14 type II loci (SSRs/microsatellite marker/STSs), by FISHmapping and R-banding. Comparison of the ovine map to the cattle and goat cytogenetic maps showed that common loci were located within homologous chromosomes and chromosome bands, confirming the high level of conservation of autosomes among ruminant species. Eleven loci that were FISH-mapped in sheep (B3GAT2, ASCC3, RARSL, BRD2, POLR1C, PPP2R5D, TNRC5, BAT2, BAT4, CDC5L and OLA-DRA) are unassigned in cattle and goat. Eleven other loci (D3S32, D1S86, BMS2621, SFXN5, D5S3, D5S68, CSKB1, D7S49, D9S15, D9S55 and D29S35) were assigned to specific ovine chromosome (OAR) bands but have only been assigned to chromosomes in cattle and goat.

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Comparative mapping of twenty-eight bovine loci in sheep (<i>Ovis aries</i>, 2n = 54) and river buffalo (<i>Bubalus bubalis</i>, 2n = 50) by FISH

Cited by 13 publications

References 10 publications

Analysis of chromatin instability of somatic cells in sheep

Analysis of chromatin instability of somatic cells in sheep

Genome-Wide Association Study of Fiber Diameter in Alpacas

An advanced sheep (Ovis aries, 2n = 54) cytogenetic map and assignment of 88 new autosomal loci by fluorescence in situ hybridization and R‐banding

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