Comparative pharmacokinetics of 11 components from the active part of Gerberae Piloselloidis Herba after oral administration in control and asthmatic mice

Abstract

Gerberae Piloselloidis Herba, a traditional Chinese medicine, is often employed to treat such lung‐related diseases as coughs, asthma, and pulmonary carbuncles in southwest China. Our previous study demonstrated that its active fraction, prepared from Gerberae Piloselloidis Herba, exerts an obvious beneficial effect on asthma. However, the pharmacokinetics of its major constituents remain unclear. Therefore, an ultra‐performance mass spectrometry‐electrospray ionization‐tandem mass spectrometry method was successfully established to simultaneously perform the pharmacokinetics of the main 11 components of the active fraction between normal and ovalbumin‐induced asthmatic mice. Compared to the normal group, in asthmatic mice the peak concentration of arbutin, marmesin, caffeoylquinic acids, and flavonoid glycosides clearly increased, while for luteolin it significantly declined; the area under the curve for arbutin and luteolin showed an increase, but the values of marmesin, caffeoylquinic acids, and flavonoid glycosides revealed a decline; the peak time for arbutin, caffeoylquinic acids and flavonoid glycosides decreased, while for marmesin and luteolin it significantly augmented; apart from marmesin, the half‐life for all compounds shortened significantly. It is indicated that the pathology of asthma could lead to an alteration in the pharmacokinetic profiles of the 11 components in plasma, providing a reference for further exploration of the pharmacodynamic basis of the anti‐bronchial effect of Gerberae Piloselloidis Herba.

Comparative pharmacokinetic studies of Ephedra herba in common cold and nephrotic syndrome rat models

“…Traditional Chinese medicine is a complex system containing a variety of components, only the components with significant exposure and strong pharmacodynamic activity in vivo could have a good chance of being effective substances [1]. Pharmacokinetics is the study of blood/drug concentrations over time and is of importance in determining the dynamics of absorption, distribution, metabolism, and excretion of active ingredients in the body [2]. Therefore, the pharmacokinetic study is an effective method for predicting effective substances and explaining their effective mechanisms.…”

Simultaneous determination of cordycepin and its metabolite 3′‐deoxyinosine in rat whole blood by ultra‐high‐performance liquid chromatography coupled with Q Exactive hybrid quadrupole orbitrap high‐resolution accurate mass spectrometry and its application to accurate pharmacokinetic studies

“…In addition, the lower LOQ (LLOQ) for the analysis method has to be low enough to quantify the major metabolites of COR in the pharmacokinetic study sufficiently. Ultra-HPLC coupled with Q Exactive hybrid quadrupole orbitrap high-resolution accurate MS (UHPLC-Q-Orbitrap HRMS) exhibits good specificity, high sensitivity, and high mass resolution and has attracted increasing attention in the bioanalysis and pharmacokinetic study [19,20].…”