Comparative metabolic profiling of Ophiocordyceps sinensis and its cultured mycelia using GC–MS

Zhang, Jianshuang; Yu, Hao; Li, Shaosong; Zhong, Xin; Wang, Haizhen; Liu, Xin

doi:10.1016/j.foodres.2020.109241

Cited by 31 publications

(25 citation statements)

References 43 publications

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“…The interpretation rates (R 2 X = 0.776 and 0.691, R 2 Y = 0.994 and 0.997 for the PQ and Del groups, respectively) and the prediction (Q 2 = 0.981 and 0.973 for the PQ and Del groups, respectively) showed that the OPLS-DA model has good fitness and predictability. Permutation tests with 200 iterations were performed to check the validity and avoid overfitting [ 29 ]. As shown in Figure 3 A,B, R 2 (blue) and Q 2 (red) values to the left were lower than the right points.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Regulation Effect and Potential Metabolic Biomarkers of Pre-Treated Delphinidin on Oxidative Damage Induced by Paraquat in A549 Cells

Huang

et al. 2022

Foods

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Delphinidin (Del) is an anthocyanin component with high in vitro antioxidant capacity. In this study, based on the screening of a cell model, gas chromatography-time of flight mass spectrometry (GC-TOF/MS) was used to evaluate the effect of Del pre-protection on the metabolite levels of intracellular oxidative stress induced by paraquat (PQ). According to the cytotoxicity and reactive oxygen species (ROS) responses of four lung cell lines to PQ induction, A549 cell was selected and treated with 100 μM PQ for 12 h to develop a cellular oxidative stress model. Compared with the PQ-induced group, the principal components of the Del pretreatment group had significant differences, but not significant with the control group, indicating that the antioxidant activity of Del can be correlated to the maintenance of metabolite levels. Del preconditioning protects lipid-related metabolic pathways from the disturbance induced by PQ. In addition, the levels of amino acid- and energy-related metabolites were significantly recovered. Del may also exert an antioxidant effect by regulating glucose metabolism. The optimal combinations of biomarkers in the PQ-treatment group and Del-pretreatment group were alanine-valine-urea and alanine-galactose-glucose. Cell metabolome data provided characteristic fingerprints associated with the antioxidant activity of Del.

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Section: Resultsmentioning

confidence: 99%

Metabolic Regulation Effect and Potential Metabolic Biomarkers of Pre-Treated Delphinidin on Oxidative Damage Induced by Paraquat in A549 Cells

Huang

et al. 2022

Foods

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“…Irrespective of the detection method used, significant differences were found between OSBSz and OSBSh. Metabolites with VIP > 1 and p < 0.05 were considered as differentially expressed metabolites in this study [ 31 , 32 ]. In previous studies, some important differential metabolites showed significant biological activity.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild Ophiocordyceps sinensis

et al. 2022

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The authors of this paper conducted a comparative metabolomic analysis of Ophiocordyceps sinensis (OS), providing the metabolic profiles of the stroma (OSBSz) and sclerotia (OSBSh) of OS by widely targeted metabolomics and untargeted metabolomics. The results showed that 778 and 1449 metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. The metabolites in OSBSz and OSBSh are significantly differentiated; 71 and 96 differentially expressed metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. This suggests that these 71 metabolites (riboflavine, tripdiolide, bromocriptine, lumichrome, tetrahymanol, citrostadienol, etc.) and 96 metabolites (sancycline, vignatic acid B, pirbuterol, rubrophen, epalrestat, etc.) are potential biomarkers. 4-Hydroxybenzaldehyde, arginine, and lumichrome were common differentially expressed metabolites. Using the widely targeted metabolomics approach, the key pathways identified that are involved in creating the differentiation between OSBSz and OSBSh may be nicotinate and nicotinamide metabolism, thiamine metabolism, riboflavin metabolism, glycine, serine, and threonine metabolism, and arginine biosynthesis. The differentially expressed metabolites identified using the untargeted metabolomics approach were mainly involved in arginine biosynthesis, terpenoid backbone biosynthesis, porphyrin and chlorophyll metabolism, and cysteine and methionine metabolism. The purpose of this research was to provide support for the assessment of the differences between the stroma and sclerotia, to furnish a material basis for the evaluation of the physical effects of OS, and to provide a reference for the selection of detection methods for the metabolomics of OS.

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“…This suggested that saccharides were enriched in the mycelia in order to serve as essential nutrients during fruiting body development. Other works also showed that differences were observed in the chemical composition and nutritional properties between natural caterpillar fungus and cultured mycelium of O. sinensis [50,51]. The start point of the metabolomics changing in O. sinensis fungus during its life stages is still elusive.…”

Section: Situation Dependent Metabolomics Of O Sinensismentioning

confidence: 99%

“…Using LC-MS, the metabolome differences between wild O. sinensis and artificial cultured C. militaris were conducted [53], and the components of aqueous extract of artificial cultured C. sinensis were profiled with focusing on the polar compounds [54]. Previously reported works also concluded that differences in metabolomics existed between mycelia and cordyceps [51]. A few works have focused on development or infection influences.…”

Section: Featured Metabolites and Their Potential Roles As New Quality Control Biomarkersmentioning

confidence: 99%

Stage- and Rearing-Dependent Metabolomics Profiling of Ophiocordyceps sinensis and Its Pipeline Products

Tang

Qiu

Cao

et al. 2021

Insects

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Cordyceps, a parasitic complex of the fungus Ophiocordyceps sinensis (Berk.) (Hypocreales: Ophiocordycipitaceae) and the ghost moth Thitarodes (Lepidoptera: Hepialidae), is a historical ethnopharmacological commodity in China. Recently, artificial cultivation of Chinese cordyceps has been established to supplement the dwindling natural resources. However, much is unknown between the natural and cultivated products in terms of nutritional aspect, which may provide essential information for quality evaluation. The current study aims to determine the metabolic profiles of 17 treatments from 3 sample groups including O. sinensis fungus, Thitarodes insect and cordyceps complex, using Gas Chromatography - Quadrupole Time-of-Flight Mass Spectrometry. A total of 98 metabolites were detected, with 90 of them varying in concentrations among groups. The tested groups could be separated, except that fungal fruiting body was clustered into the same group as Chinese cordyceps. The main distinguishing factors for the groups studied were the 24 metabolites involved in numerous different metabolic pathways. In conclusion, metabolomics of O. sinensis and its related products were determined mainly by the fruiting bodies other than culture methods. Our results suggest that artificially cultured fruiting bodies and cordyceps may share indistinguishable metabolic functions as the natural ones.

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Comparative metabolic profiling of Ophiocordyceps sinensis and its cultured mycelia using GC–MS

Cited by 31 publications

References 43 publications

Metabolic Regulation Effect and Potential Metabolic Biomarkers of Pre-Treated Delphinidin on Oxidative Damage Induced by Paraquat in A549 Cells

Metabolic Regulation Effect and Potential Metabolic Biomarkers of Pre-Treated Delphinidin on Oxidative Damage Induced by Paraquat in A549 Cells

Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild Ophiocordyceps sinensis

Stage- and Rearing-Dependent Metabolomics Profiling of Ophiocordyceps sinensis and Its Pipeline Products

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