Comparative physiology of Malpighian tubules: form and function

Cintra-Socolowski, Priscila; Nocelli, Roberta Cornélio Ferreira; Roat, Thaisa Cristina; Silva-Zacarín, Elaine C.M.; Malaspina, Osmar

doi:10.2147/oaip.s72060

Cited by 20 publications

(11 citation statements)

References 69 publications

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“…Malpighian tubules possess a mechanism of active transport of toxins and, along with the midgut, respond rapidly to their presence [46,47]. This property makes them great biomarkers for ecotoxicological studies [46] as was shown in other studies on insects [33,34]. Vacuolization of the epithelial cells in Malpighian tubules was noted in bees exposed to insecticides, as an indicator of the initiation of the cell death process [48].…”

Section: Discussionmentioning

confidence: 71%

“…However, vacuolization of epithelial cells was recorded in larvae exposed to CeO2 and Fe3O4 nanoparticles. Malpighian tubules possess a mechanism of active transport of toxins and, along with the midgut, respond rapidly to their presence [46,47]. This property makes them great biomarkers for ecotoxicological studies [46] as was shown in other studies on insects [33,34].…”

Section: Discussionmentioning

confidence: 83%

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Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Stojanović

Milošević

Vitorovic

et al. 2021

ARCH BIOL SCI BELGRA

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As the production of metal-based nanomaterials increases, it is inevitable that nano-scale products and byproducts will enter the aquatic environment. In terms of global production, the most abundant nano-oxides are TiO2, CeO2 and Fe3O4 nanoparticles. Chironomus riparius is commonly used for ecotoxicological assessment and defining its histopathological biomarkers that showcase the toxic effect of tested nanoparticles should lead to a better understanding of the consequences of nanomaterial accumulation in aquatic ecosystems. In this study, a histological description of the digestive and excretory systems as well as the fat body structure of C. riparius larvae is provided. In addition, potential histological biomarkers of nano-oxide toxicity were determined based on the obtained histopathological alterations in organs. Vacuolization was observed in epithelial cells of midgut region I that were treated with nano-Fe3O4 as well as in Malpighian tubules treated with nano-Fe3O4 and nano-CeO2. Larvae exposed to nano-TiO2 showed alterations in the fat body and midgut region II tissue architecture. Additionally, shortening of the intestinal brush border was determined in groups exposed to nano-Fe3O4. These results reveal the high sensitivity of these organs, which can be used as biomarkers in histopathological assessment and therefore lead to further improvement of existing methodology in ecotoxicological studies.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 83%

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Stojanović

Milošević

Vitorovic

et al. 2021

ARCH BIOL SCI BELGRA

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“…Until relatively recently, our understanding of diuresis in insects relied heavily on in vitro work, which has indeed enabled models to be developed on the neurohormonal control of diuresis. Access to the insect genomes that have been sequenced over the last 20 years or so, and advances in molecular techniques, have, however, revolutionized insect (neuro)endocrinology, and functional genomics and genetics, including gene micro-arrays, transcriptomes, mutations, double stranded RNA (dsRNA) and sophisticated peptidomics ('omics in general) are enabling complex regulatory processes to be exquisitely dissected and defined (2,4,(9)(10)(11)(12)(13). So too, the basic model of post-prandial diuresis in R. prolixus, with its diuretic and antidiuretic hormones, coupled to the second messengers cAMP, cGMP and IP 3 , and in turn acting via predicted ion channels, transporters and aquaporins is here validated and confirmed by transcriptomics; but transcriptomics has also revealed how much more still needs to be understood.…”

Section: Final Thoughtsmentioning

confidence: 99%

“…The post-genomic era has brought forth great advances in our understanding of the diuretic processes in insects, enabling the identification and quantification of thousands of target genes in a single experiment, as well as enabling comparative genomic studies. Microarray and transcriptome analyses of Malpighian tubules have also reinforced the major roles that Malpighian tubules play in detoxification and immunity, and in tolerance to stress and desiccation (2,4,(9)(10)(11)(12)(13). The R. prolixus genome has been sequenced and reported (14), bringing the post-genomic era to bear on this historic model insect.…”

Section: Introductionmentioning

confidence: 96%

Fluid Secretion by Malpighian Tubules of Rhodnius prolixus: Neuroendocrine Control With New Insights From a Transcriptome Analysis

et al. 2021

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Rhodnius prolixus (the kissing bug and a major vector of Chagas disease) is an obligate blood feeder that in the case of the fifth instar consumes up to 10 times its unfed body weight in a single 20-minute feed. A post-prandial diuresis is initiated, within minutes of the start of gorging, in order to lower the mass and concentrate the nutrients of the meal. Thus, R. prolixus rapidly excretes a fluid that is high in NaCl content and hypo-osmotic to the hemolymph, thereby eliminating 50% of the volume of the blood meal within 3 hours of gorging. In R. prolixus, as with other insects, the Malpighian tubules play a critical role in diuresis. Malpighian tubules are not innervated, and their fine control comes under the influence of the neuroendocrine system that releases amines and neuropeptides as diuretic or antidiuretic hormones. These hormones act upon the Malpighian tubules via a variety of G protein-coupled receptors linked to second messenger systems that influence ion transporters and aquaporins; thereby regulating fluid secretion. Much has been discovered about the control of diuresis in R. prolixus, and other model insects, using classical endocrinological studies. The post-genomic era, however, has brought new insights, identifying novel diuretic and antidiuretic hormone-signaling pathways whilst also validating many of the classical discoveries. This paper will focus on recent discoveries into the neuroendocrine control of the rapid post-prandial diuresis in R. prolixus, in order to emphasize new insights from a transcriptome analysis of Malpighian tubules taken from unfed and fed bugs.

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“…The peritrophic membrane is continuously replaced as it is shed and serves to facilitate digestion, and as a protective barrier from damage by food particles and microbial invasion (Engel & Moran, 2013b). At the pylorus, the Malpighian tubules excrete nitrogenous waste and electrolytes from the hemolymph presenting a nutrient-rich environment for microbes (Cintra-Socolowski, Nocelli, Roat, Silva-Zacarin, & Malaspina, 2016;Cohen, Sawyer, Peterson, Dow, & Fox, 2020). Midgut function including the production of peritrophic membrane decreases significantly in foragers (Harwood & Amdam, 2021), but the impact on disease susceptibility and the gut microbiome are unknown.…”

Section: Introductionmentioning

confidence: 99%

Social structure affects the gut microbiota in honey bees: The physiological cost of precocious foraging

Copeland

Mott

Anderson

2022

Preprint

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Honey bees exhibit an elaborate social structure that corresponds with worker aging and division of labor. Young workers perform tasks inside the hive, while older workers forage outside the hive. Critical to colony fitness, the work force can respond rapidly to changes in the environment or colony demography and assume emergency tasks, resulting in young foragers or old nurses. We hypothesized that social structure affects the microbiome, more specifically, that behavioral task independent of age would generate differences in gut microbiota and host physiology. We used high throughput sequencing to track gut microbial succession, and measured gene expression and oxidative protein damage associated with behavioral task and age. We found that both age and behavioral task could explain differences in midgut and ileum microbiota, but host gene expression was best explained by an interaction of task and age. An extended nursing role in early life stabilized the ratio abundance of G. apicola and S. alvi in the ileum, while precocious foraging resulted in poor G. apicola establishment, nutrient deficient gene expression, and increased oxidative damage. Our results suggest that the physiological cost of early foraging is extreme, and highlight the progression of colony dwindling, a common but misunderstood process.

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Comparative physiology of Malpighian tubules: form and function

Cited by 20 publications

References 69 publications

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Fluid Secretion by Malpighian Tubules of Rhodnius prolixus: Neuroendocrine Control With New Insights From a Transcriptome Analysis

Social structure affects the gut microbiota in honey bees: The physiological cost of precocious foraging

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