Comparative physiology and biochemistry of rat and rabbit urinary bladder

Damaser, Margot S.; Whitbeck, Catherine; Barreto, Mark; Horan, Patrick; Benno, H.; O’Connor, Laura; Levin, Robert M.

doi:10.1046/j.1464-410x.2000.00444.x

Cited by 11 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sprague-Dawley rats were chosen as an animal model because the Ca-ATPase activity of these rats is five times that of rabbits, they have high energy consumption, and they have a hyperactive bladder that is very easily examined for neurological disorders caused by stress [9]. In addition, the main mechanism for human bladder contraction is cholinergic with an insignificant proportion of purinergic contraction [10].…”

Section: Discussionmentioning

confidence: 99%

Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue

et al. 2010

View full text Add to dashboard Cite

Purpose:We examined the effect of stress on the pathophysiology of bladder stability in terms of enzyme levels, Rho-kinase, and bladder relaxation. Materials and Methods: A total of 48 female Sprague-Dawley rats were studied in scheduled stress environments for 7, 14, and 28 days; 24 rats were in the control group and 24 rats were in the test (stressed) group. Results: Estrogen decreased significantly whereas testosterone and dopamine increased significantly in the stress group (p＜0.05). Rho-kinase was significantly increased in the rats exposed to stress stimuli for 14 days (p＜0.05). Collagen types I and III in the bladder tissue were significantly higher in rats exposed to stress for 14 days and 28 days (collagen type I in the 14-day group, p＜0.01; collagen type I in the 28-day group, p＜0.05; collagen type III in the 14-day and 28-day groups, p＜0.05). Voiding frequency increased significantly as the duration of stress exposure was prolonged, in addition to a significant decrease in volume per voiding (p＜0.05). Conclusions:The changes observed in micturition pattern, factors that contribute to smooth muscle contraction, and relaxation in the female rat bladder support the hypothesis that stress affects bladder stability.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Among them, rats and rabbits are the most commonly used species. There are numerous studies that provide detailed information regarding urinary bladder morphology, cellular biochemistry, contractile function and normal micturition in these species (Levin et al ., 1983, 1990, 1994; Longhurst et al ., 1990; Steers, 1994; Damaser et al ., 2000). In mammals, acetylcholine is the principal neurotransmitter that acts at muscarinic receptors of the detrusor muscle leading to contraction of the smooth muscle; therefore, compounds that act as antagonists at these receptors would reduce bladder overactivity.…”

Section: Discussionmentioning

confidence: 99%

AE9C90CB: a novel, bladder‐selective muscarinic receptor antagonist for the treatment of overactive bladder

Sinha¹,

Gupta²,

Malhotra³

et al. 2010

British J Pharmacology

View full text Add to dashboard Cite

Background and purpose: AE9C90CB (N-[(1R, 5S, 6R)-3-azabicyclo [3.1.0] hex-6-ylmethyl]-2-hydroxy-N-methyl-2, 2-diphenylacetamide), a novel muscarinic receptor antagonist, was synthesized for the treatment of overactive bladder. Here we describe the in vitro and in vivo profiles of AE9C90CB for action in bladder over salivary gland and compare it with four agents already in clinical use (tolterodine, oxybutynin, solifenacin and darifenacin). Experimental approach: Radioligand binding assay and isolated tissue-based functional assay were used to evaluate affinity, potency, and receptor subtype selectivity of compounds. Inhibition of carbachol-induced increase in intravesicular pressure and salivary secretion were measured in anaesthetized rabbits to assess the functional selectivity. Key results: In vitro radioligand binding study using human recombinant muscarinic receptors showed that AE9C90CB had greater affinity for M3 muscarinic receptors with pKi of 9.90 Ϯ 0.11 and was 20-fold more selective for M3 than for M2 muscarinic receptors. AE9C90CB exhibited an unsurmountable antagonism on rat bladder strips (pKB, 9.13 Ϯ 0.12). In anaesthetized rabbits after intravenous administration, AE9C90CB dose dependently inhibited carbachol-induced increase in intravesicular pressure and salivary secretion, and exhibited functional selectivity for urinary bladder over salivary gland which was ninefold better than that of oxybutynin. Conclusions and implications:We have identified AE9C90CB, a compound exhibiting moderate selectivity for M3 over M2 receptors but greater selectivity for urinary bladder over salivary gland than oxybutynin, tolterodine, solifenacin and darifenacin. Therefore, AE9C90CB may be a promising compound for the treatment of overactive bladder with reduced potential to cause dry mouth than currently available antimuscarinic drugs. British Journal of Pharmacology IntroductionOveractive bladder (OAB) is one of the most common causes of bladder control problems. It derives from the uncontrolled activity of the detrusor muscle during bladder filling, leading to the symptoms of urinary urgency and increased frequency of micturition with or without incontinence . Symptoms of OAB are highly prevalent in men and women of all ages, and the risk of developing this condition increases with age (Wein and Rovner, 1999), with a sharp increase among patients 40 years of age or older (Stewart et al., 2003). OAB is a disruptive condition that not only can cause embarrassment but also can have a significant impact on the patient's quality of life (Abrams et al., 2000;Bogner et al., 2002). Despite the disturbing nature of OAB, only 20% of patients are on medical treatments, and in addition, OAB is an under-diagnosed condition. Although not life-threatening, the quality of life of patients with OAB is poorer than that of diabetics (Liberman et al., 2001).Urinary bladder contraction is predominantly under the control of the parasympathetic system (de Groat et al., 1981), where the primary input is via the muscarinic cholinoce...

show abstract

“…The urinary bladder is a smooth muscle (SM) organ whose function is to collect and store urine at low intravesical pressures and then to periodically expel the urine via highly coordinated, sustained contractions. [1,2] Bladder function depends upon several factors including state of innervation, structure of the organ as a whole, contractile response of the SM elements to autonomic stimulation and availability of metabolic energy sources (cytosolic ATP and mitochondrial oxidative metabolites). [1,2] These factors are intimately associated with an alteration in bladder function in one and can induce substantial adaptive changes in the others.…”

Section: Introductionmentioning

confidence: 99%

The effect of α-and δ-tocopherol-lipoic acid ester co-drugs on the response of the rabbit bladder to in vitro ischemia/reperfusion

Levin

Schuler

Leggett

et al. 2019

Turkish Journal of Urology

View full text Add to dashboard Cite

show abstract

Comparative physiology and biochemistry of rat and rabbit urinary bladder

Cited by 11 publications

References 30 publications

Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue

Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue

AE9C90CB: a novel, bladder‐selective muscarinic receptor antagonist for the treatment of overactive bladder

The effect of α-and δ-tocopherol-lipoic acid ester co-drugs on the response of the rabbit bladder to in vitro ischemia/reperfusion

Contact Info

Product

Resources

About