Comparative pharmacology of two D1-like dopamine receptors cloned from the silkworm Bombyx mori

Ohta, Hiroto; Tsuchihara, Kazuko; Mitsumasu, Kanako; Yaginuma, Toshinobu; Ozoe, Yoshihisa; Asaoka, Kiyoshi

doi:10.1016/j.ibmb.2009.01.011

Cited by 25 publications

(22 citation statements)

References 46 publications

(52 reference statements)

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“…This phenomenon was not reported in Drosophila b-adrenergic receptors (Balfanz et al, 2005;Maqueira et al, 2005), and it is interesting to note that a similar biphasic response in cAMP production was observed for the same two agonists in experiments with the light organs of fireflies (Nathanson, 1985) and BmOAR1 expressed in HEK-293 cells (Huang et al, 2010). A B. mori DA receptor (BmDopR1) expressed in HEK-293 cells also displayed a biphasic response to DA (Ohta et al, 2009). This distinguishing phenomenon might indicate that BmOAR2 is desensitized by high concentrations of agonists, as in the case of b-adrenergic receptors (Daaka et al, 1997;Hausdorff et al, 1990).…”

Section: Discussionmentioning

confidence: 55%

Functional and pharmacological characterization of a β-adrenergic-like octopamine receptor from the silkworm Bombyx mori

Chen

Ohta

Ozoe

et al. 2010

Insect Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 55%

Functional and pharmacological characterization of a β-adrenergic-like octopamine receptor from the silkworm Bombyx mori

Chen

Ohta

Ozoe

et al. 2010

Insect Biochemistry and Molecular Biology

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“…8C) at 10 Ϫ6 M and tested the ability of moths to form an aversive association between the Datura odor and the simulated toxic nectar (see below). SCH23390 (Tocris) was selected for its effectiveness in blocking the Dop1-and INDR-type receptors in the silk moth Bombyx mori (Ohta et al 2009). Because of the high degree of sequence similarity for the DA receptors between Manduca and Bombyx, SCH23390 is likely an effective Dop1-and INDR-type receptor antagonist in Manduca.…”

Section: Methodsmentioning

confidence: 99%

Olfactory modulation by dopamine in the context of aversive learning

Dacks

Riffell

Martin

et al. 2012

Journal of Neurophysiology

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The need to detect and process sensory cues varies in different behavioral contexts. Plasticity in sensory coding can be achieved by the context-specific release of neuromodulators in restricted brain areas. The context of aversion triggers the release of dopamine in the insect brain, yet the effects of dopamine on sensory coding are unknown. In this study, we characterize the morphology of dopaminergic neurons that innervate each of the antennal lobes (ALs; the first synaptic neuropils of the olfactory system) of the moth Manduca sexta and demonstrate with electrophysiology that dopamine enhances odor-evoked responses of the majority of AL neurons while reducing the responses of a small minority. Because dopamine release in higher brain areas mediates aversive learning we developed a naturalistic, ecologically inspired aversive learning paradigm in which an innately appetitive host plant floral odor is paired with a mimic of the aversive nectar of herbivorized host plants. This pairing resulted in a decrease in feeding behavior that was blocked when dopamine receptor antagonists were injected directly into the ALs. These results suggest that a transient dopaminergic enhancement of sensory output from the AL contributes to the formation of aversive memories. We propose a model of olfactory modulation in which specific contexts trigger the release of different neuromodulators in the AL to increase olfactory output to downstream areas of processing.

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“…Orthologues of this receptor have subsequently been described in several arthropod species [10], [11], [12], [13], including in honey bees ( Am DOP2, [7]). All of the invertebrate-type DA receptors examined so far have been found to be positively coupled to adenylyl cyclase and hence are often described as D1-like DA receptors [1].…”

Section: Introductionmentioning

confidence: 99%

Honey Bee Dopamine and Octopamine Receptors Linked to Intracellular Calcium Signaling Have a Close Phylogenetic and Pharmacological Relationship

2011

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BackgroundThree dopamine receptor genes have been identified that are highly conserved among arthropod species. One of these genes, referred to in honey bees as Amdop2, shows a close phylogenetic relationship to the a-adrenergic-like octopamine receptor family. In this study we examined in parallel the functional and pharmacological properties of AmDOP2 and the honey bee octopamine receptor, AmOA1. For comparison, pharmacological properties of the honey bee dopamine receptors AmDOP1 and AmDOP3, and the tyramine receptor AmTYR1, were also examined.Methodology/Principal FindingsUsing HEK293 cells heterologously expressing honey bee biogenic amine receptors, we found that activation of AmDOP2 receptors, like AmOA1 receptors, initiates a rapid increase in intracellular calcium levels. We found no evidence of calcium signaling via AmDOP1, AmDOP3 or AmTYR1 receptors. AmDOP2- and AmOA1-mediated increases in intracellular calcium were inhibited by 10 µM edelfosine indicating a requirement for phospholipase C-β activity in this signaling pathway. Edelfosine treatment had no effect on AmDOP2- or AmOA1-mediated increases in intracellular cAMP. The synthetic compounds mianserin and epinastine, like cis-(Z)-flupentixol and spiperone, were found to have significant antagonist activity on AmDOP2 receptors. All 4 compounds were effective antagonists also on AmOA1 receptors. Analysis of putative ligand binding sites offers a possible explanation for why epinastine acts as an antagonist at AmDOP2 receptors, but fails to block responses mediated via AmDOP1.Conclusions/SignificanceOur results indicate that AmDOP2, like AmOA1, is coupled not only to cAMP, but also to calcium-signalling and moreover, that the two signalling pathways are independent upstream of phospholipase C-β activity. The striking similarity between the pharmacological properties of these 2 receptors suggests an underlying conservation of structural properties related to receptor function. Taken together, these results strongly support phylogenetic analyses indicating that the AmDOP2 and AmOA1 receptor genes are immediate paralogs.

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Comparative pharmacology of two D1-like dopamine receptors cloned from the silkworm Bombyx mori

Cited by 25 publications

References 46 publications

Functional and pharmacological characterization of a β-adrenergic-like octopamine receptor from the silkworm Bombyx mori

Functional and pharmacological characterization of a β-adrenergic-like octopamine receptor from the silkworm Bombyx mori

Olfactory modulation by dopamine in the context of aversive learning

Honey Bee Dopamine and Octopamine Receptors Linked to Intracellular Calcium Signaling Have a Close Phylogenetic and Pharmacological Relationship

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