Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles

Abstract

Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activitieswere found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also obtained through chemical composition (FTIR).

Optimization of pineapple flavour synthesis by esterification catalysed by immobilized lipase from Rhizomucor miehei

“…Most of the treatments presented yields of conversion higher than 60% within 24 h of reaction, showing that Lipozyme RM‐IM has a good activity for the synthesis of butyl butyrate. Comparatively, Bruno et al , using an immobilized derivative from R. miehei , obtained 69% of conversion after 24 h of reaction. After optimization of conditions, Santos and Castro, using an immobilized lipase from C. rugosa , obtained a concentration of 0.23 m of butyl butyrate, corresponding to a yield of conversion of 75%.…”

“…Butyl butyrate is in high demand as a natural pineapple flavour by the food, beverage and pharmaceutical industries. There are some reports on the synthesis of butyl butyrate catalysed by lipases and cutinase; among them, the use of lipases from Rhizopus orysae , Mucor miehei , Candida antarctica and Candida rugosa , with yields varying from 69% to 80% of conversion. Concerning the lipase of Rhizomucor miehei , a report on the synthesis of butyl butyrate showed a conversion of 40% obtained after 48 h of reaction.…”

Lipase-Catalyzed Synthesis of Kojic Acid Derivative in Bioreactors and the Analysis of Its Depigmenting and Antioxidant Activities

“…The combination of supercritical fluid and molecular sieves can improve the yield of sugar esters up to 67% [18,21]. In FTR and PBR systems, immobilized M. miehei lipase showed a better operational stability, higher yield, and better reusability performance than C. rugosa lipase after the successive batches of esterification [7,19,22].…”

Anaerobic biodegradability of dairy wastewater pretreated with porcine pancreas lipase

“…Among these, the alternative of using specific enzymes (lipases) has gained more attention because of stringent environmental regulations, clean and friendly enzyme applications (Masse et al, 2001;Mendes et al, 2006a,b;Pereira et al, 2006). Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) are enzymes that catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids (FFA) at the water-lipid interface (Castro et al, 2004;Bruno et al, 2008). These enzymes showed potential applications in degrading the oil and fats in the wastewater generated by dairy industries and slaughterhouses (Masse et al, 2001;Mongkolthanaruk and Dharmsthiti, 2002;Mendes e Castro, 2005;Mendes et al, 2006a,b;Pereira et al, 2006).…”