Comparative microrheology of blood: effect of desaggregation and cell fluidity on shear thinning of human and bovine blood123

Schmid‐Schönbein, H.; Gosen, J. v.; Klose, Holger

doi:10.3233/bir-1973-10407

Cited by 4 publications

(1 citation statement)

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“…Bovine RBCs do not form any aggregate under physiologic conditions [17]. The modification of the cell surface by trypsin induced rouleaux formation, whereas the modification of the cell surface by neuraminidase did not cause any aggregate formation [18].…”

Section: Introductionmentioning

confidence: 98%

Effects of trypsin on aggregation, disaggregation and aggregate morphology of red blood cells in autologous plasma and serum

2021

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We study the influence of trypsin on aggregation, disaggregation, and aggregate morphology of RBCs in autologous plasma and serum. The effect of trypsin on the surface charge of red blood cells and the aggregation of glutaraldehyde fixed cells after treatment with the enzyme was also studied. RBC aggregation was studied by means of an aggregometer and microscopic observations. The results obtained in this study indicate that trypsin treatment increases RBCs aggregation in autologous plasma and serum. The disaggregation of erythrocytes after trypsin treatment considerably decreased in autologous plasma and serum. Increase in the strength of red blood cell aggregates was observed in autologous plasma and serum. The microscopic images of RBCs aggregates indicate the formation of globular (pathologic) structures of aggregates in autologous plasma and serum. Trypsin decrease the surface charge of RBCs. In autologous plasma and serum, the cup shapes of RBCs appear. The control RBCs fixed with glutaraldehyde were not aggregated after their placement in autologous plasma. At the same time, red blood cells pretreated with trypsin and fixed with glutaraldehyde interact with each other in autologous plasma. The physiological significance of glycoproteins of erythrocyte surface for RBCs aggregation was discussed.

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Section: Introductionmentioning

confidence: 98%