Comparative physical, chemical and biological assessment of simple and titanium-doped ovine dentine-derived hydroxyapatite coatings fabricated by pulsed laser deposition

Duta, L.; Mihăilescu, Natalia; Popescu, Andrei C.; Luculescu, C.; Mihăilescu, I. N.; Çetin, Gözde Yıldırım; Gündüz, Oğuzhan; Oktar, Faik N.; Popa, Ana Maria; Kuncser, Andrei; Beșleagă, Cristina; Stan, George E.

doi:10.1016/j.apsusc.2017.04.025

Cited by 59 publications

(60 citation statements)

References 36 publications

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“…(i) The first one, which is generally utilized due its reliability, implies the use of inorganic synthesis by different methods, such as hydrothermal [20,21], co-precipitation [22,23], or sol-gel [24]. However, these approaches are, on one hand, polluting and time-consuming, and, on the other hand, quite expensive [25,26]. Moreover, the resulting synthetic HA, which is a stoichiometric material with Ca/P ratio of 1.67, does not completely match the chemical composition of bone [27].…”

Section: Bioha Vs Synthetic Hamentioning

confidence: 99%

“…For the crystallites size function of dopant materials in the case of BioHA coatings synthesized by PLD, the reader is guided to consult Table 2. Duta et al [25] observed that a percentage of 1.5 wt.% Ti in composition of PLD targets from SHA causes synthesis of layers with a reduced degree of crystallinity. Deposited SHA coatings without Ti display a crystallinity degree of~30%, while, for the ones doped with Ti, deposited in the same experimental conditions, the crystallinity degree dropped to 7% only.…”

Section: Bioha Vs Synthetic Hamentioning

confidence: 99%

“…After washing and drying, for the calcination process, the bone pieces are heated in a furnace (at various temperatures ranging from 350 • C to 1400 • C, for 1 to 18 h in air (Table 3), in order to completely remove the organic matter from the bones. One should emphasize that the as-prepared powders are biologically safe due to the high temperature fabrication route, which not only favors the crystallization of the material, but also completely prevents any risk of diseases transmission, since no pathogen can survive to such extreme conditions [25,53,54,60]. The resulting calcined bone specimens are cooled to room temperature (RT) by slow furnace cooling.…”

Section: Extraction Of Ha From Mammalian Bonesmentioning

confidence: 99%

“…The powders obtained following the protocols mentioned in Section 4 are generally pressed and the resulting pellets are heat-treated in a furnace, to reach compactness by eliminating air bubbles and water vapors. The as-sintered pellets are compact and tough ( Figure 1) and can be used as targets in the PLD experiments [11,25,53,54,60,80].…”

Section: Bioha Targets Preparationmentioning

confidence: 99%

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Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Duta

Popescu

2019

Coatings

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The aim of this paper is to present the current status on animal-origin hydroxyapatite (HA) coatings synthesized by Pulsed Laser Deposition (PLD) technique for medical implant applications. PLD as a thin film synthesis method, although limited in terms of surface covered area, still gathers interest among researchers due to its advantages such as stoichiometric transfer, thickness control, film adherence, and relatively simple experimental set-up. While animal-origin HA synthesized by bacteria or extracted from animal bones, eggshells, and clams was tested in the form of thin films or scaffolds as a bioactive agent before, the reported results on PLD coatings from HA materials extracted from natural sources were not gathered and compared until the present study. Since natural apatite contains trace elements and new functional groups, such as CO32− and HPO42− in its complex molecules, physical-chemical results on the transfer of animal-origin HA by PLD are extremely interesting due to the stoichiometric transfer possibilities of this technique. The points of interest of this paper are the origin of HA from various sustainable resources, the extraction methods employed, the supplemental functional groups, and ions present in animal-origin HA targets and coatings as compared to synthetic HA, the coatings’ morphology function of the type of HA, and the structure and crystalline status after deposition (where properties were superior to synthetic HA), and the influence of various dopants on these properties. The most interesting studies published in the last decade in scientific literature were compared and morphological, elemental, structural, and mechanical data were compiled and interpreted. The biological response of different types of animal-origin apatites on a variety of cell types was qualitatively assessed by comparing MTS assay data of various studies, where the testing conditions were possible. Antibacterial and antifungal activity of some doped animal-origin HA coatings was also discussed.

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Section: Bioha Vs Synthetic Hamentioning

confidence: 99%

Section: Bioha Vs Synthetic Hamentioning

confidence: 99%

Section: Extraction Of Ha From Mammalian Bonesmentioning

confidence: 99%

Section: Bioha Targets Preparationmentioning

confidence: 99%

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Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Duta

Popescu

2019

Coatings

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“…The femoral shafts were further cut into slices, cleaned and washed with distilled water and deproteinized for 14 days in an alkali media of 1% sodium hypochlorite. The resulting dry bone fragments were submitted to a calcination process (at 850 • C, 4 h in air), to remove any residual organic or biological hazardous components [27,28]. Before undergoing ball-milling to fine powders, the calcined bone specimens were crushed with a mortar and pestle.…”

Section: Powders Preparationmentioning

confidence: 99%

Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

et al. 2019

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This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.

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Nanofibers of polycaprolactone encapsulated with Cu/vanadate hydroxyapatite for wound dressing applications: mechanical and in vitro cells adhesion

Alkhamisi

Almotiri

2022

Appl. Phys. A

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Comparative physical, chemical and biological assessment of simple and titanium-doped ovine dentine-derived hydroxyapatite coatings fabricated by pulsed laser deposition

Cited by 59 publications

References 36 publications

Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

Nanofibers of polycaprolactone encapsulated with Cu/vanadate hydroxyapatite for wound dressing applications: mechanical and in vitro cells adhesion

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