Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

Abstract

We investigated the physiological and proteomic changes in the leaves of three Lolium perenne genotypes, one Iranian putative self-pollinating genotype named S10 and two commercial genotypes of Vigor and Speedy, subjected to drought stress conditions. The results of this study indeed showed higher RWC (relative water content), SDW (shoot dry weight), proline, ABA (abscisic acid), nitrogen and amino acid contents, and antioxidant enzymes activities of S10 under drought stress in comparison with the two other genotypes. A total of 915 proteins were identified using liquid chromatography-mass spectrometry (LC/MS) analysis, and the number of differentially abundant proteins between normal and stress conditions was 467, 456, and 99 in Vigor, Speedy, and S10, respectively. Proteins involved in carbon and energy metabolism, photosynthesis, TCA cycle, redox, and transport categories were up-regulated in the two commercial genotypes. We also found that some protein inductions, including those involved in amino acid and ABA metabolisms, aquaporin, HSPs, photorespiration, and increases in the abundance of antioxidant enzymes, are essential responses of the two commercial genotypes to drought stress. In contrast, we observed only slight changes in the protein profile of the S10 genotype under drought stress. Higher homozygosity due to self-pollination in the genetic background of the S10 genotype may have led to a lower variation in response to drought stress conditions.

Perennial Ryegrass Contains Gluten-Like Proteins That Could Contaminate Cereal Crops

“…The protein search database comprised the public sequences of the Poaceae family proteins from UniProt, a higher taxonomic group for wheat and ryegrass representing diverse genetic variations. Previous proteomics studies in L. perenne have used a different database approach where Brachypodium distachyon sequences were searched to successfully characterise drought response in the leaves from this grass ( 25 ); because of the focus on this compartment, no gluten-like proteins were identified in this study. No other studies besides Colgrave et.al.…”

Spermine: Its Emerging Role in Regulating Drought Stress Responses in Plants

“…Drought is a major global threat to farming as the resulting stress severely alters key physiological and developmental processes [ 20 , 21 , 22 , 23 , 24 , 25 ], reducing production by as much as 25% [ 26 ]. Long-term drought leads to physiological and metabolic changes in plants including loss of cell turgor, water and mineral imbalances, and photosynthetic abnormalities [ 27 ].…”

Screening of Iraqi barley accessions under PEG-induced drought conditions

“…Consequently, one of the best approaches to study the effects of drought stress on germination, is to create conditions for drought stress using different osmotic materials to generate osmotic capacities. In these respects, polyethylene glycol (PEG-6000) is used specifically for assessing details relevant to dryness tension from plants (Veselov et al 2002;Vanani et al 2020). This molecule has a high molecular weight; it cannot pass through the cell wall membrane.…”