Comparative Molecular-Genetic Analysis of Mobile Elements in Natural Strains of Cholera Agent

Smirnova, N. I.; Zadnova, S. P.; Agafonov, D. A.; Shashkova, Anna; Cheldyshova, N. B.; Cherkasov, Alexander

doi:10.7868/s0016675813090087

Cited by 12 publications

(3 citation statements)

References 21 publications

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“…The principal virulence determinants are cholera toxin and toxin-coregulated pili ( 1 ). Recent studies of the genome of V. cholerae strains from previous and current epidemics have demonstrated that genomic changes and alteration in the cholera toxin prophage (CTX) of typical El Tor strains resulted in the emergence of new variants ( 2 – 4 ). Here, we report the first draft whole-genome sequences of two V. cholerae O1 El Tor strains.…”

Section: Genome Announcementmentioning

confidence: 99%

Whole-Genome Sequencing of Vibrio cholerae O1 El Tor Strains Isolated in Ukraine (2011) and Russia (2014)

et al. 2017

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Section: Genome Announcementmentioning

confidence: 99%

Whole-Genome Sequencing of Vibrio cholerae O1 El Tor Strains Isolated in Ukraine (2011) and Russia (2014)

et al. 2017

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“…хт кодируется генами оперона ctxAB, расположенного на профаге стхφ. за синтез ткпа отвечают гены кластера tcpAF, расположенные на острове патогенности VPI-1 (от Vibrio pathogenicity island) [1,2]. на том же острове располагаются и регуляторные гены (tcpP, tcpH и toxT), которые совместно с регуляторным геном toxR, а также генами оперона ctxAB и кластера генов tcpAF входят в генную сеть вирулентности холерного вибриона [3,4].…”

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“…высказывалось даже предположение, что появление штаммов O139серогруппы станет началом восьмой пандемии холеры [11]. однако эти опасения не оправдались, и на сегодняшний день геноварианты практически полностью вытеснили других возбудителей холеры [2,12,13]. геноварианты V. cholerae биовара эль тор обладают рядом генетических особенностей, отличающих их от типичных штаммов.…”

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Comparative Analysis of Expression of the Main Virulence Genes in Various <i>Vibrio cholerae</i> О1 Strains

Cheldyshova

Devdariani

2022

Problemy Osobo Opasnykh Infektsii

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The aim of the work was a comparative study of the expression of the main virulence genes in Vibrio cholerae strains of the classical biovar, typical and genetically modified strains of V. cholerae, El Tor biovar.Materials and methods. Natural toxigenic strains of V. cholerae O1, classical biovar (J89, Pakistan, 1969), typical (M-887, Astrakhan, 1970) and genetically modified (301, Taganrog, 2011) strains of the El Tor biovar were used as model ones. The strains were grown under optimum conditions for the production of cholera toxin and toxin-coregulated pili. The assessment of strain growth was carried out in LB broth at room temperature with determination of the cells number on a Biowave DNA spectrophotometer (Biochrome Ltd., UK). Determination of gene expression was performed using real-time PCR with reverse transcription.Results and discussion. The expression of structural (ctxA, tcpA) and regulatory (toxR, toxT, tcpP, tcpH) virulence genes has been investigated in V. cholerae strains of the classical biovar, typical and genetically modified strains of the El Tor biovar. Significant differences have been revealed in terms of time and level of maximum expression of these genes in strains of classical and El Tor biovars. It was found that ctxA and toxR genes expression in the genovariant strain reached its maximum 1–3 h earlier than in the other strains. At the same time, the level of ctxA gene expression corresponded to the level of the classical strain. The maximum expression of the toxR gene in the genovariant strain was higher than in typical El Tor and classical strains, and also had a clear inverse correlation with ctxA gene expression. Expression of the tcpA, toxT, and tcpH genes in the classical biovar strain reached its maximum 1–2 h earlier than in the El Tor biovar strains. These differences should be taken into account when conducting research work related to the study of the expression of the main virulence genes.

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Structural and functional changes of the genome in avirulent Vibrio cholerae strains biovar El Tor, genotype ctxA+tcpA+

Smirnova¹,

Agafonov²,

Shchelkanova³

et al. 2020

Mol. genet. mikrobiol. virusol.

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Comparative Molecular-Genetic Analysis of Mobile Elements in Natural Strains of Cholera Agent

Cited by 12 publications

References 21 publications

Whole-Genome Sequencing of Vibrio cholerae O1 El Tor Strains Isolated in Ukraine (2011) and Russia (2014)

Whole-Genome Sequencing of Vibrio cholerae O1 El Tor Strains Isolated in Ukraine (2011) and Russia (2014)

Comparative Analysis of Expression of the Main Virulence Genes in Various <i>Vibrio cholerae</i> О1 Strains

Structural and functional changes of the genome in avirulent Vibrio cholerae strains biovar El Tor, genotype ctxA+tcpA+

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