Comparative performance of isolation methods using Preston broth, Bolton broth and their modifications for the detection of Campylobacter spp. from naturally contaminated fresh and frozen raw poultry meat

Seliwiorstow, Tomasz; Zutter, Lieven De; Houf, Kurt; Botteldoorn, Nadine; Baré, Julie; Damme, Inge Van

doi:10.1016/j.ijfoodmicro.2016.06.040

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…Other studies have reported Campylobacter contamination rates in poultry meat of between 34.1 to 58.8% ( Giombelli and Gloria, 2014 , Panzenhagen et al., 2016 , Seliwiorstow et al., 2016 , Stella et al., 2017 , Habib et al., 2019 ). Campylobacter contamination reduction is usually expected throughout broiler production steps, from the farm to consumer ( Dogan et al., 2019 ).…”

Section: Resultsmentioning

confidence: 94%

“…The resistance to cefoperazone would explain the spread of P. mirabilis on mCCD agar after enrichment in Bolton broth and the higher efficacy of Preston agar to inhibit contaminant strains sensitive to rifampicin. Owing to the high level of contaminants resistant to third-generation β-lactams used in Campylobacter -selective broth and agar culture media, the addition of rifampicin ( Chon et al., 2017 ), potassium clavulanate ( Moran et al., 2011 , Seliwiorstow et al., 2016 ), or triclosan ( Seliwiorstow et al., 2016 ) to Bolton broth was previously proposed to improve Campylobacter detection in chicken samples. Moreover, it has led to the recent revision of the ISO method for the analysis of samples with low Campylobacter numbers and a high level of background microflora, by introducing the enrichment of the test portions in Preston broth for 22 to 26 h ( Biesta-Peters et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

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Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

et al. 2020

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Chicken is a leading source of thermotolerant Campylobacter , which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobacter contamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48 h after plating onto modified charcoal–cefoperazone–deoxycholate ( mCCD ) agar and Preston agar was assessed. The combined results of the detection and enumeration methods revealed a frequency of 70% occurrence of thermotolerant Campylobacter in chicken samples. Campylobacter was enumerated in 60% of the samples, whereas 46% of the samples were positive in the qualitative analysis. Quantitative analysis showed average counts of 3.10 ± 0.15 log 10 CFU/sample. Higher numbers of Campylobacter -positive samples were found using 24-h enrichment before plating onto Preston agar (46%) than onto mCCD agar (2%). The majority of isolated strains were identified as Campylobacter jejuni , and Campylobacter coli was also found but to a lesser extent. Subtyping revealed a clear distinction between strains isolated from different chicken sources. The enriched samples plated onto mCCD agar showed extensive spreading of nonproducing extended-spectrum β-lactamases Proteus mirabilis that hampered the identification of Campylobacter colonies. P. mirabilis strains showed resistance to cefoperazone, trimethoprim, and polymyxin B present in broth and plate media used and were inhibited by rifampicin present in Preston agar. The results underline the effect of the spread of contaminant strains on Campylobacter cultures, which might be prevented using a recently revised International Organization for Standardization method for qualitative analysis of chicken.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

et al. 2020

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“…The media explored in this research was specifically designed to contain the three most dominant pathophysiological factors present in the wound bed: damaged tissue, red blood cells and plasma. Firstly, BB is a complex desiccated-meat based growth-medium, which contains a high proportion of essential factors that would be present under normal physiological conditions ( Seliwiorstow et al., 2016 ). This importantly includes meat peptones, essential amino acids, salts, and ketones such as sodium pyruvate and alpha-ketoglutaric acid, which have important regulatory functions in cellular metabolic activity and host immunocompetence ( Mathioudakis et al., 2011 ; Glasser et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Bactericidal and anti-biofilm effects of uncharged and cationic ultrasound-responsive nitric oxide microbubbles on Pseudomonas aeruginosa biofilms

LuTheryn

Hind²,

Campbell³

et al. 2022

Front. Cell. Infect. Microbiol.

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Bacterial biofilms are a major and ongoing concern for public health, featuring both inherited genetic resistance traits and a conferred innate tolerance to traditional antibiotic therapies. Consequently, there is a growing need for novel methods of drug delivery, to increase the efficacy of antimicrobial agents. This research evaluated the anti-biofilm and bactericidal effects of ultrasound responsive gas-microbubbles (MBs) of either air or nitric oxide, using an in vitro Pseudomonas aeruginosa biofilm model grown in artificial wound medium. The four lipid-based MB formulations evaluated were room-air MBs (RAMBs) and nitric oxide MBs (NOMBs) with no electrical charge, as well as cationic (+) RAMBs+ and NOMBs+. Two principal treatment conditions were used: i) ultrasound stimulated MBs only, and ii) ultrasound stimulated MBs with a sub-inhibitory concentration (4 µg/mL) of the antibiotic gentamicin. The total treatment time was divided into a 60 second passive MB interaction period prior to 40 second ultrasound exposure; each MB formulation was tested in triplicate. Ultrasound stimulated RAMBs and NOMBs without antibiotic achieved reductions in biofilm biomass of 93.3% and 94.0%, respectively. Their bactericidal efficacy however was limited, with a reduction in culturable cells of 26.9% and 65.3%, respectively. NOMBs with sub-inhibitory antibiotic produced the most significant reduction in biofilm biomass, corresponding to a 99.9% (SD ± 5.21%); and a 99.9% (SD ± 0.07%) (3-log) reduction in culturable bacterial cells. Cationic MBs were initially manufactured to promote binding of MBs to negatively charged biofilms, but these formulations also demonstrated intrinsic bactericidal properties. In the absence of antibiotic, the bactericidal efficacy of RAMB+ and NOMB+ was greater that of uncharged counterparts, reducing culturable cells by 84.7% and 86.1% respectively; increasing to 99.8% when combined with antibiotic. This study thus demonstrates the anti-biofilm and bactericidal utility of ultrasound stimulated MBs, and specifically is the first to demonstrate the efficacy of a NOMB for the dispersal and potentiation of antibiotics against bacterial biofilms in vitro. Importantly the biofilm system and complex growth-medium were selected to recapitulate key morphological features of in vivo biofilms. The results us offer new insight for the development of new clinical treatments, for example, in chronic wounds.

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“…The choice of microbiological method that can be used to isolate campylobacters from a food commodity can significantly affect the prevalence or even the estimated concentration of the pathogen in food [73,[94][95][96][97][98][99][100][101], and therefore the distribution of different Campylobacter species recovered from food during the microorganism's detection or enumeration procedure followed. C. jejuni was mainly isolated during pathogen enumeration procedure, while C. coli was the predominant species recovered from all plating culture media following enrichment in Bolton broth during the detection of Campylobacter in chicken meat [63,98].…”

Section: Detection And/or Enumeration Proceduresmentioning

confidence: 99%

Prevalence and Distribution of Thermotolerant Campylobacter Species in Poultry: A Comprehensive Review with a Focus on the Factors Affecting the Detection and Enumeration of Campylobacter jejuni and Campylobacter coli in Chicken Meat

Andritsos¹,

Tzimotoudis²,

Mataragas³

2023

Preprint

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It is well known that the strong-evidence foodborne outbreaks of human campylobacteriosis are associated with the consumption of raw or incompletely thermally processed poultry meat, whereas broilers act as the main reservoir for Campylobacter species. Campylobacter jejuni and Campylobacter coli are the two main species of campylobacters detected in chicken meat, while they account for almost 90% of the reported cases of campylobacteriosis in humans. Over 80% of these cases are attributed to C. jejuni and about 10% of them are due to C. coli. Therefore, until recently the dominance of C. jejuni against all other Campylobacter spp. isolated from chicken meat samples was well established and unquestionable. Lately, however, C. coli has been increasingly recovered from chicken meat to such an extent that it is now evident that it often comprises the dominant species among the identified campylobacters in the meat samples. This work attempts for the first time a detailed review in the literature to deepen into this noteworthy epidemiological swift in the prevalence of C. jejuni and C. coli, along with the distribution of Campylobacter spp. in chicken meat. Factors such as the sampling method followed for screening campylobacters in broiler carcasses (e.g., swabs or carcass rinsates, skinned or skinless meat excised samples) and part of the animal carcass from which the sample is obtained (e.g., neck, breast, leg), seasonality of sampling (summer vs. winter) and environmental conditions (e.g., rainfall, relative humidity) at the farm level, the isolation procedure (enumeration or detection) and pathogen identification (biochemical or molecular), the enrichment and plating isolation media (e.g., Bolton vs. Preston broth, charcoal-based vs. chromogenic agars), as well as the biofilm-forming ability of different campylobacters, highlight the multivariate dimension of the phenomenon and are thoroughly discussed in the present review.

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Comparative performance of isolation methods using Preston broth, Bolton broth and their modifications for the detection of Campylobacter spp. from naturally contaminated fresh and frozen raw poultry meat

Cited by 15 publications

References 37 publications

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

Bactericidal and anti-biofilm effects of uncharged and cationic ultrasound-responsive nitric oxide microbubbles on Pseudomonas aeruginosa biofilms

Prevalence and Distribution of Thermotolerant <i>Campylobacter</i> Species in Poultry: A Comprehensive Review with a Focus on the Factors Affecting the Detection and Enumeration of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> in Chicken Meat

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