Comparative Phytochemical Profiling (GC-MS and HPLC) and Evaluation of Antioxidant Activities of Wild, in Vitro Cultured and Greenhouse Plants of Kaempferia grandifolia Saensouk and Jenjitt and Kaempferia siamensis Sirirugsa; Rare Plant Species in Thailand

Nonthalee, Sukanya; Maneechai, Suthira; Saensouk, Surapon; Saensouk, Piyaporn

doi:10.1177/09731296221145066

Cited by 2 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results were contrary to Yeap et al (2017) who reported boesenboxide, crotepoxide, 2ˊ-hydroxy-4,4ˊ,6ˊ-trimethoxychalcone, kaempfolienol and zeylenol in the rhizome of K. angustifolia from Java, Indonesia using liquid chromatography-mass spectrometry (LC-MS), while Tang et al (2014) also reported 8 phytochemical compounds;kaempfolienol, crotepoxide, boesenboxide, 2'-hydroxy-4,4',6'-trimethoxychalcone, zeylenol, 6-methylzeylenol, (24S)-24-methyl-5lanosta-9(11), 25-dien-3 -ol and sucrose, -sitosterol, and its glycoside in the rhizome of K. angustifolia from Java, Indonesia using mass spectrometry (MS). Nonthalee et al (2023) used comparative phytochemical profiling (GC-MS and HPLC) to evaluate the antioxidant activities of wild, in vitro cultured, and greenhouse plants of Kaempferia grandifolia and Kaempferia siamensis from Thailand. Their GC-MS results revealed 38 compounds in K. grandifolia and 19 compounds in K. siamensis.…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe

SAENSOUK,

YAOWACHAI,

CHUMROENPHAT

et al. 2023

Not Bot Horti Agrobo

View full text Add to dashboard Cite

Kaempferia angustifolia Roscoe is a medicinal plant in the family Zingiberaceae, with wild occurrence decreasing as a result of both natural and human threats. Tissue culture techniques provide an alternative method of propagation for mass production. Microshoots of K. angustifolia (1 cm in length) were cultured on MS medium supplemented with various concentrations of cytokinins (BA and Kinetin) and auxins (NAA and IAA) for 8 weeks. Phytochemical profiles were evaluated by total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ⁄ antioxidant power (FRAP) assay) and Gas chromatography–mass spectrometry (GC–MS) analysis. Maximum number of shoots was observed in medium supplemented with 2 mg/l BA plus 0.5 mg/l NAA (6.33 shoots/explant) and optimal rooting was induced in MS medium with 2 mg/l Kinetin plus 1 mg/l NAA (35.70 roots/explant). Highest numbers of shoots and roots were obtained when cultured on liquid MS medium supplemented with 2 mg/l BA, 2 mg/l Kinetin and 1 mg/l NAA (8.73 shoots/explant and 29.67 roots/explant, respectively). Regenerated plantlets of K. angustifolia were transferred to pots containing different types of plant materials under natural conditions for 8 weeks. Optimal survival rate was 100% when transplanting K. angustifolia to soil, sand, soil and sand (1:1) or soil and small pieces of rock (1:1) ratio. The methanol extract of leaves K. angustifolia from natural plants and in vitro derives plants showed a significantly higher amount of TFC and antioxidant activity. GC-MS analysis identified 52 phytochemical compounds in leaves of K. angustifolia. This study may be helpful to increase the value of commercial production of K. angustifolia, pharmaceutical and medicinal purpose.

show abstract

Section: Discussion Discussion Discussionmentioning

confidence: 99%

In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe

SAENSOUK,

YAOWACHAI,

CHUMROENPHAT

et al. 2023

Not Bot Horti Agrobo

View full text Add to dashboard Cite

show abstract

A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of Kaempferia grandifolia Saensouk & Jenjittikul—A Rare Plant Species in Thailand

Nonthalee,

Saensouk,

Maneechai

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Kaempferia grandifolia Saensouk & Jenjitikul (Zingiberaceae), a rare plant species in Thailand, is commonly used as a medicinal plant, while the young leaves are cooked and eaten as a vegetable. This study focused on the in vitro propagation of K. grandifolia plants to induce the development of shoots, roots, microrhizomes, and acclimatization. The genetic variations between plants grown under in vitro propagation and plants grown in nature were also assessed. An efficient protocol was developed for in vitro propagation of K. grandifolia using microshoots (0.5 cm in length). The optimal responses for shoot multiplication (4.78 ± 1.15 shoots/explant) and root induction (11.56 ± 1.39 roots/explant) were obtained within 8 weeks in Murashige and Skoog (MS) medium supplemented with 3 mg/L of 6-benzylamino purine (BAP) plus 0.5 mg/L of NAA. Microrhizome induction was 80% when the shoot buds were cultured on MS medium supplemented with 2 mg/L of BAP, 1 mg/L of NAA, 11 µM of silver nitrate (AgNO3), and 70 g/L of sucrose after 20 weeks. The survival rate of plantlets was 100% when transplanted to soil and sand. Micropropagated and field-grown donor plants of K. grandifolia showed 91.49% genetic similarity through RAPD analysis.

show abstract

Comparative Phytochemical Profiling (GC-MS and HPLC) and Evaluation of Antioxidant Activities of Wild, in Vitro Cultured and Greenhouse Plants of Kaempferia grandifolia Saensouk and Jenjitt and Kaempferia siamensis Sirirugsa; Rare Plant Species in Thailand

Cited by 2 publications

References 22 publications

In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe

In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe

A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of Kaempferia grandifolia Saensouk & Jenjittikul—A Rare Plant Species in Thailand

Contact Info

Product

Resources

About