Comparative Organogenic Response of Six Clonal Apple Rootstock Cultivars

Sun, Qiangming; Sun, Miao; Sun, Hongyan; Bell, Richard L.; Li, Linguang; Wei, Zhang; Tao, Jihan

doi:10.21273/hortsci.51.3.272

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…Micropropagated rootstock trees (M26, G935, and MM106) were grown axenically with methods similar to Dobranszki and da Silva ( 2010 ); Sun et al ( 2016 ) and Yepes and Adwinckle ( 1994 ). Shoots were grown in sterile Magenta boxes (GA-7, Sigma Aldrich) in a growth chamber (12 h light at 25°C/ 12 h dark 20°C) in shoot multiplication media (SMM) to the height of ~4 cm, and then sterilely excised from the callus and transferred to rooting induction media (RIM).…”

Section: Methodsmentioning

confidence: 99%

Targeted Metabolic Profiling Indicates Apple Rootstock Genotype-Specific Differences in Primary and Secondary Metabolite Production and Validate Quantitative Contribution From Vegetative Growth

2018

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Previous reports regarding rhizodeposits from apple roots are limited, and complicated by microbes, which readily colonize root systems and contribute to modify rhizodeposit metabolite composition. This study delineates methods for collection of apple rhizodeposits under axenic conditions, indicates rootstock genotype-specific differences and validates the contributions of vegetative activity to rhizodeposit quantity. Primary and phenolic rhizodeposit metabolites collected from two apple rootstock genotypes, G935 and M26, were delineated 2 months after root initiation by utilizing gas chromatography/liquid chromatography—mass spectrometry (GC/LC-MS), respectively. Twenty-one identified phenolic compounds and 29 sugars, organic acids, and amino acids, as well as compounds tentatively identified as triterpenoids were present in the rhizodeposits. When adjusted for whole plant mass, hexose, erythrose, galactose, phloridzin, kaempferol-3-glucoside, as well as glycerol, and glyceric acid differed between the genotypes. Phloridzin, phloretin, epicatechin, 4-hydroxybenzoic acid, and chlorogenic acid were among the phenolic compounds found in higher relative concentration in rhizodeposits, as assessed by LC-MS. Among primary metabolites assessed by GC-MS, amino acids, organic acids, and sugar alcohols found in relatively higher concentration in the rhizodeposits included L-asparagine, L-cysteine, malic acid, succinic acid, and sorbitol. In addition, putative ursane triterprenoids, identified based on accurate mass comparison to previously reported triterpenoids from apple peel, were present in rhizodeposits in high abundance relative to phenolic compounds assessed via the same extraction/instrumental method. Validation of metabolite production to tree vegetative activity was conducted using a separate set of micropropagated trees (genotype MM106) which were treated with a toxic volatile compound (butyrolactone) to inhibit activity/kill leaves and vegetative growth. This treatment resulted in a reduction of total collected rhizodeposits relative to an untreated control, indicating active vegetative growth contributes to rhizodeposit metabolites. Culture-based assays indicated an absence of bacterial or fungal endophytes in roots of micropropagated G935 and M26 plants. However, the use of fungi-specific primers in qPCR indicated the presence of fungal DNA in 30% of the samples, thus the contribution of endophytes to rhizodeposits cannot be fully eliminated. This study provides fundamental information for continued research and application of rhizosphere ecology driven by apple rootstock genotype specific rhizodeposition.

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Section: Methodsmentioning

confidence: 99%

Targeted Metabolic Profiling Indicates Apple Rootstock Genotype-Specific Differences in Primary and Secondary Metabolite Production and Validate Quantitative Contribution From Vegetative Growth

2018

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“…The efficiency of IBA in inducing roots was well documented in other systems such as Tylophora indica [43], Oldenlandia corymbose [44], and Gymnostachyum febrifugum [45]. In other plant species, such as gallnut trees [46], Chinese tallow trees [47], cherry trees [48] and apple trees [49], a low level of macronutrients in the medium was more favorable for in vitro rooting. The reason may be that the concentration of macronutrients required for rooting is much lower than that required for shoot induction and growth.…”

Section: (A) (C) (B) (D) (F) (E)mentioning

confidence: 98%

Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil

Klaocheed,

Rittirat,

Kalawong

et al. 2024

Trends Sci

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Petiole segments of an economically ornamental plant Oxalis triangularis A.st.-Hil have been used in the present study for rapid, reliable and systematic in vitro propagation. The morphogenic effect of α-Naphthalene acetic acid (NAA) at various concentrations (0.5 - 3.0 mg/L) was studied individually or in combination with 6-benzylaminopurine (BAP). Superior multiplication rates were achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP. Of the tested combinations, maximum shoot regeneration (100.00 %), mean shoot number (14.60 ± 0.37 shoots per segment), and shoot length (18.11 ± 0.30 mm) were recorded on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP after 6 weeks of incubation. Half-strength MS medium augmented with 1.0 mg/L indole-3-butyric acid (IBA) was the optimal medium for in vitro root induction, (24.60 ± 0.37 roots per shoot with 9.10 ± 0.32 mm in length) after 6 weeks of incubation. The regenerated plantlets showed 100 % survival success during acclimatization and exhibited normal growth characteristics and morphology. This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil. HIGHLIGHTS The tissue culture technology was successfully employed for developing a practicable simple, efficient, and amenable protocol for mass multiplication of Oxalis triangularis A.st.-Hil, an economically ornamental plant Our studies based on interaction and combinations of auxins with BAP provided better shoot proliferation The regenerated plantlets survived well under field conditions This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil GRAPHICAL ABSTRACT

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Efficient plant regeneration mediated by root segment-derived juvenile shoots of Rhus chinensis Mill

Hou

Ding

et al. 2023

Trees

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Comparative Organogenic Response of Six Clonal Apple Rootstock Cultivars

Cited by 8 publications

References 31 publications

Targeted Metabolic Profiling Indicates Apple Rootstock Genotype-Specific Differences in Primary and Secondary Metabolite Production and Validate Quantitative Contribution From Vegetative Growth

Targeted Metabolic Profiling Indicates Apple Rootstock Genotype-Specific Differences in Primary and Secondary Metabolite Production and Validate Quantitative Contribution From Vegetative Growth

Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil

Efficient plant regeneration mediated by root segment-derived juvenile shoots of Rhus chinensis Mill

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