Comparative phosphoproteomic provides insights into meat quality differences between slow- and fast-growing broilers

Weng, Kaiqi; Li, Yi; Huo, Weiran; Cao, Zhen; Zhang, Yang; Xu, Qi

doi:10.1016/j.foodchem.2021.131408

Cited by 27 publications

(17 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the identified phosphorylation sites, the majority occur at serine (S) residues, accounting for 73.27% of the sites, followed by threonine (T) (22.52%) and tyrosine (T) (4.21%) ( Figure 2A ). This distribution of phosphorylation across these three amino acid residues is consistent with those identified in sheep ( 33 ), yak ( 18 ), chicken ( 34 ), and geese ( 16 ). These data suggest that the amino acid residues modified by phosphorylation are relatively similar in different animals.…”

Section: Resultssupporting

confidence: 85%

“…The major serine phosphorylation motifs were P×SP, RR×S, R××SP, SP×××R, SPP, RS×S, SP, R××S, R×S, GS, S×××××K, K××S, S××K, and S××E, while the threonine phosphorylation motif was TP, where × is any residue ( Figure 2C ). The above motifs were also observed in a phosphoproteomic analysis of broiler chicken proteins ( 34 ), suggesting that, as expected, phosphorylation modification sites are conserved across animals. A heatmap analysis of the preferences for each of these 15 amino acid sequence motifs revealed that arginine was the preferred amino acid upstream of the phosphorylated serine or threonine residue ( Figures 2D, E ), while proline was preferred to be located downstream ( Figures 2D, E ).…”

Section: Resultssupporting

confidence: 69%

See 1 more Smart Citation

Phosphoproteomic analysis identifies differentially expressed phosphorylation sites that affect muscle fiber type in pigs

Tan

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Skeletal muscle of livestock is composed of both fast- and slow-twitch muscle fibers, which are key factors in their meat quality. However, the role of protein phosphorylation in muscle fiber type is not completely understood. Here, a fast-twitch (biceps femoris, BF) and slow-twitch (soleus, SOL) muscle tissue sample was collected from three male offspring of Duroc and Meishan pigs. We demonstrate that the meat quality of SOL muscle is significantly better than that of BF muscle. We further used phosphoproteomic profiling of BF and SOL muscles to identify differences between these muscle types. A total of 2,327 phosphorylation sites from 770 phosphoproteins were identified. Among these sites, 287 differentially expressed phosphorylation sites (DEPSs) were identified between BF and SOL. GO and KEGG enrichment analysis of proteins containing DEPSs showed that these phosphorylated proteins were enriched in the glycolytic process GO term and the AMPK signaling pathway. A protein-protein interaction (PPI) analysis reveals that these phosphorylated proteins interact with each other to regulate the transformation of muscle fiber type. These analyses reveal that protein phosphorylation modifications are involved in porcine skeletal muscle fiber type transformation. This study provides new insights into the molecular mechanisms by which protein phosphorylation regulates muscle fiber type transformation and meat quality in pigs.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Resultssupporting

confidence: 69%

Phosphoproteomic analysis identifies differentially expressed phosphorylation sites that affect muscle fiber type in pigs

Tan

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…SNAP25, BLZF1, EXOC6B, and EHD3 participate in vehicle docking during exocytosis. In addition, AUP1 , which is located on GGA4, participates in lipid metabolism and has a positive effect on intramuscular fat deposition, and AUP1, a CUE domain protein, participates in lipid droplet formation and induces lipid droplet aggregation, increasing lipid deposition in TEY and improving TEY quality ( Weng et al, 2022 ). UBE2B is located on GGA13 and participates in lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate genomic regions for thermogelled egg yolk traits based on a genome-wide association study

Zhang¹,

Li²,

Ma³

et al. 2023

Poultry Science

View full text Add to dashboard Cite

“…Chicken meat is the second-largest meat product marketed for human consumption worldwide due to its taste, tenderness, and juicy quality. With the use of continuous growth breeding and high-density feeding to obtain more meat, the quality of the meat decreases due to the resulting relatively higher water and lower fat content [ 1 ]. At present, improving meat quality is the focus of intensive scientific research.…”

Section: Introductionmentioning

confidence: 99%

SLC16A7 Promotes Triglyceride Deposition by De Novo Lipogenesis in Chicken Muscle Tissue

Wang

Liu

et al. 2022

Biology

View full text Add to dashboard Cite

Triglyceride (TG) content in chicken muscle tissue signifies intramuscular fat (IMF) content, which is important for improving meat quality. However, the genetic basis of TG deposition in chicken is still unclear. Using 520 chickens from an artificially selected line with significantly increased IMF content and a control line, a genome-wide association study (GWAS) with TG content reports a region of 802 Kb located in chromosome 1. The XP-EHH and gene expression analysis together reveal that the solute carrier family 16 member A7 (SLC16A7) gene is the key candidate gene associated with TG content in chicken muscle tissue. Furthermore, the weighted gene co-expression network analysis (WGCNA) confirmed the regulatory effects of SLC16A7 on promoting TG deposition by de novo lipogenesis (DNL). Functional verification of SLC16A7 in vitro also supports this view, and reveals that this effect mainly occurs in myocytes. Our data highlight a potential IMF deposition pathway by DNL, induced by SLC16A7 in chicken myocytes. These findings will improve the understanding of IMF regulation in chicken and guide the formulation of breeding strategies for high-quality chicken.

show abstract

Comparative phosphoproteomic provides insights into meat quality differences between slow- and fast-growing broilers

Cited by 27 publications

References 33 publications

Phosphoproteomic analysis identifies differentially expressed phosphorylation sites that affect muscle fiber type in pigs

Phosphoproteomic analysis identifies differentially expressed phosphorylation sites that affect muscle fiber type in pigs

Identification of candidate genomic regions for thermogelled egg yolk traits based on a genome-wide association study

SLC16A7 Promotes Triglyceride Deposition by De Novo Lipogenesis in Chicken Muscle Tissue

Contact Info

Product

Resources

About