Comparative pharmacokinetics of four major flavonoids in normal and chronic gastritis rats after oral administration of different combinations of Banxia Xiexin decoction

Abstract

Chronic gastritis (CG) has become a major threat to human health. Banxia Xiexin Decoction (BXXXD) has been used clinically to treat gastritis by acting on the spleen and stomach for thousands of years. Baicalin, wogonoside, liquiritin and liquiritigenin are the main bioactive flavonoids of BXXXD. A rapid, sensitive and selective HPLCtriple quadrupole (TQ)-MS/MS method was developed to simultaneously quantify the four flavonoids in rat plasma in this study. With salidroside as internal standard (IS), plasma samples were extracted and separated on a Welch HPLC XB-C 18 column (2.1 Â 50 mm, 1.8 μm) using gradient elution. The optimized gradient of the mobile phase consisted of water (containing 0.1% formic acid) (A) and methanol (B) was used.Detection was implemented in multiple reaction monitoring mode with an electrospray negative ionization source. The comparative pharmacokinetics of four analytes in normal and CG rats after oral administration of BXXXD or its different compatibilities were first investigated. The results indicated that the pharmacokinetic behaviors of analytes were obviously changed in CG rats. From the comparison between the whole prescription group and the compatibility groups, it was found that the pharmacokinetic behavior of analytes also changed to some extent. The pharmacokinetic alterations of analytes might be due to the pathological conditions of CG.

Metabolite profiling of liquiritin in acute myocardial infarction model rat after intragastric administration using an information‐dependent acquisition‐mediated ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method

“…Some metabolites of HCl conjugate might be transformed from LQ under the action of gastric acid after intragastric administration. LQ reached C max within 1 h, which was in good agreement with those from previous work (Wan et al, 2020; Wang et al, 2021; Zhou et al, 2022). Compared with the peak area–time curves, the metabolites of LQ reached C max within 5 h and were eliminated slowly, which explained the poor absorption and rapid elimination of LQ.…”

“…Some metabolites of HCl conjugate might be transformed from LQ under the action of gastric acid after intragastric administration. LQ reached C max within 1 h, which was in good agreement with those from previous work(Wan et al, 2020;Wang et al, 2021;Zhou et al, 2022). Compared with the F I G U R E 4 Proposed metabolic pathways for liquiritin (LQ) in acute myocardial infarction (AMI) rats.…”

Banxia Xiexin decoction: A review on phytochemical, pharmacological, clinical and pharmacokinetic investigations

“…Recent research by Zhou et al revealed systematic PK data of BXD under the pathological settings of chronic gastritis. [ 126 ] In comparison to the control group, the PK parameters of baicalin, wogonoside, liquiritin, and liquiritigenin in rats with chronic gastritis altered dramatically, with significant reductions in C max , AUC (0-t) , and AUC (0-∞) . The T 1/2 of wogonoside and liquiritin were also considerably decreased in rats with chronic gastritis.…”