Comparative mutagenicity of hydrazine and 3 methylated derivatives in L5178Y mouse lymphoma cells

Opinion of the Scientific Panel on Plant protection products and their residues (PPR) related to the evaluation of daminozide in the context of Council Directive 91/414/EEC

“…In the studies on gene mutation in mouse lymphoma L5178Y cells at the thymidine kinase locus (TK +/-), positive results in the absence and the presence of metabolic activation systems (S9 mix) were described by Brusick and Matheson (1976). In the study by Rogers and Back (1981) mutation was observed at the TK locus, but not at the ouabain resistance or cytosine arabinoside resistance loci.…”

Effects of Pollutants on Vertebrate Cells in Vitro.

“…Electrodes radiography was performed in total darkness as fol-were selected with resistances between 20 and 50 lows: the slides were dipped into a 3317 aqueous megohms. Individual contracting myocardial cells solution of Ilford nuclear research emulsion type [1][2][3][4] were selected for impalement by observation through (batch PL-729. Ciba-Geigy Co.. Ilford.…”

“…Because of the widespread use of hydrazines as a major fuel propellant and as an oxygen scavenger in industry, the biological effects of these compounds is of significant concern. Studies have dealt with the mutagenecity of the hydrazines (Kimball, 1977, Rogers andBack, 1981), their carcinogenetic potenteial ), and their metabolic toxicity (Dost et al, 1979). In addition, hydrazines have been shown to have teratogenic effects on mammalian (Schiller et al, 1979) and amphibian embryonic development (Greenhouse, 1976).…”

Dimethylhydrazine

“…In a study using cultured L5178Y mouse lymphoma cells, Rogers and Back (1981) reported that both 1,1-dimethylhydrazine and 1,2-dimethylhydrazine induced forward mutations at the thymidine kinase level in the absence of an extraneous metabolic activation system. The investigators also noted that the two dimethylhydrazines appeared to have different modes of action under these conditions.…”