Comparative Physicochemical Characterization and Bioavailability of Nano-Liposomes Formed by Mixing Lipids at Different Ratios

Abstract

Liposomes are small lipid vesicles that mimic biological membranes and have been spotlighted in the clinical field due to their ability to enclose a biologically active substance of any structure and to release it into the host's body. This study compares the physicochemical properties and biological activity of nano-liposomes with different compositions to determine the most effective formulation for further in vivo application. Nano-scale liposomes composed of different ratios of 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), dihexadecyl phosphate (DCP), and cholesterol (Chol): DMPE, DMPE/DCP, DMPE/Chol, and DMPE/DCP/Chol were produced. The thermal phase transition was assessed via differential scanning calorimetry (DSC); the particle size, via dynamic light scattering (DLS); the colloidal stability, via the zeta potential; the direct morphological characterization, via transmission electron microscopy (TEM); and the protein encapsulation efficiency. The bioavailability was also investigated with respect to the immunological responses via porcine interferon gamma (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay in peripheral blood mononuclear cells (PBMC) of immunocompetent pigs. All the liposomes can be expected to be stable in an in vivo physiological temperature, and the liposomes that were prepared from DMPE/DCP showed the best efficiency in the in vitro model that mimicked the release of a bioactive substance in vivo. In the result of DLS and the zeta potential for the investigation of the colloidal stability in the system, DMPE/DCP/Chol appeared better than the other formulations. The porcine IFN-gamma ELISPOT assay results postulated that DMPE/DCP most potently induced the IFN-gamma secretion by PBMC, followed by DMPE/DCP/Chol and DMPE alone, in that order.

Nanoliposome Powder Containing Shrimp Oil Increases Free Flowing Behavior and Storage Stability

“…This affects the van der Waals interactions between the hydrocarbon chains and increases their mobility. [ 62 ] Most of the phospholipid bilayers have a transition temperature between 40 and 70 °C. [ 63 ] SONL prepared by ultrasonication method had a T g of 57.3 °C.…”

Theranostics Aspects of Various Nanoparticles in Veterinary Medicine

“…The immunological responses were examined by using the newly formed nano-liposomes via porcine interferon gamma (IFN-γ) enzyme-linked immunospot (ELISPOT) assay in peripheral blood mononuclear cells (PBMC) of immunocompetent pigs. The porcine IFN-γ ELISPOT assay results revealed that DMPE/DCP most potently induced the IFN-γ secretion by PBMC, followed by DMPE/DCP/Chol and DMPE [ 27 ]. Liposomes based on phospholipids used as adjuvant and adjuvant carrier in various microbial diseases caused by different type of microorganisms, including P. malarie [ 28 ], Influenza Virus (INFLEXAL ® V) [ 29 ], HAV (Epaxal ® ) HIV [ 30 ], and ICMV P. vivax [ 28 ].…”

Extracting Signatures of Spatial Organization for Biomolecular Nanostructures

“…[30][31][32][33][34][35][36][37][38] Elucidation of actual structural features of such small assemblies, especially smaller than 100 nm and more specifically in the sub-10 nm scales, has been possible (to some extent) by development of analytical tools for extracting geometrical signatures from indirect data measurements (e.g., molecular diffraction patterns 7 27 and application of techniques such as fluorescence resonance energy transfer 39 ). In general, pattern generation and recognition in 3-D space have been of wide interest in the area of molecular structures.…”