Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods

Wittkopp, Jeremy; Khoo, Ting Chean; Carney, Shane; Pisila, Kai; Bahreini, Shahab J; Tubbesing, Kate; Mahajan, Supriya D.; Petruccelli, Jonathan C.; Khmaladze, Alexander

doi:10.1364/oe.385854

Cited by 23 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The question then shifts to how to measure CV. A number of approaches are available, with various degrees of compatibility with QPI [6,35]; our preferred QPI/CV combination is the transmission-through-dye (TTD) method for CV [36,37] and transport-of-intensity equation (TIE) imaging for QPI [18,38]; both can be readily realized on a standard optical transmission microscope and allow simultaneous fluorescence imaging.…”

Section: Quantification Of Macromolecular Crowding In Living Cellsmentioning

confidence: 99%

Macromolecular Crowding: a Hidden Link Between Cell Volume and Everything Else

Model

Hollembeak

Kurokawa

2021

Cell Physiol Biochem

View full text Add to dashboard Cite

High density of intracellular macromolecules creates a special condition known as macromolecular crowding (MC). One well-established consequence of MC is that only a slight change in the concentration of macromolecules (e.g., proteins) results in a shift of chemical equilibria towards the formation of macromolecular complexes and oligomers. This suggests a physiological mechanism of converting cell density changes into cellular responses. In this review, we start by providing a general overview of MC; then we examine the available experimental evidence that MC may act as a direct signaling factor in several types of cellular activities: mechano- and osmosensing, cell volume recovery in anisosmotic solutions, and apoptotic shrinkage. The latter phenomenon is analyzed in particular detail, as persistent shrinkage is known both to cause apoptosis and to occur during apoptosis resulting from other stimuli. We point to specific apoptotic reactions that involve formation of macromolecular complexes and, therefore, may provide a link between shrinkage and downstream responses.

show abstract

Section: Quantification Of Macromolecular Crowding In Living Cellsmentioning

confidence: 99%

Macromolecular Crowding: a Hidden Link Between Cell Volume and Everything Else

Model

Hollembeak

Kurokawa

2021

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…In the works by Zuo et al, TIE was invoked following the numerical reconstruction and propagation of the digital hologram, and the absolute phase without 2π discontinuities has been directly recovered (Zuo et al, 2013b). Whittkopp et al described a microscopic setup implementing phase imaging by DHM and TIE, which allowed the results of both measurements to be quantitatively compared for either live cell or static samples (Wittkopp et al, 2020). Gupta et al combined TIE with DH to overcome the artifacts caused by TIE phase recovery under low-light conditions by reconstructing the desired multiple out-of-focus intensity maps from the captured coaxial digital holograms (Gupta et al, 2020;Gupta and Nishchal, 2021).…”

Section: Introductionmentioning

confidence: 99%

Elimination of Quadratic Phase Aberration in Digital Holographic Microscopy by Using Transport of Intensity

et al. 2022

View full text Add to dashboard Cite

We propose to reconstruct 3D images by combining the merits of transport of intensity and digital holography. The proposed method solves the transport-of-intensity equation by using digital holographic reconstructed images as inputs. Our simulation and experimental results show that this method can eliminate quadratic phase aberration introduced by the microscope objective in digital holographic microscopy. This proposed phase retrieval method is free of phase unwrapping process. It is thus efficient in removing quadratic phase aberration introduced by the microscope objective.

show abstract

“…Further, Zhou et al [29] proposed a technique, in which intensity images reconstructed from a DH were used as input for TIE for achieving unwrapped phase recovery. Phase imaging through DH and TIE was compared [30] for live cells and it was recommended to be combined for better accuracy. Of late, the combination has been reported [30][31][32][33][34][35] with better phase imaging capabilities and has been applied to varied types of applications.…”

Section: Introductionmentioning

confidence: 99%

“…Phase imaging through DH and TIE was compared [30] for live cells and it was recommended to be combined for better accuracy. Of late, the combination has been reported [30][31][32][33][34][35] with better phase imaging capabilities and has been applied to varied types of applications.…”

Section: Introductionmentioning

confidence: 99%

Combining digital holography and transport of intensity equation for quantitative phase imaging: a review

Kumar¹,

Nishchal²,

Singh³

2022

AJP

View full text Add to dashboard Cite

Dedicated to Professor Partha Banerjee for his enormous contributions to the advancement of research and education in holography through his unique vision and outstanding dedicationRecent advances in the field of digital sensors and computational facilities have turned digital holography (DH) into a powerful tool for many applications such as quantitative phase imaging, optical metrology, evaluation of cell parameters, optical cryptography, and optical pattern recognition among others. The transport of intensity equation (TIE) is one of the deterministic phase-retrieval methods, which does not use interferometric geometry. In this method, the phase is calculated directly from a set of intensities rather than iteratively approximating a solution. Recently, use of the TIE in the DH reconstruction process has been reported as a phase-retrieval method. Combining both DH and TIE provides better solution to the phase-retrieval. Refocusing property of the DH helps exploit the translation issue of digital sensor along the optical axis while capturing the intensity distributions at different depths. The issue of phase unwrapping is solved through the use of TIE. Hence, both the methods complement each other. In this paper, we review briefly the applicability of the combination of DH with TIE.

show abstract

Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods

Cited by 23 publications

References 47 publications

Macromolecular Crowding: a Hidden Link Between Cell Volume and Everything Else

Macromolecular Crowding: a Hidden Link Between Cell Volume and Everything Else

Elimination of Quadratic Phase Aberration in Digital Holographic Microscopy by Using Transport of Intensity

Combining digital holography and transport of intensity equation for quantitative phase imaging: a review

Contact Info

Product

Resources

About