“…First, the Drabkin’s solution was prepared from potassium ferricyanide, potassium cyanide, and sodium bicarbonate. Next, 20 microliters (μL) of blood was mixed with the 5.0 ml of Drabkin’s reagent and allowed to stand for 5 min to develop color ( Pintavirooj et al, 2021 ; Madani et al, 2015 ). The resulting intensity was measured against a known standard at 546 nm using a URIT-810 ® semiautomatic biochemistry analyzer (Thermo Corp., Cambridge, UK) ( Bashir and Derar, 2019 ).…”