Comparative metagenomic sequencing analysis of cecum microbiotal diversity and function in broilers and layers

Zhao, Qi; Shi, Shuiqin; Tu, Jing; Li, Shaowen

doi:10.1007/s13205-019-1834-1

Cited by 34 publications

(33 citation statements)

References 31 publications

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“…Microbiota composition in poultry, especially in laying hens, has become an important area of research in veterinary medicine [27]. Although some studies have focused on microbiota variation in laying hens at different phases [44][45][46][47] and under diverse production systems [47,48], analyses of microbiota in laying hens fed with plant-derived supplements is limited [49,50]. Given the main role of microbiota in fermentation and digestive processes in laying hens [47], we focused on its composition in the cecum in our analyses.…”

Section: Discussionmentioning

confidence: 99%

Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens

et al. 2019

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The olive oil sector is currently adapting its traditional function to also become a supplier of high-value by-products that possess antioxidant, anti-inflammatory and antimicrobial properties. In this study, we evaluated the effect of the fermented defatted alperujo (FDA) on the intestinal health of laying hens. The morphology of the duodenal and cecal mucosa, the composition of the intestinal microbiota and the productivity of a batch of laying hens were evaluated after FDA supplementation. At early life stages, significant differences (p < 0.001) were observed in duodenal villi height and in crypt depth of both the duodenum and the cecum in the FDA-supplemented group, indicating improved intestinal health in this group. Microbiota composition in the hatchery group supplemented with FDA had a higher abundance of Actinobacteria, Firmicutes and Proteobacteria, and higher bacterial diversity. During the production period, significant differences (p < 0.05) were observed in the number of broken eggs from the supplemented group. We conclude that FDA supplementation improves the absorption capacity of the intestinal mucosa and modifies the intestinal microbiota to favor a greater immune response, leading to an increase in egg production.

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Section: Discussionmentioning

confidence: 99%

Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens

et al. 2019

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“…This is the first work reporting the microbiota and their function along the GIT of healthy donkeys. Previous research on gastrointestinal microbes has mainly focused on cecum [25], and fecal samples [26]of horses. However, there is a great significance to understand the full picture of health microbial communities in the entire GIT of donkey.…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity within the digestive tract contents of Dezhou donkeys

Liu

Bou

et al. 2019

PLoS ONE

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Gastrointestinal microbiota has significant impact on the nutrition and health of monogastric herbivores animals including donkey. However, so far the microbiota in different gastrointestinal compartments of healthy donkey has not been described. Therefore, we investigated the abundance and function of microbiota at different sites of the gastrointestinal tract (GIT) (foregut: stomach, duodenum, jejunum and ileum; hindgut: cecum, ventral colon, dorsal colon, and rectum) of healthy adult donkeys mainly based on 16S rRNA gene sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis. Collectively, our results showed that donkey has a rich, diverse and multi-functional microbiota along the GIT. In general, the richness and diversity of the microbiota are much higher in the hindgut relative to that in the foregut; at phylum level, the Firmicutes is dominant in the foregut while both Firmicutes and Bacteroides are abundant in the hindgut; at the genus level, Lactobacillus was dominant in the foregut while Streptococcus was more dominant in the hindgut. Our further PICRUSt analysis showed that varying microbiota along the GIT is functionally compatible with the corresponding physiological function of different GIT sites. For example, the microbes in the foregut are more active at carbohydrate metabolism, and in the hindgut are more active at amino acid metabolism. This work at the first time characterized the donkey digestive system from the aspects of microbial composition and function, provided an important basic data about donkey healthy gastrointestinal microbiota, which may be utilized to evaluate donkey health and also offer clues to further investigate donkey digestive system, nutrition, even to develop the microbial supplements.

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“…Microbiota of young chickens is highly variable [1][2][3] and the microbiota of adult chickens, i.e., at least 20 weeks of age, has to be used for the definition of core chicken gut microbiota [4][5][6][7]. Proximal parts of the digestive tract of adult chickens are dominated by Lactobacilli, though other species are present as well [8,9].…”

Section: Composition Of Gut Microbiota In Adult Chickensmentioning

confidence: 99%

“…For yet unknown reasons Alistipes spp. belong among the first representatives of phylum Bacteroidetes which colonise the caecum of young chickens [4,6].…”

Section: Major Bacterial Taxa Colonising Chicken Intestinal Tractmentioning

confidence: 99%

“…from family Rikenellaceae is commonly observed as being among the first colonisers from phylum Bacteroidetes, though later during life, bacterial species from families Bacteroidaceae, Prevotellaceae and Porphyromonadaceae represent the most common Gram-negative bacteria in the chicken caecum. The principles of caecal microbiota development have been described independently by many different groups [6,16,[56][57][58][59]. This also means that a comparison of gut microbiota in 4-week-old broilers and 40-week-old layers is biologically confounding and the vast majority of differences in microbiota composition have nothing to do with specifics of broilers and layers, i.e., different genetics, but mainly with different age.…”

Section: Development Of Gut Microbiota In Commercially Hatched Chickensmentioning

confidence: 99%

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Composition and Function of Chicken Gut Microbiota

Rychlı́k

2020

Animals

265

203

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Studies analyzing the composition of gut microbiota are quite common at present, mainly due to the rapid development of DNA sequencing technologies within the last decade. This is valid also for chickens and their gut microbiota. However, chickens represent a specific model for host–microbiota interactions since contact between parents and offspring has been completely interrupted in domesticated chickens. Nearly all studies describe microbiota of chicks from hatcheries and these chickens are considered as references and controls. In reality, such chickens represent an extreme experimental group since control chicks should be, by nature, hatched in nests in contact with the parent hen. Not properly realising this fact and utilising only 16S rRNA sequencing results means that many conclusions are of questionable biological relevance. The specifics of chicken-related gut microbiota are therefore stressed in this review together with current knowledge of the biological role of selected microbiota members. These microbiota members are then evaluated for their intended use as a form of next-generation probiotics.

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Comparative metagenomic sequencing analysis of cecum microbiotal diversity and function in broilers and layers

Cited by 34 publications

References 31 publications

Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens

Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens

Microbial diversity within the digestive tract contents of Dezhou donkeys

Composition and Function of Chicken Gut Microbiota

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