Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota

Yang, Ming; Zhao, Jingjing; Yuan, Yang; Chen, Xiaoyi; Yang, Fan; Li, Xianzhen

doi:10.1007/s10570-020-03671-z

Cited by 12 publications

(6 citation statements)

References 92 publications

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“…The synthesis of cellulases secreted by microorganisms and increased biomass of cellulolytic microbes in the surrounding soil drive continuous decomposition of the biopolymer. 49 Eventually, after being buried for 3 months, the Cel/BT foam completely disappeared, in which cellulose was fully decomposed to nutrients by microorganisms and returned to the carbon cycle, while the inorganic BT went back to the geological cycle, demonstrating an excellent biodegradability of our Cel/BT foam with a lower environmental burden and a closed-loop characteristic toward sustainable applications. Comparatively, the commercial plastic PS foam showed no significant degradation after the same burial time of 3 months, providing evidence of its adverse environmental impact.…”

Section: Resultsmentioning

confidence: 89%

“…After 15 days of burial in the soil, the volume of the Cel/BT foam halved, probably owing to the catabolism of cellulolytic microbes in the natural environment, , whereas the commercially available plastic PS foam maintained its original shape without any change under the same burial condition, suggesting much faster microbial breakdown of the Cel/BT foam in the soil. The synthesis of cellulases secreted by microorganisms and increased biomass of cellulolytic microbes in the surrounding soil drive continuous decomposition of the biopolymer . Eventually, after being buried for 3 months, the Cel/BT foam completely disappeared, in which cellulose was fully decomposed to nutrients by microorganisms and returned to the carbon cycle, while the inorganic BT went back to the geological cycle, demonstrating an excellent biodegradability of our Cel/BT foam with a lower environmental burden and a closed-loop characteristic toward sustainable applications.…”

Section: Resultsmentioning

confidence: 92%

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Scalable Production of Biodegradable, Recyclable, Sustainable Cellulose–Mineral Foams via Coordination Interaction Assisted Ambient Drying

Chen

Wang

et al. 2022

ACS Nano

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Heavy reliance on petrochemical-based plastic foams in both industry and society has led to severe plastic pollution (the so-called “white pollution”). In this work, we develop a biodegradable, recyclable, and sustainable cellulose/bentonite (Cel/BT) foam material directly from resource-abundant natural materials (i.e., lignocellulosic biomass and minerals) via ambient drying. The strong resistance to the capillary force-driven structural collapse of the preformed three-dimensional (3D) network during the ambient drying process can be ascribed to the purpose-designed cellulose–bentonite coordination interaction, which provides a practical way for the locally scalable production of foam materials with designed shapes without complex processing and intensive energy consumption. Benefiting from the strong cellulose–bentonite coordination interaction, the Cel/BT foam material demonstrates high mechanical strength and outstanding thermal stability, outperforming commercial plastic polystyrene foam. Furthermore, the Cel/BT foam presents environmental impacts much lower than those of petrochemical-based plastic foams as it can be 100% recycled in a closed-loop recycling process and easily biodegraded in the environment (natural cellulose goes back to the carbon cycle, and bentonite minerals return to the geological cycle). This study demonstrates an energy-efficient ambient drying approach for the local and scalable production of an all-natural cellulose/bentonite foam for sustainable packaging, buildings, and beyond, presenting great potential in response to “white pollution” and resource shortage.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 92%

Scalable Production of Biodegradable, Recyclable, Sustainable Cellulose–Mineral Foams via Coordination Interaction Assisted Ambient Drying

Chen

Wang

et al. 2022

ACS Nano

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“…Although functional annotations of DEGs have been performed through databases such as GO, KEGG and CAZy, there were still a large number of genes whose functions cannot be annotated. To further explore these unannotated genes potentially related to cellulose metabolism, cluster analysis was performed using the STEM algorithm based on different abundance variation patterns of genes, according the procedures proposed by (Yang et al 2021 ). Based on the abundance variation patterns, functionally unannotated genes were clustered together with annotated cellulolytic genes and were thus assigned a certain function similar to those of annotated cellulolytic genes from the same pattern.…”

Section: Resultsmentioning

confidence: 99%

Differential transcriptome analysis of Sporocytophaga sp. CX11 and identification of candidate genes involved in lignocellulose degradation

Wang

Zhao

Song

et al. 2023

Bioresour. Bioprocess.

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Cellulose is the most abundant renewable bioresources on earth, and the biodegradation and utilization of cellulose would contribute to the sustainable development of global environment. Sporocytophaga species are common aerobic cellulose-degrading bacteria in soil, which can adhere to the surface of cellulose matrix and motile by gliding. In this study, a differential transcriptome analysis of Sporocytophaga sp. CX11 was performed and a total of 4,217 differentially expressed genes (DEGs) were identified. Gene Ontology enrichment results showed that there are three GO categories related to cellulose degradation function among the annotated DEGs. A total of 177 DEGs were identified as genes encoding carbohydrate-active enzymes (CAZymes), among which 54 significantly upregulated CAZymes were mainly cellulases, hemicellulases, pectinases, etc. 39 DEGs were screened to associate with gliding function. In order to explore unannotated genes potentially related to cellulose metabolism, cluster analysis was performed using the Short-Time Series Expression Miner algorithm (STEM). 281 unannotated genes were predicted to be associated with the initial-middle stage of cellulose degradation and 289 unannotated genes might function in the middle-last stage of cellulose degradation. Sporocytophaga sp. CX11 could produce extracellular endo-xylanase, endo-glucanase, FPase and β-glucosidase, respectively, according to different carbon source conditions. Altogether, this study provides valuable insights into the transcriptome information of Sporocytophaga sp. CX11, which would be useful to explore its application in biodegradation and utilization of cellulose resources. Graphical Abstract

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“…When LPY.1was mixed-cultured for 96 h, the enzyme activity reached 2.04 U/mL, which was significantly higher than the enzyme activities recorded for other groups. Cellulose degradation is the result of the synergistic action of cellulases (Yang et al, 2021). LPY.1 group showed the best performance in all enzyme activities and was initially selected as the optimal group of mixed strains (Table 1).…”

Section: Cellulase Production Under Single and Mixed-culture Conditionsmentioning

confidence: 99%

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

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Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota

Cited by 12 publications

References 92 publications

Scalable Production of Biodegradable, Recyclable, Sustainable Cellulose–Mineral Foams via Coordination Interaction Assisted Ambient Drying

Scalable Production of Biodegradable, Recyclable, Sustainable Cellulose–Mineral Foams via Coordination Interaction Assisted Ambient Drying

Differential transcriptome analysis of Sporocytophaga sp. CX11 and identification of candidate genes involved in lignocellulose degradation

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

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