The present study was conducted to evaluate the phytotoxic effect of eugenol, a monoterpenoid alcohol, on some crop species and to determine its anatomical implications. For this study, three crops viz., Zea mays, Phaseolus aureus and Oryza sativa were selected. A laboratory bioassay was carried out to assess the phytotoxicity of eugenol on the crop species in a dose-dependent manner. Eugenol inhibited the early growth of the crop species determined by its effect on the radicle and plumule length. The radicle and plumule length were found to decrease with increase in concentration of eugenol in all the three crop species. Significant inhibition was observed at higher dose of eugenol treatment. The radicle inhibition was more pronounced as compared to the plumule. Zea mays was found to be the most sensitive among the test plants. Further, significant alterations in the internal structure of roots of Zea mays as seen in the epidermal, cortical and vascular regions were observed due to eugenol treatment. Therefore, the phytotoxicity of eugenol towards crops is a significant step to determine its potential against weed species for its possible use as a bio-herbicide in the agro-ecosystems.