Comparative mitogenic response of old and young Rhesus monkey T cells to lectin from Erythrina cristagalli

The influence of aging and dietary restriction on increase in intracellular free calcium ([Ca2+]i) of CD4+ lymphocytes from Macaca mulatta was examined after stimulation with anti-CD3 mAb. We used a flow cytometric assay with the dye indo-1 and either direct or reciprocal immunofluorescent staining to identify CD4+ cells. After stimulation with anti-CD3 mAb, intracellular free calcium responses were reduced in CD4+ lymphocytes from old male and female ad libitum fed monkeys compared to young and adult male or female monkeys. Old female monkeys had significantly lower [Ca2+]i than did old male monkeys. The reduced responses were in part related to a decreased percentage of responding cells. Dietary restriction of males over a four-year period did not alter [Ca2+]i response compared to ad libitum fed male monkeys. Female monkeys of all ages (which were restricted only for four months) also had similar [Ca2+]i responses to ad libitum fed controls. Our data suggest that age-related changes in [Ca2+]i responses are similar between humans and M. mulatta, and that over these intervals, no effects of caloric restrictions can be detected.

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Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4⁺ lymphocytes in rhesus monkeys (Macaca mulatta)

Grossmann

Rabinovitch

Lane

et al. 1995

Journal Cellular Physiology

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Inhibition of T cell mitogenesis by nitrofurans

Mercado

Molina

Navas

et al. 1991

Biochemical Pharmacology

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Comparative mitogenic response of old and young Rhesus monkey T cells to lectin from Erythrina cristagalli

Cited by 2 publications

References 18 publications

Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4⁺ lymphocytes in rhesus monkeys (Macaca mulatta)

Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4⁺ lymphocytes in rhesus monkeys (Macaca mulatta)

Inhibition of T cell mitogenesis by nitrofurans

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Comparative mitogenic response of old and young Rhesus monkey T cells to lectin from Erythrina cristagalli

Cited by 2 publications

References 18 publications

Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4+ lymphocytes in rhesus monkeys (Macaca mulatta)

Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4+ lymphocytes in rhesus monkeys (Macaca mulatta)

Inhibition of T cell mitogenesis by nitrofurans

Contact Info

Product

Resources

About

Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4⁺ lymphocytes in rhesus monkeys (Macaca mulatta)

Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4⁺ lymphocytes in rhesus monkeys (Macaca mulatta)