Comparative Performance of a New SARS-CoV-2 Rapid Detection System

Savellini, Gianni Gori; Anichini, Gabriele; Terrosi, Chiara; Prathyumnan, Shibily; Marini, Stefano; Cusi, Maria Grazia

doi:10.1128/spectrum.00205-21

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…Unlike RVFV filamentous NSs in the nucleus, SFTSV, Toscana virus (TOSV) and Uukuniemi virus (UUKV) NSs proteins localize only in the cytoplasm, so they could not directly inhibit host transcription. Besides the unique cytoplasmic granules generated by SFTSV NSs to sequester numerous host factors, TOSV NSs degrades PKR to facilitate viral translation similarly with RVFV, but TOSV NSs could also degrade RIG-I to suppress IFN-b signal activation with its E3 ubiquitin ligase activity (86,87). NSs of the Punta Toro virus (PTV) can inhibit host transcription, but the nuclear NSs does not form a filamentous structure (88).…”

Section: Discussionmentioning

confidence: 99%

Arm race between Rift Valley fever virus and host

et al. 2022

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Rift Valley fever (RVF) is a zoonotic disease caused by Rift Valley fever virus (RVFV), an emerging arbovirus within the Phenuiviridae family of Bunyavirales that has potential to cause severe diseases in both humans and livestock. It increases the incidence of abortion or foetal malformation in ruminants and leads to clinical manifestations like encephalitis or haemorrhagic fever in humans. Upon virus invasion, the innate immune system from the cell or the organism is activated to produce interferon (IFN) and prevent virus proliferation. Meanwhile, RVFV initiates countermeasures to limit antiviral responses at transcriptional and protein levels. RVFV nonstructural proteins (NSs) are the key virulent factors that not only perform immune evasion but also impact the cell replication cycle and has cytopathic effects. In this review, we summarize the innate immunity host cells employ depending on IFN signal transduction pathways, as well as the immune evasion mechanisms developed by RVFV primarily with the inhibitory activity of NSs protein. Clarifying the arms race between host innate immunity and RVFV immune evasion provides new avenues for drug target screening and offers possible solutions to current and future epidemics.

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Section: Discussionmentioning

confidence: 99%

Arm race between Rift Valley fever virus and host

et al. 2022

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“…[12][13][14] Rapid antigen test, Rapid antibody test, RT-PCR, Loop-mediated isothermal amplification, CRISPR, and Next-generation sequencing are SARS-CoV-2 diagnostic assays. 15,16 The diagnosis of both SARS-CoV-2 and influenza infections is still reliant on RT-PCR as the gold standard. 17 In the present study, we aimed to describe the clinical manifestation and laboratory biomarkers of patients hospitalized with COVID-19 and Flu infections.…”

Section: Introductionmentioning

confidence: 99%

“…To date, several molecular and serological assays have been introduced to the detection of SARS‐CoV‐2 and Flu viruses 12–14 . Rapid antigen test, Rapid antibody test, RT‐PCR, Loop‐mediated isothermal amplification, CRISPR, and Next‐generation sequencing are SARS‐CoV‐2 diagnostic assays 15,16 . The diagnosis of both SARS‐CoV‐2 and influenza infections is still reliant on RT‐PCR as the gold standard 17 …”

Section: Introductionmentioning

confidence: 99%

Evaluating the efficiency of ELISA, monoplex and multiplex probe‐based real‐time reverse‐transcription PCR assays in the detection of SARS‐CoV‐2 (COVID‐19) and influenza A and B viruses: A cross‐sectional study

Mosadegh,

Jalili,

Pourmand

et al. 2024

Health Science Reports

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Background and AimsThe current study aimed to evaluate the efficiency of Enzyme‐linked immunosorbent assay (ELISA) assay and monoplex and multiplex real‐time reverse‐transcription PCR (rRT‐PCR) in the detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and influenza A and B viruses (Flu A and Flu B).MethodsThe SARS‐CoV‐2 ‐specific IgG and IgM antibodies, as well as, Flu A (H1N1 and H3N2 serotypes) and Flu B virus antibodies were determined by ELISA assay. The one‐step qRT‐PCR method was used to detect the SARS‐CoV‐2 in nasopharyngeal swab samples. Furthermore, the presence of Flu A and B viruses was evaluated using probe‐based RT‐PCR. Simultaneous detection of SARS‐CoV‐2, Flu A and B viruses was performed by multiplex rRT‐PCR assay.ResultsSARS CoV‐2 IgM and IgG antibodies were detected in 33.3% and 58.3% of patients, respectively. In contrast, the SARS CoV‐2 genome was detected in 50% of patients using the one‐step monoplex RT‐PCR assay. Flu A serotypes H1N1 and H3N2 were found in 16.7% and 8.3% of patients. Probe‐based RT‐PCR revealed that 39.3% of patients were positive for the Flu A virus. Multiplex rRT‐PCR detect the SARS‐CoV‐2, Flu A, and Flu B in 50%, 39.3%, and 19% of samples, respectively. The sensitivity and specificity of multiplex rRT‐PCR assay in comparison to monoplex RT‐PCR were 100% and 55%, respectively. Coinfection with SARS‐CoV‐2, Flu A, and Flu B viruses was found in 9.5% of patients.ConclusionMultiplex rRT‐PCR can be used as a repaid, cost‐effective and suitable tool for molecular surveillance of SARS‐CoV‐2 and Flu A/B viruses.

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Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way

Padoan

Cosma

Aita

et al. 2022

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives The reliable identification of individuals with SARS-CoV-2 infection is the cornerstone for containing viral spread. Rapid molecular point-of-care testing (POCT) of saliva might reduce analysis time, thus increasing the efficacy of contact tracing. In this study, a new POCT RT-PCR assay for the detection of SARS-CoV-2 RNA in saliva was evaluated and compared with an already validated CE-IVD method. Methods An evaluation was made of 160 left-over salivary samples (27 frozen, kept at −80 °C and 133 fresh), collected using Salivette (Sarstedt, Germany). Samples were analyzed by TaqPath COVID-19 CE-IVD RT-PCR kit, QuantStudio5 Real-Time (Applied Biosystems, USA) (TaqPath) and bKIT Virus Finder COVID-19 Saliva (Hyris Global Diagnostics, Italy). Performances of three- and fivefold pooling strategies were also evaluated. Blood assay interference in saliva was also tested with Hyris. Results On using TaqPath, SARS-CoV-2 positivity was detected in 35 samples. Another 10 positive samples were artificially-generated by blind mixing of positive with negative samples. Hyris positive and negative percentages of agreement were 97.6 (95% CI: 87.2–99.9%) and 100 (95% CI: 97.0–100%), respectively. Seventeen positive pools, evaluated for threefold strategy, were all correctly determined by both systems. For the 5-pool strategy, 94.7% (18/19) of samples resulted positive with the Hyris system, and 100% with TaqPath. The presence of 1% of blood (v/v) in saliva did not interfere with the accuracy of Hyris assay. Conclusions The sensitivity and specificity of the bKIT Virus Finder COVID-19 Saliva were optimal with respect to TaqPath. In view of the safe and straightforward pre-analytical procedure involved, and the small size of the Hyris bCube, the Hyris system can be used for POCT.

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Comparative Performance of a New SARS-CoV-2 Rapid Detection System

Cited by 9 publications

References 13 publications

Arm race between Rift Valley fever virus and host

Arm race between Rift Valley fever virus and host

Evaluating the efficiency of ELISA, monoplex and multiplex probe‐based real‐time reverse‐transcription PCR assays in the detection of SARS‐CoV‐2 (COVID‐19) and influenza A and B viruses: A cross‐sectional study

Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way

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