Comparative pharmacokinetic study of six lignans in normal and diabetic rats after oral administration ofSaururus chinensisextract by LC–MS/MS

Abstract

Saururus chinensis (SC) possesses significant anti-diabetic activity and lignans were its major bioactive compounds. In this study, a rapid and sensitive ultra-highperformance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established for simultaneous quantification of six lignans, namely (-)-(7R,8R)-machilin D (1), verrucesin (2), rel-(7S,8S,7 0 R,8 0 R)-3,3 0 ,4,4 0 ,5,5 0hexamethoxy-7.O.7 0 ,8.8 0 -lignan (3), manassantin A (4), manassantin B (5), and saucerneol F (6) in rat's plasma. It was validated with acceptable linearity (r ≥ 0.9922), accuracy (80.42-95.17%), precision (RSD ≤ 12.08%), and extraction recovery (80.36-93.45%). The method was successfully applied to the comparative pharmacokinetic study of the six lignans in normal and diabetic rats after oral administration of SC extract. Results showed that the areas under the plasma concentration-time curve (AUC 0 ! t and AUC 0 ! ∞ ) of (-)-(7R,8R)-machilin D, rel-(7S,-8S,7 0 R,8 0 R)-3,3 0 ,4,4 0 ,5,5 0 -hexamethoxy-7.O.7 0 ,8.8 0 -lignan, manassantin B, and saucerneol F in diabetic rats were significantly increased, and the plasma clearance (CL) of (-)-(7R,8R)-machilin D in diabetic rats was significantly decreased. However, the AUC 0 ! t and AUC 0 ! ∞ of verrucesin were significantly decreased, and its CL was significantly increased in diabetic rats compared with those in normal rats. These results indicated that there were remarkable differences in the pharmacokinetic parameters between the normal and diabetic rats. The pharmacokinetic studies might be beneficial for the clinical use of SC as hypoglycemic agent. K E Y W O R D S diabetic rats, ignan, pharmacokinetics, Saururus chinensis, UHPLC-MS/MS 1 | INTRODUCTION Saururus chinensis (SC; San Bai Cao in Chinese) has been widely used as a folk medicine in the treatment of edema, beriberi, jaundice, leucorrhea, urinary tract infections, hypertension, hepatitis diseases, and tumors in China, Japan, and South Korea (Meng et al., 2016; Wang et al., 2015). Previous pharmacological studies have shown that SC extract possesses significant anti-diabetic activity through inhibiting alpha-glucosidase activity (Hwang et al., 2007), activating peroxisome proliferator-activated receptor gamma (Hwang et al., 2002), and improving insulin resistance (Guo et al., 2010). In addition, SC extract can inhibit ADP-induced platelet aggregation (He et al., 1992). Phytochemical research studies indicated that SC contains various chemical constituents, such as lignans, flavonoids,

Simultaneous determination of multiple components in rat plasma by UPLC-MS/MS for pharmacokinetic studies after oral administration of Pogostemon cablin extract

“…Notably, most of the current PK studies are in normal animals, and there are fewer in model animals. Numerous studies have shown that disease states may cause significant alterations in PK parameters ( Chen et al, 2022 ; Liu et al, 2022 ; Xiong et al, 2023 ). Since the drugs are mainly used in the pathological state of the body, it is more meaningful to study the PK of TCM in pathological states ( Luo et al, 2014 ).…”

Identification of lignans and quality assessment in Dendrobium officinale under different cultivation modes

“…10,11 However, most of the literature is limited to the structural elucidation and/or bioactivity of isolated compounds or even a single compound. Only a few studies have relied on chromatographic profiling, such as liquid chromatography-mass spectrometry 12,13 or gas chromatography-mass spectrometry 14 or HPLC coupled with multiple-stage tandem mass spectrometry using electrospray ionization (HPLC-ESI-MS n ), 15,16 to obtain more comprehensive results, but numerous minor constituents remained undetected. Thus, the data on the detailed chemical profiles and relative abundances of lignans in Dendrobium are still lacking.…”