Comparative metabolomics analysis of <i>Callosobruchus chinensis</i> larvae under hypoxia, hypoxia/hypercapnia and normoxia

Cui, Shishuang; Wang, Lei; Qiu, Jiangping; Liu, Zhicheng; Geng, Xueqing

doi:10.1002/ps.4455

Cited by 39 publications

(20 citation statements)

References 48 publications

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“…Tyrosine (or 4-hydroxyphenylalanine) is an aromatic amino acid that is used by cells to synthesize proteins. Tyrosine is also an antioxidant that can scavenge free radicals and contribute to reduce oxidative damage caused by hypoxic stress [66]. Lysine is an α-amino acid, and an essential amino acid for protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics investigation of molecular responses of whiteleg shrimp (Penaeus vannamei) to hypoxia

Nguyen

Leon²,

Arroyo³

et al. 2020

Preprint

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Hypoxia is a common concern in shrimp aquaculture, affecting growth and survival. Although recent studies have revealed important insights into hypoxia in shrimp and crustaceans, knowledge gaps remain regarding this stressor at the molecular level. In the present study, a gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach was employed to characterize the metabolic pathways underlying responses of shrimp (Penaeus vannamei) to hypoxia and to identify candidate biomarkers. We compared metabolite profiles of shrimp haemolymph before (0 h) and after exposure to hypoxia (1 & 2 h). Dissolved oxygen levels were maintained above 85% saturation in the control and before hypoxia, and 15% saturation in the hypoxic stress treatment. Results showed 44 metabolites in shrimp haemolymph that were significantly different between before and after hypoxia exposure. These metabolites were energy-related metabolites (e.g., TCA cycle intermediates, lactic acids, alanine), fatty acids and amino acids. Pathway analysis revealed 17 pathways that were significantly affected by hypoxia. The changes in metabolites and pathways indicate a shift from aerobic to anaerobic metabolism, disturbance in amino acid metabolism, osmoregulation, oxidative damage and Warburg effect-like response caused by hypoxic stress. Among the altered metabolites, lactic acid was most different between before and after hypoxia exposure. Biomarker analysis also indicated lactic acid as biomarker for hypoxia and model prediction. Future investigations may validate this molecule as a stress biomarker in aquaculture. This study contributes to better understanding of hypoxia in shrimp and crustaceans at the metabolic level and provides a base for future metabolomics investigations on hypoxia.

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Section: Discussionmentioning

confidence: 99%

Metabolomics investigation of molecular responses of whiteleg shrimp (Penaeus vannamei) to hypoxia

Nguyen

Leon²,

Arroyo³

et al. 2020

Preprint

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“…MAs generally involve O 2 , CO 2 , NO, and O 3 . Insect tolerance to hypoxia and hypercapnia critically affects insect control (Cui et al., 2017). O 2 is critical for the survival of aerobic life.…”

Section: Modified Atmosphere Gases Commonly Used For Pest Control Andmentioning

confidence: 99%

“…However, contents of energy substances, including polysaccharides, soluble proteins, and lipids, decreased in a dose- and time-dependent manner in response to CO 2 in larvae of S. paniceum and L. serricorne (Cao et al., 2016a) and adult S. paniceum (Cao et al., 2016b) and O. surinamensis (Cao et al., 2015b). In bean weevil ( Callosobruchus chinensis Linnaeus), Cui et al. (2017) reported that the levels of carbohydrates, amino acids, and organic acids increased, whereas those of free fatty acids decreased in response to hypoxia.…”

Section: Changes In Energy/nutrient Sources Under Modified Atmospherementioning

confidence: 99%

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Role of Modified Atmosphere in Pest Control and Mechanism of Its Effect on Insects

Cao

Zhu

et al. 2019

Front. Physiol.

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Pests not only attack field crops during the growing season, but also damage grains and other food products stored in granaries. Modified or controlled atmospheres (MAs or CAs) with higher or lower concentrations of atmospheric gases, mainly oxygen (O2), carbon dioxide (CO2), ozone (O3), and nitric oxide (NO), provide a cost-effective method to kill target pests and protect stored products. In this review, the most recent discoveries in the field of MAs are discussed, with a focus on pest control as well as current MA technologies. Although MAs have been used for more than 30 years in pest control and play a role in storage pest management, the specific mechanisms by which insects are affected by and adapt to low O2 (hypoxia) and high carbon CO2 (hypercapnia) are not completely understood. Insect tolerance to hypoxia/anoxia and hypercapnia involves a decrease in aerobic metabolism, including decreased NADPH enzyme activity, and subsequently, decreases in glutathione production and catalase, superoxide dismutase, glutathione-S-transferase, and glutathione peroxidase activities, as well as increases in carboxyl esterase and phosphatase activities. In addition, hypoxia induces energy and nutrient production, and in adapted insects, glycolysis and pyruvate carboxylase fluxes are downregulated, accompanied with O2 consumption and acetate production. Consequently, genes encoding various signal transduction pathway components, including epidermal growth factor, insulin, Notch, and Toll/Imd signaling, are downregulated. We review the changes in insect energy and nutrient sources, metabolic enzymes, and molecular pathways in response to modified O2, CO2, NO, and O3 concentrations, as well as the role of MAs in pest control. This knowledge will be useful for applying MAs in combination with temperature control for pest control in stored food products.

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“…Previous studies have paid more attention to this pest control strategies of MA, but little is known about this mechanism of action (Mohapatra et al, 2015). According to our previous research, bean weevil shows high adaptability to hypoxia even hypoxia/hypercapnia (Cui et al, 2017). To further reveal bean weevil hypoxia and hypoxia/hypercapnia tolerance mechanism, we used NextSeq500 RNA-Seq to investigate the differences in gene expression profiles among 4th adzuki bean weevil larvae under hypoxia, hypoxia/hypercapnia and normoxia.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analyses of adzuki bean weevil (Callosobruchus chinensis) response to hypoxia and hypoxia/hypercapnia

Cui

Wang

et al. 2018

Bull. Entomol. Res.

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Stored product insects show high adaption to hypoxia and hypercapnia, but the underlying mechanism is still unclear. Herein, a comparative transcriptome on 4th adzuki bean weevil (Callosobruchus chinensis) instar larvae was studied to clarify the response mechanisms to hypoxia (HA) and hypoxia/hypercapnia (HHA) using NextSeq500 RNA-Seq. Transcript profiling showed a significant difference in HA or HHA exposure both quantitatively and qualitatively. Compared with control, 631 and 253 genes were significantly changed in HHA and HA, respectively. Comparing HHA with HA, 1135 differentially expressed genes (DEGs) were identified. The addition of hypercapnia made a complex alteration on the hypoxia response of bean weevil transcriptome, carbohydrate, energy, lipid and amino acid metabolism were the most highly enriched pathways for genes significantly changed. In addition, some biological processes that were not significantly enriched but important were also discussed, such as immune system and signal transduction. Most of the DEGs related to metabolism both in HHA and HA were up-regulated, while the DEGs related to the immune system, stress response or signal transduction were significantly down-regulated or suppressed. This research reveals a comparatively full-scale result in adzuki bean weevil hypoxia and hypoxia/hypercapnia tolerance mechanism at transcription level, which might provide new insights into the genomic research of this species.

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Comparative metabolomics analysis of Callosobruchus chinensis larvae under hypoxia, hypoxia/hypercapnia and normoxia

Cited by 39 publications

References 48 publications

Metabolomics investigation of molecular responses of whiteleg shrimp (Penaeus vannamei) to hypoxia

Metabolomics investigation of molecular responses of whiteleg shrimp (Penaeus vannamei) to hypoxia

Role of Modified Atmosphere in Pest Control and Mechanism of Its Effect on Insects

Comparative transcriptome analyses of adzuki bean weevil (Callosobruchus chinensis) response to hypoxia and hypoxia/hypercapnia

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