Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance

Faghani, Elham; Gharechahi, Javad; Komatsu, Setsuko; Mirzaei, Mehdi; Khavari-Nejad, Ramzan Ali; Najafi, Farzaneh; Farsad, Laleh Karimi; Salekdeh, Ghasem Hosseini

doi:10.1016/j.jprot.2014.10.018

Cited by 106 publications

(73 citation statements)

References 66 publications

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“…Faghani et al . [118] reported an increase in the levels of ferredoxin-NADP reductase in the leaves of the drought-stressed wheat cultivar with higher resistance to this stressor but not in leaves in the drought-sensitive cultivar. Li et al .…”

Section: Discussionmentioning

confidence: 99%

“…Faghani et al . [118] reported decreased levels of the γ subunit of ATP synthase in a drought-sensitive cultivar of wheat whereas the amounts of this protein in leaves of drought-tolerant plants did not change. However, other authors [99, 114, 121] observed a down-regulation of the α subunit in leaves of both drought-tolerant and -sensitive genotypes of French bean, eucalyptus or sugarcane plants.…”

Section: Discussionmentioning

confidence: 99%

“…found that plants of Stipa purpurea that were adapted to a more arid environment were able to increase levels of various subunits of both cytosolic and chloroplast ribosomes in response to drought but this phenomenon was not observed in a more sensitive variant of the same species [109]. Another study dealing with intraspecific differences in wheat subjected to drought conditions documented a decrease in one protein of the 60S ribosomal subunit in a sensitive cultivar but not in a tolerant one [118]. Regarding chloroplast ribosomes, some authors have observed an increase in some proteins of both large and small ribosomal subunits to be associated with a better drought tolerance [96, 99, 101, 107], although this was not true for all proteins belonging to this category [96, 107].…”

Section: Discussionmentioning

confidence: 99%

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The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize

et al. 2017

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A comparative analysis of various parameters that characterize plant morphology, growth, water status, photosynthesis, cell damage, and antioxidative and osmoprotective systems together with an iTRAQ analysis of the leaf proteome was performed in two inbred lines of maize (Zea mays L.) differing in drought susceptibility and their reciprocal F1 hybrids. The aim of this study was to dissect the parent-hybrid relationships to better understand the mechanisms of the heterotic effect and its potential association with the stress response. The results clearly showed that the four examined genotypes have completely different strategies for coping with limited water availability and that the inherent properties of the F1 hybrids, i.e. positive heterosis in morphological parameters (or, more generally, a larger plant body) becomes a distinct disadvantage when the water supply is limited. However, although a greater loss of photosynthetic efficiency was an inherent disadvantage, the precise causes and consequences of the original predisposition towards faster growth and biomass accumulation differed even between reciprocal hybrids. Both maternal and paternal parents could be imitated by their progeny in some aspects of the drought response (e.g., the absence of general protein down-regulation, changes in the levels of some carbon fixation or other photosynthetic proteins). Nevertheless, other features (e.g., dehydrin or light-harvesting protein contents, reduced chloroplast proteosynthesis) were quite unique to a particular hybrid. Our study also confirmed that the strategy for leaving stomata open even when the water supply is limited (coupled to a smaller body size and some other physiological properties), observed in one of our inbred lines, is associated with drought-resistance not only during mild drought (as we showed previously) but also during more severe drought conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize

et al. 2017

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“…Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought stress. Recently, a few published studies have been applied to describe proteome changes in different wheat genotypes under drought stress [41–45]. A small set of drought-inducible proteins was also identified from these studies in various wheat organs including seedling leaves, stems, roots and grains.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Cheng

Wang

et al. 2016

BMC Plant Biol

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BackgroundDrought stress is one of the most adverse environmental constraints to plant growth and productivity. Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought. This study attempted to extend findings regarding the potential proteomic dynamics in wheat under drought stress and to enrich the research content of drought tolerance mechanism.ResultsA comparative proteomics approach was applied to analyze proteome change of Xihan No. 2 (a drought-tolerant cultivar) and Longchun 23 (a drought-sensitive cultivar) subjected to a range of dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) using 2-DE, respectively. Quantitative image analysis showed a total of 172 protein spots in Xihan No. 2 and 215 spots from Longchun 23 with their abundance significantly altered (p < 0.05) more than 2.5-fold. Out of these spots, a total of 84 and 64 differentially abundant proteins were identified by MALDI-TOF/TOF MS in Xihan No. 2 and Longchun 23, respectively. Most of these identified proteins were involved in metabolism, photosynthesis, defence and protein translation/processing/degradation in both two cultivars. In addition, the proteins involved in redox homeostasis, energy, transcription, cellular structure, signalling and transport were also identified. Furthermore, the comparative analysis of drought-responsive proteome allowed for the general elucidation of the major mechanisms associated with differential responses to drought of both two cultivars. These cellular processes work more cooperatively to re-establish homeostasis in Xihan No. 2 than Longchun 23. The resistance mechanisms of Xihan No. 2 mainly included changes in the metabolism of carbohydrates and amino acids as well as in the activation of more antioxidation and defense systems and in the levels of proteins involved in ATP synthesis and protein degradation/refolding.ConclusionsThis study revealed that the levels of a number of proteins involved in various cellular processes were affected by drought stress in two wheat cultivars with different drought tolerance. The results showed that there exist specific responses to drought in Xihan No. 2 and Longchun 23. The proposed hypothetical model would explain the interaction of these identified proteins that are associated with drought-responses in two cultivars, and help in developing strategies to improve drought tolerance in wheat.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0871-8) contains supplementary material, which is available to authorized users.

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“…During the grain filling period, DS induces early senescence and shortens the length of this period. High DS also triggers photosynthesis cessation, metabolism disruption, and ultimately cell death …”

Section: Introductionmentioning

confidence: 99%

Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat

Wang

et al. 2015

J. Sci. Food Agric.

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Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance

Cited by 106 publications

References 66 publications

The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize

The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize

Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat

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