Comparative Metabolome and Transcriptome Analysis of Anthocyanin Biosynthesis in White and Pink Petals of Cotton (Gossypium hirsutum L.)

Shao, Dongnan; Liang, Qian; Wang, Xuefeng; Zhu, Qian; Liu, Feng; Li, Yanjun; Zhang, Xinyu; YANG, Yong-lin; Sun, Jie; Xue, Fei

doi:10.3390/ijms231710137

Cited by 11 publications

(7 citation statements)

References 69 publications

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“…Notably, cyanidin-3-O-galactoside, peonidin-3,5-O-diglucoside, peonidin-3-O-(6’’-O-p-coumaroylglucoside)-5-O-glucoside, cyanidin-3-O-(2’’-O-xylosyl)galactoside, and cyanidin-3-O-sophorotrioside were either found or detected in significantly higher concentrations compared to the white petals. These findings suggest that these specific anthocyanins may play crucial roles in enhancing the deep purple color of PF petals ( Fu et al., 2021 ; Shao et al., 2022 ).…”

Section: Discussionmentioning

confidence: 87%

Transcriptome and metabolome analysis reveals the effect of flavonoids on flower color variation in Dendrobium nobile Lindl.

Qiu,

Cai,

et al. 2023

Front. Plant Sci.

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IntroductionDendrobium nobile L. is a rare orchid plant with high medicinal and ornamentalvalue, and extremely few genetic species resources are remaining in nature. In the normal purple flower population, a type of population material with a white flower variation phenotype has been discovered, and through pigment component determination, flavonoids were preliminarily found to be the main reason for the variation.MethodsThis study mainly explored the different genes and metabolites at different flowering stages and analysed the flower color variation mechanism through transcriptome- and flavonoid-targeted metabolomics. The experimental materials consisted of two different flower color phenotypes, purple flower (PF) and white flower (WF), observed during three different periods.Results and discussionThe results identified 1382, 2421 and 989 differentially expressed genes (DEGs) in the white flower variety compared with the purple flower variety at S1 (bud stage), S2 (chromogenic stage) and S3 (flowering stage), respectively. Among these, 27 genes enriched in the ko00941, ko00942, ko00943 and ko00944 pathways were screened as potential functional genes affecting flavonoid synthesis and flower color. Further analysis revealed that 15 genes are potential functional genes that lead to flavonoid changes and flower color variations. The metabolomics results at S3 found 129 differentially accumulated metabolites (DAMs), which included 8 anthocyanin metabolites, all of which (with the exception of delphinidin-3-o-(2’’’-o-malonyl) sophoroside-5-o-glucoside) were found at lower amounts in the WF variety compared with the PF variety, indicating that a decrease in the anthocyanin content was the main reason for the inability to form purple flowers. Therefore, the changes in 19 flavone and 62 flavonol metabolites were considered the main reasons for the formation of white flowers. In this study, valuable materials responsible for flower color variation in D. nobile were identified and further analyzed the main pathways and potential genes affecting changes in flavonoids and the flower color. This study provides a material basis and theoretical support for the hybridization and molecular-assisted breeding of D. nobile.

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Section: Discussionmentioning

confidence: 87%

Transcriptome and metabolome analysis reveals the effect of flavonoids on flower color variation in Dendrobium nobile Lindl.

Qiu,

Cai,

et al. 2023

Front. Plant Sci.

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“…This group of specialised plant metabolites includes > 10 000 different compounds. Plant GSTFs are involved in flavonoid biosynthesis (Shao et al ., 2022; Aktar et al ., 2022). Our understanding of the functions of almost all these substances remains poor, but there is increasing evidence to suggest a role for flavonoids in plant stress resistance (Sugiyama & Yazaki, 2014).…”

Section: Discussionmentioning

confidence: 99%

Meloidogyne enterolobiiMeMSP1 effector targets the glutathione‐S‐transferase phi GSTF family in Arabidopsis to manipulate host metabolism and promote nematode parasitism

Chen,

Liu,

Sun

et al. 2023

New Phytologist

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Summary Meloidogyne enterolobii is an emerging root‐knot nematode species that overcomes most of the nematode resistance genes in crops. Nematode effector proteins secreted in planta are key elements in the molecular dialogue of parasitism. Here, we show the MeMSP1 effector is secreted into giant cells and promotes M. enterolobii parasitism. Using co‐immunoprecipitation and bimolecular fluorescent complementation assays, we identified glutathione‐S‐transferase phi GSTFs as host targets of the MeMSP1 effector. This protein family plays important roles in plant responses to abiotic and biotic stresses. We demonstrate that MeMSP1 interacts with all Arabidopsis GSTF. Moreover, we confirmed that the N‐terminal region of AtGSTF9 is critical for its interaction, and atgstf9 mutant lines are more susceptible to root‐knot nematode infection. Combined transcriptome and metabolome analyses showed that MeMSP1 affects the metabolic pathways of Arabidopsis thaliana, resulting in the accumulation of amino acids, nucleic acids, and their metabolites, and organic acids and the downregulation of flavonoids. Our study has shed light on a novel effector mechanism that targets plant metabolism, reducing the production of plant defence‐related compounds while favouring the accumulation of metabolites beneficial to the nematode, and thereby promoting parasitism.

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“…Ultra-high performance liquid chromatography (UPLC) (ExionLCTM AD) and tandem mass spectrometry (MS/MS) (QTRAPR6500+) were used for data collection. The specific liquid phase and mass spectrometry conditions were conducted following the methods previously reported ( Shao et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome and metabolome analyses of anthocyanin biosynthesis in post-harvest fruits of a full red-type kiwifruit (Actinidia arguta) ‘Jinhongguan’

Ye,

Bai,

Zhang

et al. 2023

Front. Plant Sci.

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Anthocyanin is the main component of pigment in red-fleshed kiwifruit. ‘Jinhongguan’ is a new cultivar of Actinidia arguta with red peel and flesh after harvest. However, the specific types of anthocyanin in the ‘Jinhongguan’ fruit and its biosynthesis pathways remain largely unknown. Here, the total anthocyanin content in the fruit color conversion process was determined. The results showed that total anthocyanin content increased with the deepening color of the peel and flesh. To identify the genes related to anthocyanin biosynthesis and the types of anthocyanins in the ‘Jinhongguan’ fruit, a combined analysis of transcriptome and anthocyanin-targeted metabolome was carried out. A total of 5751 common differentially expressed genes (DEGs) at different stages of peel and flesh were identified, of which 2767 were common up-DEGs and 2976 were common down-DEGs. KEGG and GO enrichment analyses showed that the common up-DEGs were significantly enriched in anthocyanin synthesis-related pathways, suggesting some up-DEGs are involved in anthocyanin biosynthesis. In total, 29 metabolites were detected in the flesh by anthocyanin-targeted metabolome. Among these, nine were differential accumulation metabolites (DAMs) in comparison to red flesh vs green flesh. Six DAMs were up-regulated, with five of them were cyanidins. The content of cyanidin-3-O-galactoside was much higher than that of other DAMs, making it the main pigment in ‘Jinhongguan’. Moreover, a total of 36 anthocyanin synthesis-related structural genes, 27 MYB transcription factors (TFs), 37 bHLH TFs and 9 WDR TFs were screened from the common DEGs. Correlation analysis of transcriptome and metabolome revealed that 9 structural genes, 6 MYB TFs, 6 bHLH TFs and 1 WDR TF were significantly associated with cyanidin-3-O-galactoside. Further, qRT-PCR analysis demonstrated that structural genes (AaPAL3, Aa4CL3, AaCHS2/3/8/9/11, AaDFR1/2, AaANR1, UFGT3a and UFGT6b) and TFs (MYB108, bHLH30, bHLH94-1 and WD43) play important roles in cyanidin biosynthesis. Overall, this study identified cyanidin-3-O-galactoside as the main anthocyanin type and revealed key candidate genes of red coloration of post-harvest fruit in Actinidia arguta. These findings provided new insights into the color formation mechanism of post-harvest fruit and offered a theoretical basis for color regulation in kiwifruit.

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Comparative Metabolome and Transcriptome Analysis of Anthocyanin Biosynthesis in White and Pink Petals of Cotton (Gossypium hirsutum L.)

Cited by 11 publications

References 69 publications

Transcriptome and metabolome analysis reveals the effect of flavonoids on flower color variation in Dendrobium nobile Lindl.

Transcriptome and metabolome analysis reveals the effect of flavonoids on flower color variation in Dendrobium nobile Lindl.

Meloidogyne enterolobiiMeMSP1 effector targets the glutathione‐S‐transferase phi GSTF family in Arabidopsis to manipulate host metabolism and promote nematode parasitism

Transcriptome and metabolome analyses of anthocyanin biosynthesis in post-harvest fruits of a full red-type kiwifruit (Actinidia arguta) ‘Jinhongguan’

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