Comparative physiological and transcriptomic analyses illuminate common mechanisms by which silicon alleviates cadmium and arsenic toxicity in rice seedlings

Chen, Huiqiong; Liang, Xiaoyu; Gong, Xiaomei; Reinfelder, John R.; Chen, Huamei; Sun, Chongjun; Liu, Xiulian; Zhang, Shuchang; Li, Fangbai; Liu, Chuanping; Zhao, Junliang; Yi, Jicai

doi:10.1016/j.jes.2021.02.030

Cited by 44 publications

(11 citation statements)

References 80 publications

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“…Notably, more than half of DEGs involved in POD and two highly expressed POD-encoding genes ( POD12.1 and POD 12.2 ) were induced by short-term Cd stress with higher expression in the Cd-tolerant variety. Besides its antioxidant properties, POD can also catalyze the synthesis of lignin, induce cell wall stiffening, and affect the transportation of heavy metals [ 28 , 30 , 31 ]. According to the physiological data, POD activity was significantly increased under Cd stress and showed higher activity in the Cd-tolerant variety, suggesting POD genes such as POD12.1 and POD 12.2 may be responsible for Cd tolerance and low translocation in Cd-tolerant variety.…”

Section: Discussionmentioning

confidence: 99%

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Li¹,

Zhang²,

Zhang³

et al. 2022

IJMS

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Broomcorn millet (Panicum miliaceum L.) has great potential in Cd phytoextraction, but its mechanisms are largely unknown. Two contrasting broomcorn millet varieties, ‘Ningmi6’ (Cd-sensitive variety) and ‘4452’ (Cd-tolerant variety), were investigated through morphological, physiological, and transcriptomic analyses to determine the factors responsible for their differential Cd tolerance and translocation. The Cd-tolerant variety can accumulate more Cd, and its cell wall and vacuole component Cd proportions were higher compared with the Cd-sensitive variety. Under Cd stress, the glutathione content and peroxidase activity of the Cd-tolerant variety were significantly higher than those of the Cd-sensitive variety. Additionally, weighted gene co-expression network analysis (WGCNA) revealed hub modules that were associated with Cd stress and/or variety. Notably, genes involved in these hub modules were significantly enriched for roles in glutathione metabolism, phenylpropanoid biosynthesis, ABC transport, and metal ion transport process. These results suggested that regulation of genes associated with cell wall precipitation and vacuole compartmentalization may increase Cd tolerance and reduce Cd translocation in the Cd-tolerant variety, although it can absorb more Cd. This study provides a foundation for exploring molecular mechanisms of Cd tolerance and transport in broomcorn millet and new insights into improving Cd phytoremediation with this crop through genetic engineering.

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Section: Discussionmentioning

confidence: 99%

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Li¹,

Zhang²,

Zhang³

et al. 2022

IJMS

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“…To explore this knowledge gap, the Cd-related functions of a recently identified Cd-responsive rice thionin gene, provisionally named OsThi9, 31 were investigated in the present study. Phylogenetic analysis recovered OsThi9 in a clade distinct from the γ-thionins (including the previously reported plant defensins AtPDF2.5, AtPDF2.6, AhPDF1.1, CAL1, CAL2, and DEF8; Figure 1a), suggesting that OsThi9 is a defensindissimilar or non-defensin thionin.…”

Section: Role Of Osthi9 and Associated Mechanisms In The Regulation O...mentioning

confidence: 99%

“…Previously, we identified a Cd-induced thionin gene in rice, temporarily named OsThi9 (Os06g0514800), 31 that encodes a defensin-dissimilar thionin protein (Thionin 9) with a low similarity to the amino acid sequences of CAL1, CAL2, and DEF8. Here, to clarify the role played by OsThi9 in the mitigation of Cd toxicity and accumulation in rice, as well as the associated underlying mechanisms, we characterized OsThi9 expression patterns and the effects of OsThi9 overexpression and knockout.…”

Section: ■ Introductionmentioning

confidence: 99%

“…It is thus important to investigate whether the defensin-dissimilar thionins (i.e., α/β-thionins with low similarity in protein sequence and structure to the defensins) play a role in the regulation of Cd tolerance or accumulation in rice. Previously, we identified a Cd-induced thionin gene in rice, temporarily named OsThi9 (Os06g0514800), 31 that encodes a defensin-dissimilar thionin protein (Thionin 9) with a low similarity to the amino acid sequences of CAL1, CAL2, and DEF8. Here, to clarify the role played by OsThi9 in the mitigation of Cd toxicity and accumulation in rice, as well as the associated underlying mechanisms, we characterized OsThi9 expression patterns and the effects of OsThi9 overexpression and knockout.…”

Section: ■ Introductionmentioning

confidence: 99%

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Plant Defensin-Dissimilar Thionin OsThi9 Alleviates Cadmium Toxicity in Rice Plants and Reduces Cadmium Accumulation in Rice Grains

Liu

Gong

Zhou

et al. 2023

J. Agric. Food Chem.

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Thionins are important antibacterial peptides in plants. However, the roles of plant thionins, especially the defensindissimilar thionins, in alleviating heavy-metal toxicity and accumulation remain unclear. Here, cadmium (Cd)-related functions and mechanisms of the defensin-dissimilar rice thionin OsThi9 were investigated. OsThi9 was significantly upregulated in response to Cd exposure. OsThi9 was localized to the cell wall and was shown to bind Cd; these characters help to increase Cd tolerance. In Cdexposed rice plants, OsThi9 overexpression significantly increased cell wall Cd binding, decreasing upward Cd translocation and subsequent Cd accumulation in shoots and straw, while OsThi9 knockout had inverse effects. Importantly, in rice plants grown in Cd-contaminated soils, OsThi9 overexpression significantly reduced Cd accumulation in brown rice (decrease of ≥ 51.8%) without negatively impairing the crop yield and essential elements. Thus, OsThi9 plays an important role in the alleviation of Cd toxicity and accumulation and has significant potential for developing low-Cd rice.

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“…Wang et al(2017) reported that OsARM1, an MYB transcription factor was found to regulate the plant response to arsenic stress. Also,Chen et al (2021) proposed that an MYB factor gene OsMYBS1 gene is responsible for Si-mediated As and Cd stress response by regulating the expression of the transporter genes through GA or ABA signals(Lu et al 2002;Chen et al 2017. Additionally, a transcriptome study in wheat found that MYB factor genes formed a signi cant subset of the genes which were differentially expressed when the seedlings were treated with Si.…”

mentioning

confidence: 99%

Genome wide association studies and candidate gene mining for understanding genetic basis of straw silica content in a set of Oryza nivara (Sharma et Shastry) accessions

Gowda

Sharma

Gill

et al. 2023

Preprint

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Background Rice is a high silicon-accumulator. Silicon (Si) is designated as a beneficial element associated with multiple positive effects on crop plants. However, presence of high silica content is detrimental in rice straw and its management, hampering its use as animal feed and as raw material in multiple industries. Rice straw management is a serious concern in North-Western India and it is eventually burned in situ by the farmers contributing to air pollution. A practical solution to the said issue could lie in reducing the silica content in rice, while also attaining sound plant growth. Results A set of 258 Oryza nivara accessions along with 25 cultivated varieties were used to assess the variation for straw silica content using molybdenum blue colorimetry method. Large and continuous variation was observed for straw silica content in O. nivara accessions, ranging from 5.08-16%, while it varied from 7.95–15.81% in the cultivated varieties. The O. nivara accessions containing 43–54% lower straw silica content than the currently prominent cultivated varieties in the region were identified. A set of 22,528 high-quality single nucleotide polymorphism (SNP) among the 258 O. nivara accessions were used for estimating population structure and genome wide association studies (GWAS). A weak population structure with 59% admixtures was identified among O. nivara accessions. Further, multi-locus GWAS revealed the presence of 14 marker trait associations (MTAs) for straw silica content, with six of them co-localizing with previously reported QTLs. Twelve out of the 14 MTAs showed statistically significant allelic differences. Thorough candidate gene analyses among the QTL regions revealed the presence of promising candidate genes, including ABC transporter, Casparian thickening genes, multi-drug and toxin extrusion (MATE) protein, F-box domains, and MYB-transcription factor domains. Besides ortho-QTLs among rice and maize genomes were also identified which could open up ways for further genetic analysis of this trait. Conclusion The findings of the study could aid in further understanding and characterizing genes for Si transport and regulation in the plant body. The donors carrying the alleles for lower straw silica content can be used in further marker-assisted breeding programmes in order to develop rice varieties with lower silica content along with higher yield potential.

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Comparative physiological and transcriptomic analyses illuminate common mechanisms by which silicon alleviates cadmium and arsenic toxicity in rice seedlings

Cited by 44 publications

References 80 publications

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Plant Defensin-Dissimilar Thionin OsThi9 Alleviates Cadmium Toxicity in Rice Plants and Reduces Cadmium Accumulation in Rice Grains

Genome wide association studies and candidate gene mining for understanding genetic basis of straw silica content in a set of Oryza nivara (Sharma et Shastry) accessions

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