Comparative Osteogenesis of Radiopaque Dicalcium Silicate Cement and White-Colored Mineral Trioxide Aggregate in a Rabbit Femur Model

Wu, Buor-Chang; Huang, Soon-Cen; Ding, Shinn-Jyh

doi:10.3390/ma6125675

Cited by 20 publications

(10 citation statements)

References 41 publications

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“…Cell viability and functions associated with implants are closely related to the physical, chemical, and biological characteristics of the materials used [ 9 , 25 ], which in turn affect in vivo efficacy [ 26 ]. To elucidate the effects of plasma treatment and surface roughness on osteogenic activities, the biological functions of MG63 cells cultured on samples were evaluated.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Hydrophilicity and Biocompatibility of Dental Zirconia Ceramics by Oxygen Plasma Treatment

Wei

et al. 2015

Materials

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Surface properties play a critical role in influencing cell responses to a biomaterial. The objectives of this study were (1) to characterize changes in surface properties of zirconia (ZrO2) ceramic after oxygen plasma treatment; and (2) to determine the effect of such changes on biological responses of human osteoblast-like cells (MG63). The results indicated that the surface morphology was not changed by oxygen plasma treatment. In contrast, oxygen plasma treatment to ZrO2 not only resulted in an increase in hydrophilicity, but also it retained surface hydrophilicity after 5-min treatment time. More importantly, surface properties of ZrO2 modified by oxygen plasma treatment were beneficial for cell growth, whereas the surface roughness of the materials did not have a significant efficacy. It is concluded that oxygen plasma treatment was certified to be effective in modifying the surface state of ZrO2 and has the potential in the creation and maintenance of hydrophilic surfaces and the enhancement of cell proliferation and differentiation.

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Section: Resultsmentioning

confidence: 99%

Enhanced Hydrophilicity and Biocompatibility of Dental Zirconia Ceramics by Oxygen Plasma Treatment

Wei

et al. 2015

Materials

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“…The release of biologically active ions and the formation of a calcium phosphate layer on the surface may affect the behavior and gene expression of hAD-MSCs and trigger their osteogenic commitment. Indeed, calcium and silicon ions released from CaSi demonstrated to stimulate mineralizing cells proliferation [43][44][45]47,[56][57][58][59][60] enhancing the expression of several genes, such as pro-osteogenic genes (as osteocalcin, bone sialoprotein and alkaline phosphatase) [42][43][44][45][77][78][79] and pro-angiogenic genes [60]. PLA-based CaSi-DCPD-doped scaffolds revealed the ability to support the growth of vascular wall mesenchymal stem cells, stimulating the gene expression of several pro-angiogenic markers, including platelet-derived growth factor receptor-β (PDGFR-β), alpha smooth muscle actin (α-SMA), neuron-glial antigen 2 (NG2), and CD 90 [60].…”

Section: Discussionmentioning

confidence: 99%

Mineral-Doped Poly(L-lactide) Acid Scaffolds Enriched with Exosomes Improve Osteogenic Commitment of Human Adipose-Derived Mesenchymal Stem Cells

et al. 2020

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Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced phase separation technique, as materials for bone regeneration. The aim of this study was to investigate the effect of mineral-doped PLA-based porous scaffolds enriched with exosome vesicles (EVs) on osteogenic commitment of human adipose mesenchymal stem cells (hAD-MSCs). Two different mineral-doped scaffolds were produced: PLA-10CaSi-10DCPD and PLA-5CaSi-5DCPD. Scaffolds surface micromorphology was investigated by ESEM-EDX before and after 28 days immersion in simulated body fluid (HBSS). Exosomes were deposited on the surface of the scaffolds and the effect of exosome-enriched scaffolds on osteogenic commitment of hAD-MSCs cultured in proximity of the scaffolds has been evaluated by real time PCR. In addition, the biocompatibility was evaluated by direct-contact seeding hAD-MSCs on scaffolds surface-using MTT viability test. In both formulations, ESEM showed pores similar in shape (circular and elliptic) and size (from 10–30 µm diameter). The porosity of the scaffolds decreased after 28 days immersion in simulated body fluid. Mineral-doped scaffolds showed a dynamic surface and created a suitable bone-forming microenvironment. The presence of the mineral fillers increased the osteogenic commitment of hAD-MSCs. Exosomes were easily entrapped on the surface of the scaffolds and their presence improved gene expression of major markers of osteogenesis such as collagen type I, osteopontin, osteonectin, osteocalcin. The experimental scaffolds enriched with exosomes, in particular PLA-10CaSi-10DCPD, increased the osteogenic commitment of MSCs. In conclusion, the enrichment of bioresorbable functional scaffolds with exosomes is confirmed as a potential strategy to improve bone regeneration procedures.

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“…Antibacterial dicalcium silicate cement Wu et al Ding 2010, Chiang et al 2014). However, it has the advantages of shorter setting time (<30 min) (Chiang & Ding 2010) and colour stability (Wu et al 2013). Nevertheless, further investigation is needed for preclinical trials.…”

Section: Figurementioning

confidence: 99%

Comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white‐coloured mineral trioxide aggregate

Wei

Hsueh

et al. 2014

Int Endodontic J

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Comparative Osteogenesis of Radiopaque Dicalcium Silicate Cement and White-Colored Mineral Trioxide Aggregate in a Rabbit Femur Model

Cited by 20 publications

References 41 publications

Enhanced Hydrophilicity and Biocompatibility of Dental Zirconia Ceramics by Oxygen Plasma Treatment

Enhanced Hydrophilicity and Biocompatibility of Dental Zirconia Ceramics by Oxygen Plasma Treatment

Mineral-Doped Poly(L-lactide) Acid Scaffolds Enriched with Exosomes Improve Osteogenic Commitment of Human Adipose-Derived Mesenchymal Stem Cells

Comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white‐coloured mineral trioxide aggregate

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