Comparative PCR analyses for the detection of the Cape St. Paul wilt disease phytoplasma in coconut palms in Ghana

Obeng‐Darko, Sylvester A.; Quaicoe, Robert N.; Yankey, Egya Ndede; Twumasi, Peter

doi:10.1111/jph.12707

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…To date, molecular characterization of “ Ca . Phytoplasma palmicola” has been based mainly on the amplification of the 16S-23S rRNA gene operon by using the universal phytoplasma 16S rRNA gene primers, P1/P7 (47, 48), or specific primers (49), or, alternatively, on the amplification of the ribosomal protein gene (50) or the secA gene (51, 52). The MLST scheme we report is based on eight housekeeping genes and allows the deciphering of “ Ca .…”

Section: Discussionmentioning

confidence: 99%

Multilocus Sequence Analysis Reveals Three Distinct Populations of “ Candidatus Phytoplasma palmicola” with a Specific Geographical Distribution on the African Continent

Pilet

Quaicoe

Osagie

et al. 2019

Appl Environ Microbiol

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To sustain epidemiological studies on coconut lethal yellowing disease (CLYD), a devastating disease in Africa caused by a phytoplasma, we developed a multilocus sequence typing (MLST) scheme for “CandidatusPhytoplasma palmicola” based on eight housekeeping genes. At the continental level, eight different sequence types were identified among 132 “CandidatusPhytoplasma palmicola”-infected coconuts collected in Ghana, Nigeria, and Mozambique, where CLYD epidemics are still very active. “CandidatusPhytoplasma palmicola” appeared to be a bacterium that is subject to strong bottlenecks, reducing the fixation of positively selected beneficial mutations into the bacterial population. This phenomenon, as well as a limited plant host range, might explain the observed country-specific distribution of the eight haplotypes. As an alternative means to increase fitness, bacteria can also undergo genetic exchange; however, no evidence for such recombination events was found for “CandidatusPhytoplasma palmicola.” The implications for CLYD epidemiology and prophylactic control are discussed. The usefulness of seven housekeeping genes to investigate the genetic diversity in the genus “CandidatusPhytoplasma” is underlined.IMPORTANCECoconut is an important crop for both industry and small stakeholders in many intertropical countries. Phytoplasma-associated lethal yellowing-like diseases have become one of the major pests that limit coconut cultivation as they have emerged in different parts of the world. We developed a multilocus sequence typing scheme (MLST) for tracking epidemics of “Ca. Phytoplasma palmicola,” which is responsible for coconut lethal yellowing disease (CLYD) on the African continent. MLST analysis applied to diseased coconut samples collected in western and eastern African countries also showed the existence of three distinct populations of “Ca. Phytoplasma palmicola” with low intrapopulation diversity. The reasons for the observed strong geographic patterns remain to be established but could result from the lethality of CLYD and the dominance of short-distance insect-mediated transmission.

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Section: Discussionmentioning

confidence: 99%

Multilocus Sequence Analysis Reveals Three Distinct Populations of “ Candidatus Phytoplasma palmicola” with a Specific Geographical Distribution on the African Continent

Pilet

Quaicoe

Osagie

et al. 2019

Appl Environ Microbiol

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Integrated soil fertility management using cocoa bean shells improves soil chemical properties, coconut yield and mitigates environmental pollution

Andoh-Mensah,

Anthonio,

Sossah

et al. 2023

Journal of Cleaner Production

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Towards Pathogen-Free Coconut Germplasm Exchange

Yang,

Nguyen,

Nulu

et al. 2024

Plants

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Coconut (Cocos nucifera L.) is an important palm species that serves as the mainstay of several industries and contributes to the livelihoods of millions of smallholder farmers. International exchange of coconut germplasm has been undertaken for several decades to facilitate the conservation of selected varieties within global genebanks and for the distribution to farmers and scientists. In vitro systems are a convenient and an efficient method for the exchange of coconut germplasm. However, it is possible that these tissue culture systems can transfer lethal pathogens causing a threat to the importing countries. In this review, the following topics are discussed: the major disease-causing agents of concern, the various tissues that could be used for coconut germplasm exchange, and the techniques available for the detection and elimination of disease-causing agents from various transmission systems. Additionally, the lack of clear, science-backed guidelines to facilitate the exchange of in vitro coconut materials is raised, along with recommendations for future studies to ensure the safe movement of coconut germplasm without biosecurity risks.

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Comparative PCR analyses for the detection of the Cape St. Paul wilt disease phytoplasma in coconut palms in Ghana

Cited by 3 publications

References 20 publications

Multilocus Sequence Analysis Reveals Three Distinct Populations of “ Candidatus Phytoplasma palmicola” with a Specific Geographical Distribution on the African Continent

Multilocus Sequence Analysis Reveals Three Distinct Populations of “ Candidatus Phytoplasma palmicola” with a Specific Geographical Distribution on the African Continent

Integrated soil fertility management using cocoa bean shells improves soil chemical properties, coconut yield and mitigates environmental pollution

Towards Pathogen-Free Coconut Germplasm Exchange

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