Comparative Physico-chemical and in Vitro Properties of Fibrillated Collagen Scaffolds from Different Sources

Abstract

Collagen from different sources was isolated and designed as scaffolds to act as a three-dimensional substrate for culturing human skin fibroblasts, which can be used as dermal substitutes. The thermodynamic behavior of the scaffolds developed was analyzed through Differential Scanning Calorimetric (DSC) and Thermogravimetric analysis (TGA). Analysis by Fourier Transform Infrared Spectroscopy (FTIR) revealed the functional groups in the scaffolds and the mechanical stability of various scaffolds was assessed through tensile strength analysis. Human skin fibroblasts were cultured on the developed scaffolds to assess their cellular interaction and behavior, and the morphological characteristics of the cultured fibroblasts were evaluated using Scanning Electron Microscopy (SEM). The collagen scaffold exhibited unique features when developed from various sources and it was observed that cells could grow and proliferate well and spread as a monolayer in the reconstituted collagen scaffold.

Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications

“…, Young's modulus, ultimate tensile strength, stiffness, and percentage of elongation of the dried scaffold materials, were measured using a universal testing machine (model 1405, Instron Corporation, Norwood, MA, USA) at a crosshead speed of 5 mm min -1 at 25°C and 65% relative humidity. Length and width of the dumbbell-shaped test samples were maintained at 20 and 5 mm, respectively, according to Shanmugasundaram et al (2004). All the mechanical tests were performed with dried samples and were examined in triplicates.…”

Effect of curcumin caged silver nanoparticle on collagen stabilization for biomedical applications

“…The improved stability of triple helical structure due to cross-linking leads to a low denaturation rate of collagen and higher melting temperature [10,58,59].…”

Controlled release of 2, 3 desulfated heparin exerts its anti‐inflammatory activity by effectively inhibiting E‐selectin

“…As the primary intent of designing a controlled delivery system for 2, 3 DSH is to target inflammatory status during wound healing; the results are validated using collagen membrane as a model system. Based on our earlier investigations scaffold prepared from bovine skin collagen was used as it possess characteristics to act as an ideal wound cover 11. Further, the methodology developed in this study gives the fabrication of a delivery system that can respond to the changes in the local environment as the dressing was developed as a drug delivery carrier and also as a wound cover.…”