Blueberry (Vaccinium corymbosum)
cultivars vary in firmness, and these phenotypic differences may be
associated with peel and pulp cell wall polysaccharides. Three blueberry
cultivars of distinctive texture phenotypes, Indigocrisp (crisp),
Emerald (firm, industry standard), and Jewel (soft), were evaluated
for cell wall polysaccharide composition. Alcohol-insoluble residues
(AIRs) from both peel and pulp were reduced, methylated, hydrolyzed,
acetylated, and quantified using gas chromatography–mass spectrometry
(GC-MS). Monosaccharide composition (μg·mg–1 AIR) differed among cultivars, with “Indigocrisp”
pulp highest in glucuronic acid (22.23), “Emerald” pulp
highest in glucose (106.31), and “Jewel” peel highest
in arabinose (38.73) and mannose (11.88). Forty-five cell wall polysaccharide
linkages were identified, and specific linkages were associated with
blueberry peel and pulp among the texture phenotypes. Polysaccharide
classifications were then estimated from the 45 cell wall polysaccharide
linkages. “Indigocrisp” and “Emerald”
pulp were highest in arabinan and type II arabinogalactan, which are
less susceptible to depolymerization. “Indigocrisp”
pulp had a greater abundance of heteromannan, xyloglucan, and cellulose,
while “Jewel” was highest in rhamnogalacturonan I, which
typically depolymerizes first. The greater abundances of arabinan
and type II arabinogalactan in the pulp of the firm and crisp cultivars
likely contribute to the texture characteristics of these phenotypes.